Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FRE64_RS13325 Genome accession   NZ_CP042326
Coordinates   2699856..2700968 (+) Length   370 a.a.
NCBI ID   WP_146296677.1    Uniprot ID   A0A5B8NPD2
Organism   Euhalothece natronophila Z-M001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2694856..2705968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRE64_RS13315 (FRE64_13315) bchL 2696366..2697247 (-) 882 WP_146296675.1 ferredoxin:protochlorophyllide reductase (ATP-dependent) iron-sulfur ATP-binding protein -
  FRE64_RS13320 (FRE64_13320) pilB/pilB1 2697716..2699740 (+) 2025 WP_146296676.1 GspE/PulE family protein Machinery gene
  FRE64_RS13325 (FRE64_13325) pilT 2699856..2700968 (+) 1113 WP_146296677.1 type IV pilus twitching motility protein PilT Machinery gene
  FRE64_RS13330 (FRE64_13330) - 2700983..2702203 (+) 1221 WP_146296678.1 type II secretion system F family protein -
  FRE64_RS13335 (FRE64_13335) - 2702211..2702786 (+) 576 WP_146296679.1 hypothetical protein -
  FRE64_RS13340 (FRE64_13340) - 2702977..2703222 (+) 246 WP_186708830.1 hypothetical protein -
  FRE64_RS13345 (FRE64_13345) - 2703248..2704852 (-) 1605 WP_146296681.1 radical SAM protein -
  FRE64_RS13350 (FRE64_13350) cysH 2705167..2705925 (+) 759 WP_222597829.1 phosphoadenosine phosphosulfate reductase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40924.91 Da        Isoelectric Point: 7.3006

>NTDB_id=377102 FRE64_RS13325 WP_146296677.1 2699856..2700968(+) (pilT) [Euhalothece natronophila Z-M001]
MELMIEDLMEQLVEKGGSDMHIQAGAPVYFRYNGKLQPVGDEPLTPQETQRLIFSMLNNNQRKQLEQSWELDCSYGVKGL
ARFRVNVYKERGCYAACLRALSSKIPNYEKLGLPEVVKEMAGRPRGLILVTGPTGSGKTTTLAAILDMINRSRYEHVLTV
EDPIEYVFPNEKCLFHQRQKGEDTKSFADALRAALREDPDIILVGELRDLETISLAVTAAETGHLVFGTLHTSSAAATVD
RMVDVFPADQQEQIRAQLSGSLIAVFSQCLPKKQNPKPGEFGRALAQEIMVVTPAIANQIREGKTSQIYSSIQTGMKLGM
QTMEQSLANWVNQGVISMEEALSKSSKPDELYRLTAGGAGKTKAKTNARR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=377102 FRE64_RS13325 WP_146296677.1 2699856..2700968(+) (pilT) [Euhalothece natronophila Z-M001]
ATGGAATTAATGATCGAAGACTTGATGGAACAGCTGGTGGAAAAAGGCGGTTCCGATATGCACATTCAAGCTGGCGCACC
TGTTTATTTTCGCTATAACGGGAAACTGCAACCAGTTGGTGATGAACCCCTAACTCCTCAAGAAACCCAACGTTTAATTT
TTAGTATGCTTAATAATAATCAACGTAAGCAACTAGAACAAAGTTGGGAATTAGATTGTTCTTATGGGGTAAAAGGACTG
GCTCGTTTTCGGGTTAATGTTTATAAAGAACGGGGATGTTACGCCGCTTGTTTACGGGCTTTATCTTCAAAAATTCCGAA
TTATGAAAAGTTAGGGTTACCAGAAGTGGTTAAAGAAATGGCTGGTCGCCCTCGGGGGTTAATTTTAGTGACTGGCCCCA
CTGGTTCTGGGAAAACTACCACCCTAGCAGCGATTTTAGACATGATTAATCGTAGTCGCTATGAGCACGTTTTAACAGTA
GAAGATCCTATTGAGTATGTTTTTCCCAATGAAAAATGTCTATTTCACCAACGACAAAAAGGGGAAGATACTAAAAGTTT
TGCTGATGCTTTGAGAGCAGCTTTACGGGAAGACCCAGATATTATTCTTGTTGGTGAATTACGGGACTTAGAAACAATTT
CTCTAGCGGTTACGGCAGCAGAAACTGGTCACTTAGTTTTTGGTACATTACATACCAGTTCTGCAGCGGCAACGGTTGAC
CGTATGGTGGATGTGTTTCCTGCCGACCAACAAGAGCAAATTCGCGCACAATTATCAGGTTCTTTAATTGCTGTATTCAG
TCAATGTTTACCGAAAAAACAAAATCCCAAGCCTGGGGAATTTGGACGCGCTTTAGCCCAAGAAATTATGGTGGTTACTC
CTGCCATTGCTAACCAAATTCGAGAAGGCAAAACCTCACAAATTTATTCCTCCATTCAAACTGGGATGAAACTAGGAATG
CAAACCATGGAACAGTCATTAGCCAATTGGGTGAATCAAGGTGTGATTTCTATGGAGGAAGCCCTCTCTAAAAGCAGTAA
ACCAGATGAACTTTATCGTCTGACTGCAGGTGGCGCAGGTAAAACCAAAGCAAAAACAAATGCTCGTCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8NPD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

53.913

93.243

0.503
  pilT Legionella pneumophila strain Lp02

53.913

93.243

0.503
  pilT Pseudomonas aeruginosa PAK

51.585

93.784

0.484
  pilT Acinetobacter nosocomialis M2

51.009

93.784

0.478
  pilT Acinetobacter baumannii D1279779

51.009

93.784

0.478
  pilT Acinetobacter baumannii strain A118

51.009

93.784

0.478
  pilT Acinetobacter baylyi ADP1

50.432

93.784

0.473
  pilT Pseudomonas stutzeri DSM 10701

50.432

93.784

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.541

98.919

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.44

92.162

0.465
  pilT Vibrio cholerae strain A1552

50.44

92.162

0.465
  pilT Neisseria meningitidis 8013

46.418

94.324

0.438
  pilT Neisseria gonorrhoeae MS11

46.132

94.324

0.435
  pilU Pseudomonas stutzeri DSM 10701

38.31

95.946

0.368

Multiple sequence alignment