Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FBF68_RS10580 Genome accession   NZ_CP039452
Coordinates   2176170..2177150 (+) Length   326 a.a.
NCBI ID   WP_041908325.1    Uniprot ID   A0AAU7N8L0
Organism   Enterobacter bugandensis strain 1367     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2171170..2182150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBF68_RS10545 (FAI37_10545) - 2171533..2171859 (+) 327 WP_028014375.1 YggL family protein -
  FBF68_RS10550 (FAI37_10550) - 2171917..2172633 (+) 717 WP_041908321.1 DUF2884 domain-containing protein -
  FBF68_RS10555 (FAI37_10555) hemW 2172838..2173980 (-) 1143 WP_063155145.1 radical SAM family heme chaperone HemW -
  FBF68_RS10560 (FAI37_10560) - 2173973..2174566 (-) 594 WP_010435473.1 XTP/dITP diphosphatase -
  FBF68_RS10565 (FAI37_10565) yggU 2174570..2174866 (-) 297 WP_047364545.1 DUF167 family protein YggU -
  FBF68_RS10570 (FAI37_10570) - 2174863..2175429 (-) 567 WP_045260768.1 YggT family protein -
  FBF68_RS10575 (FAI37_10575) - 2175451..2176152 (-) 702 WP_063155144.1 YggS family pyridoxal phosphate-dependent enzyme -
  FBF68_RS10580 (FAI37_10580) pilT 2176170..2177150 (+) 981 WP_041908325.1 type IV pilus twitching motility protein PilT Machinery gene
  FBF68_RS10585 (FAI37_10585) ruvX 2177161..2177577 (-) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  FBF68_RS10590 (FAI37_10590) - 2177577..2178137 (-) 561 WP_028014368.1 YqgE/AlgH family protein -
  FBF68_RS10595 (FAI37_10595) gshB 2178212..2179159 (-) 948 WP_033146605.1 glutathione synthase -
  FBF68_RS10600 (FAI37_10600) rsmE 2179179..2179910 (-) 732 WP_063155143.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FBF68_RS10605 (FAI37_10605) endA 2179962..2180669 (-) 708 WP_028014365.1 deoxyribonuclease I -
  FBF68_RS10610 (FAI37_10610) - 2180764..2181261 (-) 498 WP_028014364.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35583.65 Da        Isoelectric Point: 6.3904

>NTDB_id=358713 FBF68_RS10580 WP_041908325.1 2176170..2177150(+) (pilT) [Enterobacter bugandensis strain 1367]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNRHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=358713 FBF68_RS10580 WP_041908325.1 2176170..2177150(+) (pilT) [Enterobacter bugandensis strain 1367]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCGGATGTCGGGGCGTTGCTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCCGTTACCCTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCACTTGG
GGTACCGCGCGCTATTCCCGAACTGTTGTCCAGCGACAACGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCAATGGTCGACTTCCTCAACCGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGGGCAGCACAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTACGAGCTGCTGGTAAACACCGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
AGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCCGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain Lp02

49.689

98.773

0.491
  pilT Legionella pneumophila strain ERS1305867

49.689

98.773

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39

Multiple sequence alignment