Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FRE64_RS13320 Genome accession   NZ_CP042326
Coordinates   2697716..2699740 (+) Length   674 a.a.
NCBI ID   WP_146296676.1    Uniprot ID   A0A5B8NPF0
Organism   Euhalothece natronophila Z-M001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2692716..2704740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRE64_RS13295 (FRE64_13295) - 2693497..2694030 (+) 534 WP_146296671.1 TIGR02281 family clan AA aspartic protease -
  FRE64_RS18125 - 2694136..2694267 (-) 132 WP_281286877.1 hypothetical protein -
  FRE64_RS13300 (FRE64_13300) petN 2694289..2694378 (-) 90 WP_146296672.1 cytochrome b6-f complex subunit PetN -
  FRE64_RS17555 - 2694682..2694825 (-) 144 WP_186708828.1 hypothetical protein -
  FRE64_RS13305 (FRE64_13305) - 2694841..2696262 (-) 1422 WP_146296673.1 ferredoxin:protochlorophyllide reductase (ATP-dependent) subunit N -
  FRE64_RS13315 (FRE64_13315) bchL 2696366..2697247 (-) 882 WP_146296675.1 ferredoxin:protochlorophyllide reductase (ATP-dependent) iron-sulfur ATP-binding protein -
  FRE64_RS13320 (FRE64_13320) pilB/pilB1 2697716..2699740 (+) 2025 WP_146296676.1 GspE/PulE family protein Machinery gene
  FRE64_RS13325 (FRE64_13325) pilT 2699856..2700968 (+) 1113 WP_146296677.1 type IV pilus twitching motility protein PilT Machinery gene
  FRE64_RS13330 (FRE64_13330) - 2700983..2702203 (+) 1221 WP_146296678.1 type II secretion system F family protein -
  FRE64_RS13335 (FRE64_13335) - 2702211..2702786 (+) 576 WP_146296679.1 hypothetical protein -
  FRE64_RS13340 (FRE64_13340) - 2702977..2703222 (+) 246 WP_186708830.1 hypothetical protein -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 75439.81 Da        Isoelectric Point: 4.8460

>NTDB_id=377101 FRE64_RS13320 WP_146296676.1 2697716..2699740(+) (pilB/pilB1) [Euhalothece natronophila Z-M001]
MAQNSSGNKRRTALTTLRDSSPFGNKLIQAGYANSQQIEQARQQLSQAEGKQSFAEVISSITGKELPPELYRQYKKHHLF
ELKIYYGVESLDPEVTNLEDYAIEELIDDVIPLDACRRYKLLPLAKRETRPPSLVVGMVNPDDLTAQDDLTRILRPKGLK
PQRVVMTAEDYEKVITEYLDKQSAKQEEEAEEEQLDVTQDIANLDMEGLEESNEEADTDLENSLGNPQSAPIINVVNRML
IKALREGVSDIHVEPQEKYVRVRMRQDGVLQKSFDNLPLKIAPAIAARFKIMADLDIAERRMPQDGKIRRKYRGRNVDFR
VSTLPSRYGEKIVLRILDNSATQLGLDKLITDQESLDTVREMASRPFGLILVTGPTGSGKSTTLYSVLAERNDPGVNIST
AEDPIEYALPGITQVQVLREKGMDFASILRSFLRQDPDIILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARL
DEMGVEPFMISGSLIGVLAQRLMRRVCDKCRIPYNPSQEELARFGLSAAKEEEITFYKANVLKPDEIEPARQKGKLCSKC
NGTGYKGRAGVYEIMQMSETLQGLINSGAPTERIKEAAVEEGMKTLLAYSLNLVREGYTTLDEVERVTFTDSGLEAEIKA
KRKSSLTCNTCQAELQPEWLECPYCLTPRFPEDN

