Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FEO94_RS16675 Genome accession   NZ_CP040433
Coordinates   3630891..3632621 (-) Length   576 a.a.
NCBI ID   WP_154351295.1    Uniprot ID   A0AAX1ICP4
Organism   Stenotrophomonas maltophilia strain SKK55     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3625891..3637621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEO94_RS16660 (FEO94_16810) sucC 3626093..3627262 (-) 1170 WP_005418614.1 ADP-forming succinate--CoA ligase subunit beta -
  FEO94_RS16665 (FEO94_16815) - 3627670..3629283 (+) 1614 WP_134968485.1 ATP-binding protein -
  FEO94_RS16670 (FEO94_16820) pilR 3629344..3630717 (+) 1374 WP_075676419.1 sigma-54 dependent transcriptional regulator Regulator
  FEO94_RS16675 (FEO94_16825) pilB 3630891..3632621 (-) 1731 WP_154351295.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FEO94_RS16680 (FEO94_16830) - 3632781..3633614 (-) 834 WP_185807474.1 sterol desaturase family protein -
  FEO94_RS16685 (FEO94_16835) pilA/pilAI 3633814..3634242 (-) 429 WP_072167642.1 pilin Machinery gene
  FEO94_RS16690 (FEO94_16840) pilC 3634597..3635856 (+) 1260 WP_154351297.1 type II secretion system F family protein Machinery gene
  FEO94_RS16695 (FEO94_16845) - 3635860..3636723 (+) 864 WP_065185263.1 A24 family peptidase -
  FEO94_RS16700 (FEO94_16850) coaE 3636735..3637346 (+) 612 WP_154351298.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62225.14 Da        Isoelectric Point: 5.8733

>NTDB_id=364403 FEO94_RS16675 WP_154351295.1 3630891..3632621(-) (pilB) [Stenotrophomonas maltophilia strain SKK55]
MNTVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGIGNLDVSGGDEDISGGGDAGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPANLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=364403 FEO94_RS16675 WP_154351295.1 3630891..3632621(-) (pilB) [Stenotrophomonas maltophilia strain SKK55]
ATGAACACCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCTTCCGCCCGCGACGCCATGGCCAAGGCCGCTGCCGCCCGCCAGCCGCTGCCGACCTGGTTTGCGCAGAACAAGGTGG
TCGGGGCGGCCCAGTTGGCGGCGGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAAGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTGGGTACCAGCAACCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAACCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTCGAGCAGTGGCATGCCAGCCATGACACGCTGGGCGACGCGCTCGGTGGTGAC
GACGATGGCATTGGTAACCTGGATGTGTCCGGCGGTGACGAGGACATTTCCGGGGGCGGCGACGCCGGCATCGATGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGCGCCTCGGACATCCACT
TCGAGCCCTATGAAGACGACTACCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTGAAG
TTGAACCAGCGCATTGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAACCGGACCAGCAGAAGCTGTTC
CTGGAGGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCCGCGGCACTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTTGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCT
CGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGGCTGTGCAACAACTGCAAGCGCCCGGCCAATCTGCCCGAGCAT
GCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGACGCGGGTATCGAGCTGTACGAGGCGGTCGGCTGCGATGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGTTGGCTG
GCGGCAATGCGTTGCAGATTGCCGAGGCCGCGCAGCAGATCGGGGTGAACGATCTGCGCCAGTCGGCGTTGAAGAAGGCT
GCTGCGGGCATTACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.611

0.55
  pilB Acinetobacter baylyi ADP1

54.974

99.479

0.547
  pilB Legionella pneumophila strain ERS1305867

52.098

99.306

0.517
  pilF Neisseria gonorrhoeae MS11

49.12

98.611

0.484
  pilB Vibrio cholerae strain A1552

48.427

99.306

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

44.965

100

0.45
  pilB Vibrio campbellii strain DS40M4

45.343

98.785

0.448

Multiple sequence alignment