Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E6B08_RS28505 Genome accession   NZ_CP039371
Coordinates   6170145..6171143 (-) Length   332 a.a.
NCBI ID   WP_136917027.1    Uniprot ID   A0A4D6XKB9
Organism   Pseudomonas putida strain 1290     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6165145..6176143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E6B08_RS28485 (E6B08_28485) argS 6166338..6168074 (+) 1737 WP_136917023.1 arginine--tRNA ligase -
  E6B08_RS28490 (E6B08_28490) - 6168076..6168777 (+) 702 WP_136917024.1 SPOR domain-containing protein -
  E6B08_RS28495 (E6B08_28495) - 6168872..6169288 (-) 417 WP_136917025.1 NINE protein -
  E6B08_RS28500 (E6B08_28500) - 6169454..6170080 (+) 627 WP_136917026.1 C40 family peptidase -
  E6B08_RS28505 (E6B08_28505) pilT 6170145..6171143 (-) 999 WP_136917027.1 type IV pilus twitching motility protein PilT Machinery gene
  E6B08_RS28510 (E6B08_28510) - 6171209..6171895 (+) 687 WP_136917028.1 YggS family pyridoxal phosphate-dependent enzyme -
  E6B08_RS28515 (E6B08_28515) proC 6171934..6172752 (+) 819 WP_136917029.1 pyrroline-5-carboxylate reductase -
  E6B08_RS28520 (E6B08_28520) - 6172763..6173353 (+) 591 WP_136917030.1 YggT family protein -
  E6B08_RS28525 (E6B08_28525) metX 6173452..6174591 (+) 1140 WP_136917031.1 homoserine O-succinyltransferase MetX -
  E6B08_RS28530 (E6B08_28530) metW 6174599..6175219 (+) 621 WP_136917032.1 methionine biosynthesis protein MetW -
  E6B08_RS28535 (E6B08_28535) - 6175242..6175676 (+) 435 WP_136917033.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36059.92 Da        Isoelectric Point: 6.5058

>NTDB_id=358232 E6B08_RS28505 WP_136917027.1 6170145..6171143(-) (pilT) [Pseudomonas putida strain 1290]
MDVTDLLALAVEAGASDLHLAVGEIPMLRCEGELRRLELPSLKPAMLDDALAQWLDPGQQQRWLQGEDLDLALELPILGR
FRVNLYRQLRGAAATMRLVPNAIASLAELDLEEVYRAVAGYTDGLILVGGPTGSGKSSTLAALLQQLGRQRALHIVTLED
PIEVIHKAERSLVTQREIGRHCRDFSQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAASSLDRL
VEVFGAEEKPLVRAMLADSLRMVVAQVLVKRVGGGRVAAREVLMATPAVRNLIREGRMAQLCSVMQAGGAEGMRTMEGAL
RELKAKGSIGDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=358232 E6B08_RS28505 WP_136917027.1 6170145..6171143(-) (pilT) [Pseudomonas putida strain 1290]
ATGGATGTGACCGACCTGTTGGCCCTGGCTGTGGAAGCAGGCGCGAGTGACCTGCACCTGGCCGTGGGCGAGATACCGAT
GCTGCGCTGCGAGGGCGAGCTGCGACGCCTGGAGCTGCCGAGCCTGAAGCCGGCGATGCTGGACGATGCCTTGGCGCAAT
GGCTGGACCCTGGCCAGCAGCAGCGCTGGCTGCAAGGCGAGGACCTGGACCTGGCATTGGAGCTGCCGATACTCGGGCGT
TTTCGCGTGAACCTGTACCGCCAGTTGCGCGGTGCGGCGGCCACGATGCGCCTGGTGCCGAATGCCATTGCCAGCCTCGC
CGAACTCGACCTGGAAGAAGTGTATCGAGCTGTTGCCGGATACACCGATGGCCTGATCCTGGTCGGCGGCCCGACCGGCA
GCGGCAAATCCAGCACTCTGGCTGCACTGCTGCAGCAGCTCGGTCGCCAGCGTGCGCTGCATATCGTCACGCTGGAAGAC
CCCATCGAAGTTATCCACAAGGCTGAGCGCAGTCTGGTGACTCAGCGGGAAATCGGCCGTCACTGCCGGGACTTTTCCCA
GGGTCTGCGCAGTGCCCTGCGCCAGGATCCGGATGTGATCATGATTGGCGAACTGCGGGACCTGGAGACCATACGCCTGG
CGTTGCGGGCGGCGGAGACCGGGCATCTGGTGCTGGCCACGGTGCACACGCGGTCGGCAGCCAGCAGCCTCGACCGCCTG
GTGGAAGTGTTCGGCGCCGAAGAAAAGCCCCTGGTGCGGGCGATGCTGGCCGACTCACTGCGCATGGTGGTGGCGCAGGT
GCTGGTCAAGCGTGTCGGTGGCGGACGTGTGGCAGCCAGGGAGGTGCTGATGGCGACGCCGGCGGTGCGCAACCTGATTC
GCGAGGGGCGCATGGCGCAGCTGTGTTCGGTGATGCAGGCAGGGGGTGCCGAGGGGATGCGGACGATGGAGGGAGCGTTG
CGGGAGCTGAAGGCGAAAGGTTCGATCGGTGACCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4D6XKB9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.456

99.096

0.5
  pilT Pseudomonas stutzeri DSM 10701

50.152

99.096

0.497
  pilT Acinetobacter baylyi ADP1

49.697

99.398

0.494
  pilT Vibrio cholerae strain A1552

48.936

99.096

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.936

99.096

0.485
  pilT Acinetobacter baumannii D1279779

48.485

99.398

0.482
  pilT Acinetobacter baumannii strain A118

48.485

99.398

0.482
  pilT Acinetobacter nosocomialis M2

48.182

99.398

0.479
  pilT Legionella pneumophila strain Lp02

47.879

99.398

0.476
  pilT Legionella pneumophila strain ERS1305867

47.879

99.398

0.476
  pilT Neisseria meningitidis 8013

47.432

99.699

0.473
  pilT Neisseria gonorrhoeae MS11

47.13

99.699

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.925

96.687

0.425
  pilU Vibrio cholerae strain A1552

35.693

100

0.364
  pilU Pseudomonas stutzeri DSM 10701

36.364

99.398

0.361

Multiple sequence alignment