Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   FCN25_RS11020 Genome accession   NZ_CP039928
Coordinates   2474410..2474838 (-) Length   142 a.a.
NCBI ID   WP_137224702.1    Uniprot ID   -
Organism   Shewanella sp. MEBiC00475     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2469410..2479838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FCN25_RS11000 coaE 2469644..2470273 (-) 630 WP_137224694.1 dephospho-CoA kinase -
  FCN25_RS11005 pilD 2470270..2471196 (-) 927 WP_137224696.1 A24 family peptidase Machinery gene
  FCN25_RS11010 pilC 2471222..2472490 (-) 1269 WP_137224698.1 type II secretion system F family protein Machinery gene
  FCN25_RS11015 pilB 2472598..2474304 (-) 1707 WP_137224700.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FCN25_RS11020 pilA/pilAI 2474410..2474838 (-) 429 WP_137224702.1 pilin Machinery gene
  FCN25_RS11025 nadC 2475801..2476679 (-) 879 WP_137224704.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FCN25_RS11030 ampD 2476888..2477442 (+) 555 WP_137224706.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FCN25_RS11035 ampE 2477601..2478452 (+) 852 WP_137224708.1 beta-lactamase regulator AmpE -
  FCN25_RS11040 pdhR 2478763..2479515 (+) 753 WP_137224710.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14595.93 Da        Isoelectric Point: 9.1886

>NTDB_id=361012 FCN25_RS11020 WP_137224702.1 2474410..2474838(-) (pilA/pilAI) [Shewanella sp. MEBiC00475]
MKGINQIKNAKGFTLIELMIVVAIIGILAAIALPAYQDYTVKSQAGSALSEVNSVKTQFEIVTNEGKAPSLVATEVGYIG
QTATGAAYCGLAVTATTIVCTTKGGNATAFNGKTITLTRTAATGVWACTSNLDAKFKPGKCT

Nucleotide


Download         Length: 429 bp        

>NTDB_id=361012 FCN25_RS11020 WP_137224702.1 2474410..2474838(-) (pilA/pilAI) [Shewanella sp. MEBiC00475]
ATGAAAGGTATCAACCAAATCAAAAACGCTAAGGGTTTTACTCTTATCGAATTAATGATCGTAGTCGCGATCATCGGTAT
TCTTGCAGCAATCGCATTGCCTGCATATCAGGATTACACAGTTAAGTCCCAAGCAGGCTCAGCTTTGTCTGAAGTGAATT
CTGTCAAAACACAATTTGAAATAGTAACTAATGAAGGTAAAGCACCTAGCTTAGTCGCAACTGAGGTTGGTTATATTGGA
CAAACTGCAACAGGTGCTGCTTATTGTGGTTTAGCAGTAACGGCAACGACTATTGTTTGTACAACAAAAGGTGGTAATGC
TACTGCCTTTAATGGAAAAACAATTACTTTAACTCGTACTGCTGCCACAGGAGTATGGGCTTGTACGTCTAACTTAGATG
CTAAGTTCAAACCAGGTAAGTGTACCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

62.963

95.07

0.599
  pilA/pilAII Pseudomonas stutzeri DSM 10701

56.618

95.775

0.542
  pilA Pseudomonas aeruginosa PAK

43.709

100

0.465
  pilA Acinetobacter baumannii strain A118

48.175

96.479

0.465
  comP Acinetobacter baylyi ADP1

43.448

100

0.444
  pilA2 Legionella pneumophila strain ERS1305867

43.066

96.479

0.415
  pilA2 Legionella pneumophila str. Paris

43.066

96.479

0.415
  pilA/pilA1 Eikenella corrodens VA1

42.857

93.662

0.401
  pilA Vibrio cholerae C6706

38.095

100

0.394
  pilA Vibrio cholerae strain A1552

38.095

100

0.394
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.095

100

0.394
  pilA Vibrio parahaemolyticus RIMD 2210633

41.538

91.549

0.38

Multiple sequence alignment