Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   E5790_RS14415 Genome accession   NZ_CP039255
Coordinates   3094274..3094702 (+) Length   142 a.a.
NCBI ID   WP_049416952.1    Uniprot ID   -
Organism   Stenotrophomonas sp. PAMC25021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3089274..3099702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5790_RS14390 (E5790_14390) - 3089881..3090195 (+) 315 WP_198282880.1 hypothetical protein -
  E5790_RS14400 (E5790_14400) coaE 3091170..3091781 (-) 612 WP_049440374.1 dephospho-CoA kinase -
  E5790_RS14405 (E5790_14405) - 3091793..3092656 (-) 864 WP_005418626.1 A24 family peptidase -
  E5790_RS14410 (E5790_14410) pilC 3092660..3093919 (-) 1260 WP_049416954.1 type II secretion system F family protein Machinery gene
  E5790_RS14415 (E5790_14415) pilA/pilAI 3094274..3094702 (+) 429 WP_049416952.1 pilin Machinery gene
  E5790_RS14420 (E5790_14420) - 3094906..3095739 (+) 834 WP_168708240.1 sterol desaturase family protein -
  E5790_RS14425 (E5790_14425) pilB 3095900..3097630 (+) 1731 WP_111206599.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E5790_RS14430 (E5790_14430) pilR 3097745..3099118 (-) 1374 WP_049405671.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14638.66 Da        Isoelectric Point: 8.4832

>NTDB_id=357675 E5790_RS14415 WP_049416952.1 3094274..3094702(+) (pilA/pilAI) [Stenotrophomonas sp. PAMC25021]
MKNQKGFTLIELMIVVAIIAILAAIAIPAYQDYVVRSQAASALAEITPGKVGFEQATNEGKTPSTTATDAGFIGVAASTS
YCNVAVTATTIACTTKNGNATKFNSKNITWTRDANSGLWSCSTSLDAKYRPGQCGAAAAPAP

Nucleotide


Download         Length: 429 bp        

>NTDB_id=357675 E5790_RS14415 WP_049416952.1 3094274..3094702(+) (pilA/pilAI) [Stenotrophomonas sp. PAMC25021]
ATGAAGAACCAGAAGGGCTTCACCCTTATCGAACTGATGATCGTTGTTGCGATCATCGCCATTTTGGCTGCGATTGCGAT
CCCGGCCTATCAGGACTACGTCGTTCGTTCGCAGGCGGCTTCCGCCCTGGCTGAGATTACCCCCGGCAAGGTCGGCTTCG
AACAGGCGACCAACGAAGGCAAGACTCCGTCGACCACCGCAACCGATGCGGGCTTCATCGGCGTTGCTGCCAGCACCTCC
TACTGCAATGTGGCCGTGACTGCCACGACCATTGCCTGCACCACTAAGAACGGCAACGCTACCAAGTTCAACAGCAAGAA
CATTACGTGGACCCGCGACGCCAACAGCGGCCTGTGGTCCTGCTCCACCAGCCTCGACGCGAAGTATCGTCCGGGCCAGT
GTGGTGCCGCCGCCGCTCCGGCGCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

68.657

94.366

0.648
  pilA/pilAII Pseudomonas stutzeri DSM 10701

60.902

93.662

0.57
  pilA Acinetobacter baumannii strain A118

46.897

100

0.479
  pilA Pseudomonas aeruginosa PAK

45.27

100

0.472
  comP Acinetobacter baylyi ADP1

44.521

100

0.458
  pilA Vibrio cholerae strain A1552

44.828

100

0.458
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.828

100

0.458
  pilA Vibrio cholerae C6706

44.828

100

0.458
  pilA Ralstonia pseudosolanacearum GMI1000

38.272

100

0.437
  pilA2 Legionella pneumophila str. Paris

43.478

97.183

0.423
  pilA2 Legionella pneumophila strain ERS1305867

43.478

97.183

0.423
  pilE Neisseria gonorrhoeae MS11

34.731

100

0.408

Multiple sequence alignment