Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FCN25_RS11015 Genome accession   NZ_CP039928
Coordinates   2472598..2474304 (-) Length   568 a.a.
NCBI ID   WP_137224700.1    Uniprot ID   -
Organism   Shewanella sp. MEBiC00475     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2467598..2479304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FCN25_RS10985 mutT 2468252..2468650 (-) 399 WP_137224688.1 8-oxo-dGTP diphosphatase MutT -
  FCN25_RS10990 yacG 2468662..2468874 (-) 213 WP_137224690.1 DNA gyrase inhibitor YacG -
  FCN25_RS10995 zapD 2468917..2469651 (-) 735 WP_137224692.1 cell division protein ZapD -
  FCN25_RS11000 coaE 2469644..2470273 (-) 630 WP_137224694.1 dephospho-CoA kinase -
  FCN25_RS11005 pilD 2470270..2471196 (-) 927 WP_137224696.1 A24 family peptidase Machinery gene
  FCN25_RS11010 pilC 2471222..2472490 (-) 1269 WP_137224698.1 type II secretion system F family protein Machinery gene
  FCN25_RS11015 pilB 2472598..2474304 (-) 1707 WP_137224700.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FCN25_RS11020 pilA/pilAI 2474410..2474838 (-) 429 WP_137224702.1 pilin Machinery gene
  FCN25_RS11025 nadC 2475801..2476679 (-) 879 WP_137224704.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FCN25_RS11030 ampD 2476888..2477442 (+) 555 WP_137224706.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FCN25_RS11035 ampE 2477601..2478452 (+) 852 WP_137224708.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62733.27 Da        Isoelectric Point: 5.3588

>NTDB_id=361011 FCN25_RS11015 WP_137224700.1 2472598..2474304(-) (pilB) [Shewanella sp. MEBiC00475]
MSTAGLNLGLSTLFIKKGLLTEEEVATSISQSRKTKVSLVTTVIQAKLLSAREIAELCYEEYGTPLLDLNEFDIVSISEE
LLNKKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSLGLHAEAILVEDDKLLKALEKIIEDDISGLDLAGVDEEA
LANMEVTDNERRDEPTGDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSNRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSSLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLLYEEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPACKHEEIIPVAELQRLGFA
EADIAKGFTVFKPVGCELCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATLAKQQGMRDLRQSGLLKVIHGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=361011 FCN25_RS11015 WP_137224700.1 2472598..2474304(-) (pilB) [Shewanella sp. MEBiC00475]
ATGTCGACTGCAGGATTAAATCTTGGCCTTTCAACTTTATTTATCAAAAAAGGTCTACTAACAGAAGAAGAAGTCGCAAC
GTCAATCTCGCAATCTCGTAAAACAAAAGTTTCACTCGTCACTACTGTAATTCAAGCAAAACTTCTGTCTGCTCGTGAAA
TTGCAGAATTATGTTATGAAGAATACGGGACACCTCTCCTAGATTTAAACGAATTTGATATTGTCTCTATTTCAGAAGAA
TTATTAAACAAAAAACTCATTGAAAAGCACAAATGTTTACCACTTTTTAAAAGGGGTAATCGCCTTTATATTGCCACCTC
AGATCCGACAAATATAGCCGCTCTTGAAGATTTTCAATTTAGTTTAGGTTTGCATGCGGAAGCTATTCTCGTTGAGGATG
ATAAGCTATTAAAAGCACTTGAAAAAATCATTGAAGATGATATTTCAGGATTAGACTTAGCCGGTGTGGACGAAGAAGCG
CTTGCCAATATGGAAGTCACTGATAATGAAAGGCGGGATGAACCAACGGGTGACAGCAGTGATGATGCCCCAATTGTTAT
TTATATCAATAAAATCCTCACCGATGCCATTCGTAAAGGTGCCTCTGACTTACATTTTGAGCCCTATGAAAAACGCTACC
GGATCCGTTTCCGTATCGATGGAATATTACACGAAGTATCTGAGCCACCAGTCAACCTATCCAACCGGATTTCAGCCCGC
TTAAAGGTCATGTCAAAGCTGGATATTGCTGAGCGCAGAGTGCCACAAGATGGCCGCATTAAAATGAAACTGTCGCGCAC
CAAATCTATCGATTTTCGTGTCAGTAGTTTGCCCACCATTTGGGGCGAAAAAATCGTAATGCGTATTTTAGACTCATCAT
CCGCACAACTCGGTATTGAAAAGCTCGGATATGAAGATGATCAACGTTTACTCTATGAAGAAATGCTCGCTAAACCCCAA
GGGATGATTTTAGTTACCGGCCCTACAGGTTCGGGTAAAACAGTCTCCCTGTATACTGGGCTGAATATTCTTAATACCGA
AGAGCGTAATATCTCTACTGCAGAAGACCCGGTTGAAATTAACCTCGAAGGGGTTAATCAGGTCCATATTAATTTAAAAG
CAGGTTTAACCTTTGCTTCTGCACTACGTTCTTTTTTACGTCAGGATCCTGATGTGGTGATGGTAGGTGAAATTCGTGAT
TTAGAAACCGCCGAAATCGCCATTAAAGCCGCGCAAACAGGTCATTTAGTGTTATCAACGCTGCACACCAACTCTGCAGC
AGAAACCCTCACTCGTTTAATCAACATGGGGGTGCCTGGTTATAACATTGCCAGTTCCGTTAACTTAATCATTGCTCAAC
GACTTGCCAGACGCTTATGCCCAGCATGTAAACATGAAGAAATAATCCCAGTGGCAGAACTACAACGTTTAGGATTTGCT
GAAGCTGACATAGCCAAAGGCTTTACCGTATTTAAACCTGTAGGCTGTGAGCTATGCTCTGGCGGTTATAAAGGCCGAGT
CGGCATTTACGAAGTAATGAAAATGAGTGATGAAATTGCCCGTACTATTATGGAAGGGGGTAACTCACTGCAAATAGCAA
CCCTCGCTAAGCAGCAAGGTATGCGTGATTTACGTCAATCTGGGTTGCTCAAAGTCATCCACGGTGTAACCAGTATCGCG
GAAATTAACCGTGTCACTAGCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.576

98.768

0.569
  pilB Acinetobacter baumannii D1279779

59.225

95.423

0.565
  pilB Legionella pneumophila strain ERS1305867

56.762

98.944

0.562
  pilB Vibrio cholerae strain A1552

54.167

97.183

0.526
  pilB Vibrio parahaemolyticus RIMD 2210633

51.494

100

0.516
  pilB Vibrio campbellii strain DS40M4

50.174

100

0.509
  pilF Neisseria gonorrhoeae MS11

46.996

99.648

0.468
  pilF Thermus thermophilus HB27

38.503

98.768

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.786

98.592

0.363

Multiple sequence alignment