Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SMI_RS01070 Genome accession   NC_013853
Coordinates   206473..207489 (+) Length   338 a.a.
NCBI ID   WP_164925538.1    Uniprot ID   -
Organism   Streptococcus mitis B6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 201473..212489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMI_RS01050 (smi_0202) nagA 202683..203834 (+) 1152 WP_001134545.1 N-acetylglucosamine-6-phosphate deacetylase -
  SMI_RS01055 (smi_0203) - 203998..205056 (+) 1059 WP_000649461.1 zinc-dependent alcohol dehydrogenase family protein -
  SMI_RS01060 (smi_0204) - 205142..205507 (+) 366 WP_000286392.1 DUF1033 family protein -
  SMI_RS01065 (smi_0205) comGA/cglA/cilD 205584..206525 (+) 942 WP_000249543.1 competence type IV pilus ATPase ComGA Machinery gene
  SMI_RS01070 (smi_0206) comGB/cglB 206473..207489 (+) 1017 WP_164925538.1 competence type IV pilus assembly protein ComGB Machinery gene
  SMI_RS01075 (smi_0207) comGC/cglC 207491..207814 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  SMI_RS01080 (smi_0208) comGD/cglD 207777..208211 (+) 435 WP_164925504.1 competence type IV pilus minor pilin ComGD Machinery gene
  SMI_RS01085 (smi_0209) comGE/cglE 208174..208476 (+) 303 WP_153197443.1 competence type IV pilus minor pilin ComGE Machinery gene
  SMI_RS01090 (smi_0210) comGF/cglF 208439..208900 (+) 462 WP_000250522.1 competence type IV pilus minor pilin ComGF Machinery gene
  SMI_RS01095 (smi_0211) comGG/cglG 208878..209291 (+) 414 WP_000265641.1 competence type IV pilus minor pilin ComGG Machinery gene
  SMI_RS01100 (smi_0212) - 209324..209909 (+) 586 Protein_201 class I SAM-dependent methyltransferase -
  SMI_RS01105 (smi_0213) comYH 209970..210923 (+) 954 WP_000345096.1 class I SAM-dependent methyltransferase Machinery gene
  SMI_RS01110 (smi_0214) - 211034..212167 (+) 1134 WP_001113734.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38467.43 Da        Isoelectric Point: 9.4587

>NTDB_id=36284 SMI_RS01070 WP_164925538.1 206473..207489(+) (comGB/cglB) [Streptococcus mitis B6]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLISAVFLLALTFYKRSSKMRVFSILARLPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDLAQTLQNGQEFSQTIGTYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=36284 SMI_RS01070 WP_164925538.1 206473..207489(+) (comGB/cglB) [Streptococcus mitis B6]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTGGTTGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGCG
TGACCCAGATGCGCACAGGTTTGTCTCAGGGGAAATCATTTTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGCGCCATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAACTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGCTTTCTTCTCTTAATTATGTTGG
GACTCCGTAACTATCTATTACCACAACTGGATAGTAGTAATATTGCCACGCAAATTATCGGAAATCTACCACAAATCTTT
CTAGGAATGGTAGGACTTATTTCAGCAGTTTTTCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGCGTGTCTT
TTCGATCTTAGCACGCCTCCCCTTCCTTGGAATTTTTGTACAGACCTATTTGACAGCTTATTACGCGCGTGAATGGGGCA
ATATGATTTCTCAAGGAATGGAGCTGACGCAGATTTTTCAAATCATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATTTGGCTCAAACTCTACAAAATGGTCAGGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAAGAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCTAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAATCGCACCATGAATCTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

97.041

100

0.97
  comGB/cglB Streptococcus mitis NCTC 12261

96.746

100

0.967
  comGB/cglB Streptococcus pneumoniae Rx1

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae D39

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae R6

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae TIGR4

95.858

100

0.959
  comYB Streptococcus gordonii str. Challis substr. CH1

72.647

100

0.731
  comYB Streptococcus mutans UA140

58.491

94.083

0.55
  comYB Streptococcus mutans UA159

58.491

94.083

0.55
  comGB Lactococcus lactis subsp. cremoris KW2

51.497

98.817

0.509

Multiple sequence alignment