Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   FEO94_RS16685 Genome accession   NZ_CP040433
Coordinates   3633814..3634242 (-) Length   142 a.a.
NCBI ID   WP_072167642.1    Uniprot ID   A0A246I4G3
Organism   Stenotrophomonas maltophilia strain SKK55     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3628814..3639242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEO94_RS16670 (FEO94_16820) pilR 3629344..3630717 (+) 1374 WP_075676419.1 sigma-54 dependent transcriptional regulator Regulator
  FEO94_RS16675 (FEO94_16825) pilB 3630891..3632621 (-) 1731 WP_154351295.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FEO94_RS16680 (FEO94_16830) - 3632781..3633614 (-) 834 WP_185807474.1 sterol desaturase family protein -
  FEO94_RS16685 (FEO94_16835) pilA/pilAI 3633814..3634242 (-) 429 WP_072167642.1 pilin Machinery gene
  FEO94_RS16690 (FEO94_16840) pilC 3634597..3635856 (+) 1260 WP_154351297.1 type II secretion system F family protein Machinery gene
  FEO94_RS16695 (FEO94_16845) - 3635860..3636723 (+) 864 WP_065185263.1 A24 family peptidase -
  FEO94_RS16700 (FEO94_16850) coaE 3636735..3637346 (+) 612 WP_154351298.1 dephospho-CoA kinase -
  FEO94_RS16705 (FEO94_16855) - 3637520..3637906 (-) 387 WP_053499205.1 hypothetical protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14668.68 Da        Isoelectric Point: 8.4832

>NTDB_id=364404 FEO94_RS16685 WP_072167642.1 3633814..3634242(-) (pilA/pilAI) [Stenotrophomonas maltophilia strain SKK55]
MKNQKGFTLIELMIVVAIIAILAAIAIPAYQDYVVRSQAASALAEITPGKVGFEQATNEGKTPSTTATDAGFIGVAASTS
YCNVAVTATTIACTTKNGNATKFNSKNITWTRDANSGLWSCSTSLDAKYRPGQCGAAAAPTP

Nucleotide


Download         Length: 429 bp        

>NTDB_id=364404 FEO94_RS16685 WP_072167642.1 3633814..3634242(-) (pilA/pilAI) [Stenotrophomonas maltophilia strain SKK55]
ATGAAGAACCAGAAGGGCTTCACCCTTATCGAACTGATGATCGTCGTTGCGATCATCGCCATTTTGGCTGCGATTGCGAT
CCCGGCCTATCAGGACTACGTCGTTCGTTCGCAGGCGGCTTCCGCCCTGGCTGAGATTACCCCCGGCAAGGTCGGCTTCG
AACAGGCGACCAACGAAGGCAAGACTCCGTCGACCACCGCAACCGATGCGGGCTTCATCGGCGTTGCTGCCAGCACCTCC
TACTGCAATGTGGCCGTGACTGCCACGACCATTGCCTGCACCACCAAGAACGGCAACGCTACCAAGTTCAACAGCAAGAA
CATTACGTGGACCCGCGACGCCAACAGCGGCCTGTGGTCCTGCTCCACCAGCCTCGATGCGAAGTATCGTCCGGGCCAGT
GTGGTGCTGCCGCCGCTCCGACGCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A246I4G3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

68.657

94.366

0.648
  pilA/pilAII Pseudomonas stutzeri DSM 10701

60.902

93.662

0.57
  pilA Acinetobacter baumannii strain A118

46.98

100

0.493
  pilA Pseudomonas aeruginosa PAK

45.946

100

0.479
  comP Acinetobacter baylyi ADP1

44.521

100

0.458
  pilA Vibrio cholerae strain A1552

44.828

100

0.458
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.828

100

0.458
  pilA Vibrio cholerae C6706

44.828

100

0.458
  pilA Ralstonia pseudosolanacearum GMI1000

38.272

100

0.437
  pilA2 Legionella pneumophila str. Paris

43.478

97.183

0.423
  pilA2 Legionella pneumophila strain ERS1305867

43.478

97.183

0.423
  pilE Neisseria gonorrhoeae MS11

40

91.549

0.366

Multiple sequence alignment