Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   SMI_RS01065 Genome accession   NC_013853
Coordinates   205584..206525 (+) Length   313 a.a.
NCBI ID   WP_000249543.1    Uniprot ID   D3H6J8
Organism   Streptococcus mitis B6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 200584..211525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMI_RS01045 (smi_0201) - 201295..202461 (-) 1167 WP_000163009.1 IS30-like element ISSmi1 family transposase -
  SMI_RS01050 (smi_0202) nagA 202683..203834 (+) 1152 WP_001134545.1 N-acetylglucosamine-6-phosphate deacetylase -
  SMI_RS01055 (smi_0203) - 203998..205056 (+) 1059 WP_000649461.1 zinc-dependent alcohol dehydrogenase family protein -
  SMI_RS01060 (smi_0204) - 205142..205507 (+) 366 WP_000286392.1 DUF1033 family protein -
  SMI_RS01065 (smi_0205) comGA/cglA/cilD 205584..206525 (+) 942 WP_000249543.1 competence type IV pilus ATPase ComGA Machinery gene
  SMI_RS01070 (smi_0206) comGB/cglB 206473..207489 (+) 1017 WP_164925538.1 competence type IV pilus assembly protein ComGB Machinery gene
  SMI_RS01075 (smi_0207) comGC/cglC 207491..207814 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  SMI_RS01080 (smi_0208) comGD/cglD 207777..208211 (+) 435 WP_164925504.1 competence type IV pilus minor pilin ComGD Machinery gene
  SMI_RS01085 (smi_0209) comGE/cglE 208174..208476 (+) 303 WP_153197443.1 competence type IV pilus minor pilin ComGE Machinery gene
  SMI_RS01090 (smi_0210) comGF/cglF 208439..208900 (+) 462 WP_000250522.1 competence type IV pilus minor pilin ComGF Machinery gene
  SMI_RS01095 (smi_0211) comGG/cglG 208878..209291 (+) 414 WP_000265641.1 competence type IV pilus minor pilin ComGG Machinery gene
  SMI_RS01100 (smi_0212) - 209324..209909 (+) 586 Protein_201 class I SAM-dependent methyltransferase -
  SMI_RS01105 (smi_0213) comYH 209970..210923 (+) 954 WP_000345096.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35607.48 Da        Isoelectric Point: 5.2801

>NTDB_id=36283 SMI_RS01065 WP_000249543.1 205584..206525(+) (comGA/cglA/cilD) [Streptococcus mitis B6]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYE
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLLEDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=36283 SMI_RS01065 WP_000249543.1 205584..206525(+) (comGA/cglA/cilD) [Streptococcus mitis B6]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATCGGTTGTTATGATTTTGAAAAATTTGCGGCCG
TAATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTTGGTTCCTGTGATTATGAA
TATGACCAGAAGATGGCGTCCCTACGTTTGTCTACTGTAGGCGATTATCGAGGGCATGAGAGTTTAGTTATTCGCCTGTT
GCATGATGAGGAGCAGGATTTGCATTTTTGGTTTCAGGATATTGAAGAACTGGGTAAGCAGTACAGGCAACGTGGTCTCT
ATCTCTTTGCTGGTCCAGTCGGAAGTGGCAAGACGACCTTGATGCACGAATTAGCCAAGTCTCTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTCCAGTTGCAGTTGAATGAGGCGATTGGGCTAAC
CTATGAAAATCTTATCAAGCTTTCCTTACGTCATCGTCCTGATCTCTTGATTATCGGAGAAATTCGGGACAGCGAGACGG
CGCGTGCAGTAGTTAGAGCTAGTTTGACAGGTGCGACCGTCTTTTCAACCATTCATGCCAAGAGTATTCGAGGTGTTTAT
GAGCGCTTGCTGGAGTTGGGTGTGAGTGAGGAGGAATTGGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATTGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAACAGATTGACCAGC
TTCTTGAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3H6J8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae D39

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae R6

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

96.166

100

0.962
  comYA Streptococcus gordonii str. Challis substr. CH1

79.032

99.042

0.783
  comYA Streptococcus mutans UA159

65.595

99.361

0.652
  comYA Streptococcus mutans UA140

65.595

99.361

0.652
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

55.769

99.681

0.556
  comGA Latilactobacillus sakei subsp. sakei 23K

43.019

84.665

0.364

Multiple sequence alignment