Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E2L09_RS12230 Genome accession   NZ_CP037955
Coordinates   2586665..2588035 (-) Length   456 a.a.
NCBI ID   WP_010720638.1    Uniprot ID   A0A1V8X781
Organism   Enterococcus hirae strain CQP3-9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2581665..2593035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2L09_RS12215 (E2L09_12245) epsC 2582945..2583481 (-) 537 WP_010720635.1 serine O-acetyltransferase EpsC -
  E2L09_RS12220 (E2L09_12250) gltX 2583794..2585254 (-) 1461 WP_010720636.1 glutamate--tRNA ligase -
  E2L09_RS12225 (E2L09_12255) - 2585471..2586631 (-) 1161 WP_160231918.1 PIN/TRAM domain-containing protein -
  E2L09_RS12230 (E2L09_12260) radA 2586665..2588035 (-) 1371 WP_010720638.1 DNA repair protein RadA Machinery gene
  E2L09_RS12235 (E2L09_12265) - 2588087..2588617 (-) 531 WP_053765952.1 dUTP diphosphatase -
  E2L09_RS12240 (E2L09_12270) proB 2588819..2589625 (+) 807 WP_010720640.1 glutamate 5-kinase -
  E2L09_RS12245 (E2L09_12275) - 2589622..2590866 (+) 1245 WP_034700060.1 glutamate-5-semialdehyde dehydrogenase -
  E2L09_RS12250 (E2L09_12280) - 2590970..2591314 (+) 345 WP_010720642.1 hypothetical protein -
  E2L09_RS12255 (E2L09_12285) - 2591403..2591720 (-) 318 WP_010737260.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49925.56 Da        Isoelectric Point: 7.3886

>NTDB_id=351005 E2L09_RS12230 WP_010720638.1 2586665..2588035(-) (radA) [Enterococcus hirae strain CQP3-9]
MAKKAKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIAEVIPKKEPRVKTKLDELN
RVLGGGVVPGSMILIGGDPGIGKSTLLLQVSQQLADIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEQISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLEGATGSAIVVAMEGTRPILVEIQALVTPTMYG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGGWNPPTGIEVVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=351005 E2L09_RS12230 WP_010720638.1 2586665..2588035(-) (radA) [Enterococcus hirae strain CQP3-9]
ATGGCAAAAAAAGCGAAAGTCCAGTTTGTTTGCCAAAATTGTGGCTATGTTTCGCCTAAATTCTTAGGGCGTTGCCCAAA
TTGCGGGAAATGGAACACGATGGTCGAGGAAATCGAGCAAGATACGACTGACCGTAGAACACGAACAAGTTTGACTGGAG
AAAAAGCCAAACCAACGAAAATTGCAGAAGTGATTCCTAAAAAGGAACCACGAGTCAAAACAAAATTAGATGAATTGAAT
CGAGTTTTGGGTGGGGGCGTAGTTCCGGGGTCAATGATTTTGATTGGTGGCGATCCAGGCATCGGTAAATCCACATTACT
TTTACAAGTATCCCAACAATTAGCTGATATCGGCGGGAAAGTATTATATGTTTCTGGAGAAGAAAGTGCAGAACAAATTA
AATTACGTGCCGAGCGTTTGGGCTCAATCAATACAGAATTTTACTTATATGCTGAAACTGATATGAATGAAATTAGCCGT
GCCATCGAACAGATTTCACCAGATTATGTGATCATTGATTCGATTCAAACGATGACACAACCTGATATTACGAGTGTAGC
AGGGAGTGTCAGTCAAGTGAGAGAAACGACTGCTGAGTTGTTGAAAATAGCGAAAACAAATGGGATTGCGATTTTTATCG
TAGGACATGTAACGAAAGAAGGCTCGATTGCGGGTCCACGTATGCTTGAACATATGGTTGATACTGTTTTGTATTTTGAA
GGAGAACAGCATCATAGTTTTCGTATTTTGAGAGCAGTAAAGAATCGATTTGGTTCAACAAATGAAATTGGTATATTTGA
AATGCATGAACATGGATTAGAAGAAGTTGCGAATCCTTCCCAAATCTTTTTGGAAGAACGATTAGAAGGAGCAACAGGCT
CAGCGATTGTTGTGGCGATGGAAGGTACCCGCCCAATTTTAGTGGAAATCCAAGCACTGGTAACACCAACAATGTATGGA
AATGCTAAACGAACCACTACTGGCTTAGATTTTAACCGAGTGTCATTGATCATGGCAGTTCTCGAAAAACGAGCAGGCTT
ACTGTTGCAGAATCAAGATGCTTACTTAAAAGCAGCAGGTGGGGTAAAATTAAATGAACCAGCCATTGATTTAGCGATTG
CTGTGAGTATTGCTTCCAGTTACAAAGAAAAAGGGACACAACCGACAGAATGTTTTATTGGCGAAATCGGGTTGACGGGC
GAAATTCGCCGTGTCAGTCATATTGAACAGCGTGTCAAAGAAGTACAAAAGCTGGGTTTCACAAAAGTTTATTTGCCAAA
GAACAATCTAGGAGGGTGGAATCCTCCGACTGGCATTGAAGTCGTAGGTGTCGCTACCTTGGCGGAAACGTTAAAACGTG
TCTTTCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V8X781

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

73.846

99.781

0.737
  radA Streptococcus pneumoniae D39

73.846

99.781

0.737
  radA Streptococcus pneumoniae R6

73.846

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

67.406

98.904

0.667

Multiple sequence alignment