Detailed information NTG0035292

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: DTOX_RS24920
• Genome accession: NC_013216
• Coordinates: 3439659..3440150 (-)
• Length: 163 a.a.
• NCBI ID: WP_278184559.1
• Uniprot ID: -
• Organism: Desulfofarcimen acetoxidans DSM 771
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3434659..3445150

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DTOX_RS15690 (Dtox_3234)	-	3434738..3435928 (+)	1191	WP_157862983.1	VanW family protein	-
DTOX_RS15695 (Dtox_3236)	-	3436263..3436523 (+)	261	WP_015758667.1	aspartyl-phosphate phosphatase Spo0E family protein	-
DTOX_RS15700 (Dtox_3238)	-	3437106..3437600 (+)	495	WP_015758668.1	cation:proton antiporter regulatory subunit	-
DTOX_RS15705 (Dtox_3239)	-	3437603..3438820 (+)	1218	WP_015758669.1	cation:proton antiporter	-
DTOX_RS15710 (Dtox_3242)	-	3439261..3439512 (+)	252	WP_015758670.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
DTOX_RS24920	pilT	3439659..3440150 (-)	492	WP_278184559.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
DTOX_RS24925	-	3440180..3440674 (-)	495	WP_278184560.1	ATPase, T2SS/T4P/T4SS family	-
DTOX_RS15720 (Dtox_3244)	-	3441166..3442236 (-)	1071	WP_015758671.1	type IV pilus twitching motility protein PilT	-
DTOX_RS15725 (Dtox_3245)	-	3442313..3443926 (-)	1614	WP_015758672.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
DTOX_RS15730 (Dtox_3246)	-	3443928..3444365 (-)	438	WP_015758673.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Sequence

Protein

• Length: 163 a.a.
• Molecular weight: 18368.53 Da
• Isoelectric Point: 10.7237

>NTDB_id=35292 DTOX_RS24920 WP_278184559.1 3439659..3440150(-) (pilT) [Desulfofarcimen acetoxidans DSM 771]
MRAALRQDPDVILVGEMRDLETISIAVTAAETGHLILATLHTMNAPQTVSRIIDAFDSVQQKQIRMQLSNSLVGIISQRL
LPRIDGRGWLAAMEILVATPAVRNLIRENKLHQIFSYLQAGSKFGMQTMDSHLQTRHLKHKLYLVKGGPISLPIWFNCTF
RKN

Nucleotide

• Length: 492 bp

>NTDB_id=35292 DTOX_RS24920 WP_278184559.1 3439659..3440150(-) (pilT) [Desulfofarcimen acetoxidans DSM 771]
TTGCGGGCAGCGCTCAGGCAGGATCCTGATGTTATTCTGGTGGGAGAAATGAGGGATTTGGAAACTATCTCTATTGCAGT
CACCGCGGCAGAGACCGGTCATCTGATCCTGGCCACTTTGCACACCATGAATGCGCCCCAAACTGTCAGCCGGATTATTG
ATGCTTTTGATTCGGTTCAGCAGAAGCAAATCCGTATGCAGCTGTCCAATTCTTTAGTAGGCATCATCTCTCAGAGACTG
CTGCCTCGCATTGACGGCAGGGGATGGCTGGCAGCCATGGAAATACTGGTGGCCACACCGGCGGTACGCAACCTGATCAG
AGAAAACAAGCTCCATCAGATTTTTTCTTATTTGCAGGCGGGTTCCAAATTTGGCATGCAGACAATGGATAGCCACCTGC
AAACCAGGCATTTGAAACATAAGCTTTATCTGGTCAAGGGGGGCCCAATTAGCCTTCCTATATGGTTTAACTGCACATTC
AGAAAAAACTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (1-82)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	58.503	90.184	0.528
pilT	Neisseria meningitidis 8013	60.741	82.822	0.503
pilT	Neisseria gonorrhoeae MS11	60	82.822	0.497
pilT	Acinetobacter baylyi ADP1	50.98	93.865	0.479
pilT	Legionella pneumophila strain ERS1305867	49.032	95.092	0.466
pilT	Legionella pneumophila strain Lp02	49.032	95.092	0.466
pilT	Pseudomonas stutzeri DSM 10701	55.224	82.209	0.454
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	56.923	79.755	0.454
pilT	Vibrio cholerae strain A1552	56.923	79.755	0.454
pilT	Acinetobacter baumannii D1279779	54.478	82.209	0.448
pilT	Pseudomonas aeruginosa PAK	54.478	82.209	0.448
pilT	Acinetobacter baumannii strain A118	54.478	82.209	0.448
pilT	Acinetobacter nosocomialis M2	54.478	82.209	0.448
pilU	Pseudomonas stutzeri DSM 10701	42.958	87.117	0.374
pilU	Acinetobacter baylyi ADP1	45.113	81.595	0.368