Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   E5790_RS14410 Genome accession   NZ_CP039255
Coordinates   3092660..3093919 (-) Length   419 a.a.
NCBI ID   WP_049416954.1    Uniprot ID   A0A246I4D3
Organism   Stenotrophomonas sp. PAMC25021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3087660..3098919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5790_RS14385 (E5790_14385) - 3088672..3089316 (+) 645 WP_126411298.1 hypothetical protein -
  E5790_RS14390 (E5790_14390) - 3089881..3090195 (+) 315 WP_198282880.1 hypothetical protein -
  E5790_RS14400 (E5790_14400) coaE 3091170..3091781 (-) 612 WP_049440374.1 dephospho-CoA kinase -
  E5790_RS14405 (E5790_14405) - 3091793..3092656 (-) 864 WP_005418626.1 A24 family peptidase -
  E5790_RS14410 (E5790_14410) pilC 3092660..3093919 (-) 1260 WP_049416954.1 type II secretion system F family protein Machinery gene
  E5790_RS14415 (E5790_14415) pilA/pilAI 3094274..3094702 (+) 429 WP_049416952.1 pilin Machinery gene
  E5790_RS14420 (E5790_14420) - 3094906..3095739 (+) 834 WP_168708240.1 sterol desaturase family protein -
  E5790_RS14425 (E5790_14425) pilB 3095900..3097630 (+) 1731 WP_111206599.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45918.24 Da        Isoelectric Point: 10.1940

>NTDB_id=357674 E5790_RS14410 WP_049416954.1 3092660..3093919(-) (pilC) [Stenotrophomonas sp. PAMC25021]
MSVSGSAMKKEPVARSTTELQPFVWEGTDKRGVKMKGEQMAKNANLLRAELRRQGITPKAVKAKPKPIFGSTGSRITPKD
IAFFSRQMATMMKSGVPIVSSLEIIGEGHKNLRMKRMIGTIRADIEGGSSLYEAISRHPVQFDELYRNLVRAGEGAGVLE
TVLDTVANYKENIETLKGKIKKALFYPIMVMAVAILVSGILLVWVVPQFEDVFKGFGAELPAFTQLIVGVSRFLVSWWWL
ILFTFVGAIVGFIAAYKRSPKLQHTMDRLILRVPVIGQIMHNSSIARFARTTAVTFKAGVPLVEALGIVAGATGNKVYEE
SVLRMRDDVSVGYPVNMAMKQLNLFPHMVIQMTGIGEEAGALDAMLFKVAEYYEQEVNNAVDALSSLLEPFIMVIIGGIV
GSIVIGMYLPIFKLAGAVG

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=357674 E5790_RS14410 WP_049416954.1 3092660..3093919(-) (pilC) [Stenotrophomonas sp. PAMC25021]
ATGTCTGTCAGTGGCAGTGCAATGAAGAAAGAACCCGTGGCGCGCAGCACCACGGAACTGCAGCCGTTTGTATGGGAGGG
GACCGACAAGCGGGGTGTGAAGATGAAAGGCGAGCAGATGGCAAAGAACGCCAATCTGTTGCGCGCAGAATTGAGACGCC
AGGGCATCACGCCCAAAGCGGTGAAGGCGAAGCCAAAGCCTATTTTCGGCAGCACAGGCAGCAGGATTACGCCGAAGGAC
ATTGCCTTCTTCAGTCGCCAGATGGCAACCATGATGAAGTCAGGTGTGCCTATCGTCTCTTCACTCGAGATCATTGGCGA
AGGGCACAAGAACCTTCGGATGAAGAGGATGATCGGAACGATCCGCGCCGATATCGAGGGCGGCTCATCCCTATATGAGG
CGATCAGCAGACATCCAGTGCAGTTCGACGAGCTGTATCGCAACCTGGTCCGTGCGGGCGAAGGCGCTGGTGTATTGGAG
ACCGTGCTTGACACTGTGGCCAACTACAAGGAGAACATCGAAACACTCAAGGGCAAGATCAAGAAGGCGCTGTTCTATCC
GATCATGGTCATGGCAGTCGCCATCCTGGTCAGTGGCATCCTACTTGTCTGGGTGGTCCCTCAATTCGAAGATGTCTTCA
AAGGCTTCGGAGCCGAGTTGCCCGCTTTCACCCAGCTGATTGTTGGCGTTTCTCGCTTCTTGGTGTCTTGGTGGTGGCTG
ATCCTGTTCACTTTTGTGGGGGCGATAGTTGGCTTCATCGCAGCCTACAAGCGCTCTCCGAAGCTGCAACACACGATGGA
CCGATTGATCCTGAGGGTGCCAGTGATCGGCCAGATCATGCACAACAGCTCAATTGCCCGCTTTGCACGTACCACTGCAG
TTACCTTCAAGGCCGGCGTGCCCTTGGTGGAGGCGTTGGGCATTGTCGCCGGCGCGACCGGCAACAAGGTCTACGAAGAG
TCCGTACTGCGGATGCGTGACGACGTGTCGGTCGGCTATCCCGTGAACATGGCCATGAAGCAGCTGAACCTATTTCCTCA
TATGGTCATCCAGATGACCGGCATCGGCGAGGAGGCCGGTGCTCTGGACGCCATGCTGTTCAAGGTCGCCGAGTACTATG
AGCAGGAAGTCAACAACGCCGTGGACGCATTGAGCAGTCTGCTTGAACCTTTCATCATGGTCATCATTGGCGGAATTGTC
GGCAGCATCGTGATCGGCATGTACCTGCCGATCTTCAAGCTCGCAGGAGCGGTAGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(288-410)

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A246I4D3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

50.99

96.42

0.492
  pilC Pseudomonas stutzeri DSM 10701

51.768

94.511

0.489
  pilC Acinetobacter baylyi ADP1

49.261

96.897

0.477
  pilC Acinetobacter baumannii D1279779

48.866

94.749

0.463
  pilG Neisseria gonorrhoeae MS11

43.467

94.988

0.413
  pilG Neisseria meningitidis 44/76-A

42.965

94.988

0.408
  pilC Vibrio cholerae strain A1552

39.655

96.897

0.384
  pilC Vibrio campbellii strain DS40M4

38.653

95.704

0.37
  pilC Thermus thermophilus HB27

37.346

97.136

0.363

Multiple sequence alignment