Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E1B22_RS07025 Genome accession   NZ_CP037895
Coordinates   1675438..1676472 (+) Length   344 a.a.
NCBI ID   WP_135225085.1    Uniprot ID   A0A4P7GTV6
Organism   Thermaerobacter sp. FW80     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1670438..1681472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1B22_RS07005 (E1B22_07005) - 1671835..1672686 (+) 852 WP_135225082.1 NAD-dependent deacylase -
  E1B22_RS07010 (E1B22_07010) - 1672773..1673279 (+) 507 WP_243123223.1 DinB family protein -
  E1B22_RS07015 (E1B22_07015) - 1673538..1673795 (+) 258 WP_135225083.1 MoaD/ThiS family protein -
  E1B22_RS07020 (E1B22_07020) - 1673824..1675140 (+) 1317 WP_135225084.1 acetamidase/formamidase family protein -
  E1B22_RS07025 (E1B22_07025) pilT 1675438..1676472 (+) 1035 WP_135225085.1 type IV pilus twitching motility protein PilT Machinery gene
  E1B22_RS07030 (E1B22_07030) - 1676696..1677517 (+) 822 WP_135225086.1 A24 family peptidase -
  E1B22_RS13890 (E1B22_07035) - 1677776..1679752 (+) 1977 WP_135225087.1 GspE/PulE family protein -
  E1B22_RS07040 (E1B22_07040) - 1679749..1680975 (+) 1227 WP_135225088.1 type II secretion system F family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37250.96 Da        Isoelectric Point: 8.0019

>NTDB_id=350540 E1B22_RS07025 WP_135225085.1 1675438..1676472(+) (pilT) [Thermaerobacter sp. FW80]
MAGPELALWLRQAAECGASDLHLTVESPPVVRVHGELQDLPGPRLRPEHVAALVRPILDQRLQERLERDGQVDFAYSLPG
VGRFRANVYRQRGSLAAAFRLIPTSVPDLGELGLPPAVAGLCELSQGLVLVTGPTGSGKSTTLAAMVDRINRTRRLHIVT
LEDPIEYLHRHRRSLVNQREVGIDVPTFAAGLRAALREDPDVIMVGEMRDLDTIATALTAAETGHLVLATLHTPSAPQAV
DRIIDVFPAEQQNQVRVQLAGVLEAVIAQRLVPTADGRGRHVVAEVLLGTAAVRNLIREGKTAQLPAVMETSRQYGMVTL
RESAGQLLARGLVTREALLRAGVR

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=350540 E1B22_RS07025 WP_135225085.1 1675438..1676472(+) (pilT) [Thermaerobacter sp. FW80]
GTGGCAGGGCCGGAGCTGGCCCTCTGGTTGCGCCAGGCGGCCGAATGCGGCGCCTCCGATCTCCACCTGACGGTGGAGTC
GCCGCCGGTGGTGCGGGTCCACGGCGAGTTGCAGGACCTGCCGGGACCTCGGCTGCGACCGGAGCATGTCGCGGCGCTGG
TTCGTCCGATCCTGGACCAGCGGCTCCAGGAGCGGCTGGAGCGGGACGGGCAGGTGGACTTCGCCTACAGCCTGCCCGGC
GTCGGCCGCTTCCGGGCCAACGTCTACCGGCAGCGGGGCAGCCTGGCGGCCGCCTTCCGCCTGATCCCCACCTCCGTACC
CGATCTGGGCGAACTCGGCCTGCCGCCGGCCGTGGCCGGCCTCTGCGAGCTGTCCCAGGGGCTCGTGCTCGTGACCGGCC
CCACGGGCAGCGGGAAGTCGACCACCCTGGCGGCGATGGTCGACCGGATCAACCGCACCCGGCGCCTCCACATCGTGACG
TTGGAGGATCCCATCGAGTACCTGCACCGCCACCGCCGGAGCCTGGTGAACCAGCGGGAGGTCGGGATCGACGTCCCCAC
CTTCGCGGCCGGCCTGCGGGCCGCCCTGCGGGAAGACCCCGACGTGATCATGGTCGGGGAGATGCGGGACCTGGACACCA
TCGCCACGGCGTTGACGGCCGCGGAGACCGGGCACCTGGTGCTCGCCACCCTGCACACCCCCAGCGCTCCCCAGGCGGTG
GACCGGATCATCGACGTCTTCCCCGCGGAGCAGCAGAACCAGGTGCGGGTGCAGCTGGCGGGGGTCCTGGAGGCGGTGAT
CGCCCAGCGGCTGGTGCCCACCGCCGACGGGCGGGGTCGGCACGTCGTGGCCGAGGTCCTGCTGGGCACCGCGGCGGTGC
GCAACCTGATCCGCGAGGGCAAGACGGCCCAGCTCCCGGCGGTGATGGAGACCTCGCGGCAGTACGGGATGGTGACCCTG
CGGGAGAGCGCGGGGCAGCTGCTCGCCCGGGGGTTGGTGACGCGAGAGGCGCTGCTTCGCGCCGGGGTGCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7GTV6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.149

97.384

0.488
  pilT Acinetobacter baylyi ADP1

50.789

92.151

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.936

95.64

0.468
  pilT Vibrio cholerae strain A1552

48.936

95.64

0.468
  pilT Pseudomonas aeruginosa PAK

49.074

94.186

0.462
  pilT Neisseria gonorrhoeae MS11

48.318

95.058

0.459
  pilT Acinetobacter baumannii strain A118

49.068

93.605

0.459
  pilT Acinetobacter baumannii D1279779

49.068

93.605

0.459
  pilT Pseudomonas stutzeri DSM 10701

48.615

94.477

0.459
  pilT Acinetobacter nosocomialis M2

48.758

93.605

0.456
  pilT Neisseria meningitidis 8013

48.607

93.895

0.456
  pilT Legionella pneumophila strain ERS1305867

45.921

96.221

0.442
  pilT Legionella pneumophila strain Lp02

45.921

96.221

0.442
  pilU Pseudomonas stutzeri DSM 10701

41.716

98.256

0.41
  pilU Vibrio cholerae strain A1552

40.95

97.965

0.401

Multiple sequence alignment