Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GC56T3_RS04690 Genome accession   NC_014206
Coordinates   927472..928527 (+) Length   351 a.a.
NCBI ID   WP_013144602.1    Uniprot ID   -
Organism   Geobacillus sp. C56-T3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 922472..933527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GC56T3_RS04675 (GC56T3_0860) - 923632..924411 (+) 780 WP_013144599.1 PRC-barrel domain-containing protein -
  GC56T3_RS04680 (GC56T3_0861) - 924428..925798 (+) 1371 WP_013144600.1 VanW family protein -
  GC56T3_RS04685 (GC56T3_0862) - 925795..927459 (+) 1665 WP_013144601.1 GspE/PulE family protein -
  GC56T3_RS04690 (GC56T3_0863) pilT 927472..928527 (+) 1056 WP_013144602.1 type IV pilus twitching motility protein PilT Machinery gene
  GC56T3_RS04695 (GC56T3_0864) - 928514..929725 (+) 1212 WP_013144603.1 type II secretion system F family protein -
  GC56T3_RS04700 (GC56T3_0865) - 929811..930338 (+) 528 WP_013144604.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GC56T3_RS04705 (GC56T3_0866) - 930340..931086 (+) 747 WP_013144605.1 A24 family peptidase -
  GC56T3_RS04710 (GC56T3_0867) pilM 931108..932046 (+) 939 WP_013144606.1 type IV pilus biogenesis protein PilM -
  GC56T3_RS04715 (GC56T3_0868) - 932036..932611 (+) 576 WP_013144607.1 fimbrial protein -
  GC56T3_RS04720 (GC56T3_0869) - 932608..933327 (+) 720 WP_013144608.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38495.51 Da        Isoelectric Point: 6.7270

>NTDB_id=37339 GC56T3_RS04690 WP_013144602.1 927472..928527(+) (pilT) [Geobacillus sp. C56-T3]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVNGELRAYGHERLHPEETEQMAKAVIPAPLWQQFQETGELDFSYGIAGV
SRFRLNVFKQRSCVSLAVRLIPVRIPTLEELGLPDVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREVGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTASAPAAIE
RMIDVFPPAQQAQIRLQLASVLVAIIAQRLFPNRHKTGRTVALEILVNNAAVANLIRNEKEHQIINIMQTSRSIGMQTME
MSVKELAADGRIDPLAAEVYLAGERGDDGAV

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=37339 GC56T3_RS04690 WP_013144602.1 927472..928527(+) (pilT) [Geobacillus sp. C56-T3]
ATGAAGGAAAAGCTTGAGGCGTTATTGCGCGCGGCCTTTGAATGCCGCGCTTCCGACGTGCATTTGACCGTCGGTGCGCC
GCCGATTTTTCGCGTGAACGGGGAGTTGAGAGCTTACGGCCATGAGCGCCTGCACCCGGAGGAAACAGAGCAAATGGCGA
AAGCCGTCATTCCGGCGCCGCTTTGGCAGCAGTTTCAGGAAACCGGAGAGCTTGATTTTTCGTATGGCATTGCCGGCGTT
TCCCGCTTTCGCTTAAACGTGTTTAAACAGCGGTCATGCGTGTCGCTCGCCGTGCGCCTCATCCCGGTGCGCATCCCGAC
GCTGGAGGAGCTTGGCCTGCCGGATGTCTTAAAGCGCATCGCCGAAAAGCCGCACGGACTCGTGCTTGTCACCGGACCGA
CCGGCAGCGGCAAATCGACGACATTGGCGGCGATGATCGACTATATGAACAAAACGATGTCGAAACATATCATCACGCTG
GAAGATCCAATCGAGTATTTGCATAAACACGGGAAATCGATCATCGACCAACGCGAGGTGGGATTCGACACTGGCAGTTT
TGCCGCCGGATTGCGCGCTGCGCTTCGTCAAGATCCAGACGTCATTTTAGTCGGGGAAATGCGCGATTTGGAAACAATCC
AAACGGCAATCACCGCCGCGGAAACGGGCCATTTAGTGCTTGGCACGCTTCATACGGCGAGCGCGCCGGCGGCGATTGAG
CGGATGATTGACGTCTTTCCGCCGGCCCAGCAGGCGCAAATCCGGCTGCAGCTTGCTTCCGTGCTCGTTGCCATCATCGC
TCAGCGCTTGTTTCCAAACAGACACAAAACCGGGCGGACGGTAGCGCTGGAAATTTTAGTGAACAATGCGGCCGTCGCCA
ACTTAATCCGCAACGAAAAAGAACATCAAATCATCAACATTATGCAAACGAGCCGCAGCATCGGCATGCAGACGATGGAA
ATGAGCGTTAAGGAGCTTGCAGCCGATGGACGCATCGATCCGCTGGCGGCTGAAGTGTATTTGGCGGGGGAGCGTGGGGA
CGATGGCGCAGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.994

89.174

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.773

92.877

0.453
  pilT Vibrio cholerae strain A1552

48.773

92.877

0.453
  pilT Neisseria gonorrhoeae MS11

47.879

94.017

0.45
  pilT Neisseria meningitidis 8013

47.879

94.017

0.45
  pilT Pseudomonas aeruginosa PAK

48.607

92.023

0.447
  pilT Acinetobacter baylyi ADP1

47.853

92.877

0.444
  pilT Acinetobacter baumannii strain A118

46.97

94.017

0.442
  pilT Acinetobacter baumannii D1279779

46.97

94.017

0.442
  pilT Acinetobacter nosocomialis M2

46.667

94.017

0.439
  pilT Pseudomonas stutzeri DSM 10701

47.368

92.023

0.436
  pilT Legionella pneumophila strain Lp02

47.649

90.883

0.433
  pilT Legionella pneumophila strain ERS1305867

47.649

90.883

0.433
  pilU Vibrio cholerae strain A1552

41.127

100

0.416
  pilU Pseudomonas stutzeri DSM 10701

43.465

93.732

0.407

Multiple sequence alignment