Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Dpoa569_RS03640 Genome accession   NZ_CP042220
Coordinates   821534..822556 (+) Length   340 a.a.
NCBI ID   WP_042872598.1    Uniprot ID   A0A5B8I2U2
Organism   Dickeya poaceiphila strain NCPPB 569     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 816534..827556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Dpoa569_RS03610 (Dpoa569_0000730) hemW 817262..818401 (-) 1140 WP_146411066.1 radical SAM family heme chaperone HemW -
  Dpoa569_RS03615 (Dpoa569_0000731) rdgB 818394..818987 (-) 594 WP_042872608.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  Dpoa569_RS03620 (Dpoa569_0000732) yggU 819035..819325 (-) 291 WP_042872605.1 DUF167 family protein YggU -
  Dpoa569_RS03625 (Dpoa569_0000733) - 819322..819876 (-) 555 WP_042872602.1 YggT family protein -
  Dpoa569_RS03630 (Dpoa569_0000734) proC 819919..820740 (-) 822 WP_042872600.1 pyrroline-5-carboxylate reductase -
  Dpoa569_RS03635 (Dpoa569_0000735) - 820774..821511 (-) 738 WP_322619422.1 YggS family pyridoxal phosphate-dependent enzyme -
  Dpoa569_RS03640 (Dpoa569_0000736) pilT 821534..822556 (+) 1023 WP_042872598.1 type IV pilus twitching motility protein PilT Machinery gene
  Dpoa569_RS03645 (Dpoa569_0000737) - 822596..824668 (-) 2073 WP_227983122.1 bifunctional diguanylate cyclase/phosphodiesterase -
  Dpoa569_RS03650 (Dpoa569_0000738) codA 825140..826407 (-) 1268 Protein_701 cytosine deaminase -
  Dpoa569_RS03655 (Dpoa569_0000739) - 826404..827345 (-) 942 WP_042872596.1 CdaR family transcriptional regulator -
  Dpoa569_RS03660 - 827385..827543 (+) 159 WP_155683829.1 hypothetical protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37014.17 Da        Isoelectric Point: 6.1174

>NTDB_id=376093 Dpoa569_RS03640 WP_042872598.1 821534..822556(+) (pilT) [Dickeya poaceiphila strain NCPPB 569]
MDVSEWVSASVKQNVSDLHLCSGHPPVWRIDGQLVTDVAQPPLHNAELEQWCRSWLDPACDQQLRQYGQTDCALTLPEGQ
RLRANLFMQRHGLSAALRLIPQHQPDLQVLGAPPVIAGLVTCTDGLILVTGATGSGKSTTLAAMIAELNQRRACHIITLE
DPIEFLHTSTRCLIQQREMGRHSEGFASALRAALREDPDVLLLGELRDSETIRLALTAAETGHLVLATLHTRRAVQAVDR
LVDVFPAEEKAFVRAQLAASLQAVVAQKLVPGVQGGRVALFEVLTATPAVSNLIREGKTHQLTSVLQTGSQVGMQTFEQS
YQQRYREGVLPDDAPDGASS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=376093 Dpoa569_RS03640 WP_042872598.1 821534..822556(+) (pilT) [Dickeya poaceiphila strain NCPPB 569]
ATGGATGTGAGTGAATGGGTGAGCGCTAGTGTAAAACAGAATGTATCCGATCTGCACCTGTGTAGCGGTCACCCGCCGGT
GTGGCGTATCGACGGTCAACTGGTGACGGATGTCGCCCAGCCGCCGTTGCACAATGCTGAGCTGGAGCAGTGGTGCCGCA
GTTGGCTGGACCCGGCCTGCGATCAGCAACTGCGCCAATACGGGCAGACCGACTGTGCGCTGACGCTGCCGGAAGGTCAG
CGGTTACGGGCAAACCTGTTTATGCAGCGTCACGGTTTGTCGGCAGCACTACGCTTGATCCCGCAACATCAGCCTGATTT
GCAGGTGCTGGGTGCGCCGCCGGTGATAGCGGGTCTGGTCACCTGCACCGATGGCCTGATTCTGGTGACGGGGGCGACCG
GCAGCGGTAAATCCACCACGCTGGCAGCGATGATAGCCGAACTTAATCAACGCCGTGCTTGCCACATCATTACGCTGGAA
GACCCGATAGAGTTCCTGCATACCAGCACGCGCTGCCTGATCCAACAGCGGGAAATGGGCCGTCACAGCGAAGGGTTCGC
CAGTGCGTTGCGAGCCGCGTTGCGTGAAGACCCGGACGTGCTGTTGCTCGGTGAGCTGCGCGACAGCGAGACGATTCGAC
TGGCGCTGACGGCGGCGGAAACCGGTCACCTGGTACTGGCTACGCTGCATACCCGGCGGGCTGTACAGGCGGTGGATCGG
TTAGTCGATGTGTTCCCTGCCGAGGAGAAAGCGTTTGTGCGTGCGCAACTGGCTGCGAGTTTGCAGGCGGTGGTGGCGCA
GAAATTAGTACCGGGGGTGCAAGGCGGACGCGTGGCACTGTTTGAGGTACTGACTGCTACGCCCGCGGTCAGCAACCTGA
TCCGCGAAGGCAAAACTCACCAGTTGACCAGTGTGCTGCAAACCGGCAGTCAGGTGGGGATGCAAACCTTTGAGCAGAGC
TATCAGCAGCGGTATCGGGAAGGCGTGTTGCCGGATGATGCGCCTGACGGCGCATCATCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8I2U2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.246

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

97.059

0.482
  pilT Vibrio cholerae strain A1552

49.697

97.059

0.482
  pilT Neisseria gonorrhoeae MS11

47.953

100

0.482
  pilT Acinetobacter baumannii D1279779

48.348

97.941

0.474
  pilT Acinetobacter nosocomialis M2

48.348

97.941

0.474
  pilT Acinetobacter baumannii strain A118

48.348

97.941

0.474
  pilT Acinetobacter baylyi ADP1

48.348

97.941

0.474
  pilT Pseudomonas stutzeri DSM 10701

47.605

98.235

0.468
  pilT Pseudomonas aeruginosa PAK

47.879

97.059

0.465
  pilT Legionella pneumophila strain Lp02

46.667

97.059

0.453
  pilT Legionella pneumophila strain ERS1305867

46.667

97.059

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.216

98.235

0.415
  pilU Vibrio cholerae strain A1552

38.012

100

0.382
  pilU Pseudomonas stutzeri DSM 10701

37.69

96.765

0.365

Multiple sequence alignment