Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E5790_RS14425 Genome accession   NZ_CP039255
Coordinates   3095900..3097630 (+) Length   576 a.a.
NCBI ID   WP_111206599.1    Uniprot ID   -
Organism   Stenotrophomonas sp. PAMC25021     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3090900..3102630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5790_RS14400 (E5790_14400) coaE 3091170..3091781 (-) 612 WP_049440374.1 dephospho-CoA kinase -
  E5790_RS14405 (E5790_14405) - 3091793..3092656 (-) 864 WP_005418626.1 A24 family peptidase -
  E5790_RS14410 (E5790_14410) pilC 3092660..3093919 (-) 1260 WP_049416954.1 type II secretion system F family protein Machinery gene
  E5790_RS14415 (E5790_14415) pilA/pilAI 3094274..3094702 (+) 429 WP_049416952.1 pilin Machinery gene
  E5790_RS14420 (E5790_14420) - 3094906..3095739 (+) 834 WP_168708240.1 sterol desaturase family protein -
  E5790_RS14425 (E5790_14425) pilB 3095900..3097630 (+) 1731 WP_111206599.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E5790_RS14430 (E5790_14430) pilR 3097745..3099118 (-) 1374 WP_049405671.1 sigma-54 dependent transcriptional regulator Regulator
  E5790_RS14435 (E5790_14435) - 3099179..3100792 (-) 1614 WP_049440369.1 ATP-binding protein -
  E5790_RS14440 (E5790_14440) sucC 3101198..3102367 (+) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62257.14 Da        Isoelectric Point: 5.8722

>NTDB_id=357676 E5790_RS14425 WP_111206599.1 3095900..3097630(+) (pilB) [Stenotrophomonas sp. PAMC25021]
MNTVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALSGD
DDGIGNLDVSGGDEDISGAGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPANLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=357676 E5790_RS14425 WP_111206599.1 3095900..3097630(+) (pilB) [Stenotrophomonas sp. PAMC25021]
ATGAACACCGTAACCACCGCCAACCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCTTCCGCCCGCGACGCCATGGCCAAGGCCGCCGCGGCCCGCCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TCGGGGCGGCCCAGTTGGCGGCGGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAACCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTCGAGCAGTGGCATGCCAGCCATGACACGCTGGGCGACGCGCTGAGCGGTGAC
GACGATGGCATTGGCAACCTGGATGTGTCCGGCGGTGACGAAGACATTTCCGGAGCCGGCGACTCCGGCATCGATGCCAA
GGGTGATGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGCGCCTCGGACATCCACT
TCGAGCCCTATGAAGACGACTATCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTGAAG
CTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGACGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATAC
CGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGTCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCACTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCACACCAACGATGCACCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCT
CGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGGTTGTGCAACAACTGCAAGCGCCCGGCCAACCTGCCAGAGCAC
GCGCTGCTGGCTGAAGGCTTTACCCAGGCGCAGCTGGATGCGGGTATCGAGCTGTACGAGGCGGTCGGCTGTGACGAGTG
CACCGAGGGGTACAAGGGCCGCACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGCTGGCCG
GTGGCAATGCGTTGCAGATTGCCGAGGCCGCACAGCAGATCGGGGTGAACGATCTGCGCCAGTCGGCGTTGAAGAAGGCG
GCTGCGGGTGTCACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.611

0.552
  pilB Acinetobacter baylyi ADP1

54.974

99.479

0.547
  pilB Legionella pneumophila strain ERS1305867

51.923

99.306

0.516
  pilB Vibrio cholerae strain A1552

48.427

99.306

0.481
  pilF Neisseria gonorrhoeae MS11

48.768

98.611

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

44.792

100

0.448
  pilB Vibrio campbellii strain DS40M4

45.009

99.132

0.446

Multiple sequence alignment