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=377101 FRE64_RS13320 WP_146296676.1 2697716..2699740(+) (pilB/pilB1) [Euhalothece natronophila Z-M001]
ATGGCACAAAATTCATCAGGTAACAAACGTCGCACCGCCTTAACAACTTTACGGGATTCTTCTCCGTTTGGAAATAAATT
AATCCAAGCTGGTTATGCCAATAGCCAGCAAATTGAACAAGCAAGACAACAATTATCACAAGCAGAAGGTAAGCAATCTT
TTGCTGAAGTGATTTCTTCTATTACAGGCAAAGAATTACCCCCGGAACTTTACCGTCAATACAAGAAACACCATTTATTT
GAACTCAAAATTTATTACGGGGTGGAATCTCTTGATCCTGAGGTGACAAACCTAGAAGACTATGCGATTGAAGAGTTAAT
TGATGATGTGATTCCCCTTGATGCTTGTCGCCGTTATAAGTTATTGCCTTTAGCGAAACGAGAGACACGCCCCCCTTCCT
TAGTAGTAGGAATGGTTAACCCAGATGACCTAACAGCTCAGGATGATCTCACCCGCATTCTTCGTCCTAAGGGGTTAAAA
CCTCAGCGTGTGGTGATGACAGCTGAGGACTATGAAAAGGTCATTACCGAGTATTTAGATAAACAATCAGCTAAACAAGA
AGAAGAAGCTGAAGAGGAGCAACTTGATGTTACTCAAGATATTGCTAACCTTGACATGGAAGGGTTAGAAGAGAGTAATG
AAGAAGCAGATACCGATTTAGAAAATTCTTTAGGGAACCCTCAATCTGCCCCCATTATTAATGTTGTAAATCGAATGTTA
ATTAAGGCTTTGCGAGAAGGCGTTTCCGATATTCATGTGGAGCCACAAGAAAAGTATGTTCGTGTGCGGATGCGACAAGA
TGGGGTGTTACAAAAATCATTTGATAACTTACCACTCAAAATTGCTCCCGCGATCGCGGCACGTTTTAAGATTATGGCTG
ACTTAGACATTGCTGAGCGTCGAATGCCTCAAGATGGGAAAATTCGTCGTAAATATAGGGGGCGAAATGTTGACTTTCGA
GTCAGTACCTTACCCAGTCGCTATGGCGAGAAAATTGTGTTGCGGATTTTAGATAATAGTGCCACCCAATTAGGACTGGA
TAAGCTCATTACTGACCAAGAGAGTCTTGATACAGTGCGAGAAATGGCAAGTCGTCCTTTTGGCTTAATTTTAGTAACAG
GTCCCACTGGGTCAGGAAAATCCACTACTCTATATTCTGTGTTAGCCGAGCGCAATGATCCAGGCGTGAATATTAGCACT
GCTGAAGACCCCATTGAATATGCTCTTCCCGGAATTACTCAGGTACAAGTATTGCGAGAAAAAGGCATGGACTTTGCCTC
GATTTTACGGTCATTCTTGCGTCAAGACCCTGATATTATTCTGGTGGGAGAGACCCGAGATAAAGAAACCGCAAAAACAG
CCATTGAAGCCGCATTAACAGGACACTTAGTATTAACGACATTACACACGAATGATGCCGCGGGCGCGATCGCGCGTTTA
GATGAAATGGGAGTAGAACCTTTTATGATTTCAGGATCATTAATTGGGGTTCTAGCTCAACGTCTAATGCGTCGTGTTTG
TGATAAATGTCGCATTCCCTATAACCCAAGTCAAGAAGAACTCGCTCGTTTTGGTTTATCGGCGGCAAAAGAAGAAGAAA
TAACCTTCTATAAAGCCAATGTCCTCAAACCTGATGAAATTGAACCCGCCCGTCAAAAAGGAAAACTCTGCTCTAAATGT
AATGGCACTGGCTATAAAGGGCGAGCTGGTGTTTATGAAATTATGCAGATGAGCGAAACTTTGCAGGGGTTAATTAATAG
TGGCGCACCCACAGAACGGATTAAAGAAGCGGCAGTAGAAGAAGGAATGAAAACCCTTCTTGCCTATAGTTTAAACTTAG
TTCGGGAAGGATATACCACACTTGATGAAGTAGAACGAGTGACCTTTACTGACTCTGGCTTAGAAGCAGAAATTAAAGCT
AAACGTAAAAGTTCTTTAACTTGTAATACTTGCCAGGCAGAACTACAACCAGAATGGTTAGAGTGTCCTTATTGTTTAAC
GCCACGTTTTCCAGAGGATAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(238-505)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(78-183)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8NPF0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.667

97.478

0.65

Multiple sequence alignment