Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   EZZ81_RS04795 Genome accession   NZ_CP036495
Coordinates   1006681..1007124 (+) Length   147 a.a.
NCBI ID   WP_029243837.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain Psy35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1001681..1012124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ81_RS04770 (EZZ81_04860) yacG 1001835..1002044 (-) 210 WP_004887444.1 DNA gyrase inhibitor YacG -
  EZZ81_RS04775 (EZZ81_04865) coaE 1002041..1002664 (-) 624 WP_025993968.1 dephospho-CoA kinase -
  EZZ81_RS04780 (EZZ81_04870) pilD 1002661..1003533 (-) 873 WP_029243834.1 A24 family peptidase Machinery gene
  EZZ81_RS04785 (EZZ81_04875) pilC 1003535..1004752 (-) 1218 WP_029243835.1 type II secretion system F family protein Machinery gene
  EZZ81_RS04790 (EZZ81_04880) pilB 1004755..1006455 (-) 1701 WP_029243836.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EZZ81_RS04795 (EZZ81_04885) pilA2 1006681..1007124 (+) 444 WP_029243837.1 pilin Machinery gene
  EZZ81_RS04800 (EZZ81_04890) - 1007319..1007780 (+) 462 WP_029243838.1 hypothetical protein -
  EZZ81_RS04810 (EZZ81_04900) - 1007950..1008930 (-) 981 WP_029243839.1 YiiG family protein -
  EZZ81_RS04815 (EZZ81_04905) - 1008986..1009417 (-) 432 WP_029243840.1 hypothetical protein -
  EZZ81_RS04820 (EZZ81_04910) - 1009652..1010203 (-) 552 WP_029243841.1 DUF1523 family protein -
  EZZ81_RS04825 (EZZ81_04915) - 1010323..1011321 (+) 999 WP_029243842.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 14923.11 Da        Isoelectric Point: 9.0030

>NTDB_id=349144 EZZ81_RS04795 WP_029243837.1 1006681..1007124(+) (pilA2) [Pseudomonas viridiflava strain Psy35]
MKVQKTLQKGFTLIELMIVVAIVGILAAVAIPAYQDYTIRAQVAELVALTDGAKVAVSEAYQTTGAFPANNAAAGYGGGS
GTYTASVNIANGVITATAGGAAPVNNIIRGQTFVLTPLANGTNNTITWSCTGAASTIAAQYRPAICR

Nucleotide


Download         Length: 444 bp        

>NTDB_id=349144 EZZ81_RS04795 WP_029243837.1 1006681..1007124(+) (pilA2) [Pseudomonas viridiflava strain Psy35]
ATGAAAGTACAAAAAACGCTACAAAAAGGTTTTACGCTGATCGAACTGATGATCGTCGTAGCGATCGTGGGCATTCTCGC
AGCGGTGGCCATCCCTGCATATCAGGACTACACAATCCGTGCTCAGGTAGCAGAACTGGTTGCGCTCACGGATGGTGCGA
AGGTTGCTGTTAGTGAGGCTTATCAGACTACTGGTGCATTTCCCGCCAACAATGCTGCCGCTGGGTATGGCGGTGGGTCA
GGCACGTATACTGCGAGCGTGAACATCGCCAACGGTGTAATTACTGCGACTGCGGGTGGAGCAGCACCTGTCAATAATAT
TATTCGAGGCCAAACCTTTGTTTTGACCCCTCTAGCCAACGGCACTAACAATACAATCACTTGGTCCTGCACCGGTGCAG
CTAGCACTATCGCCGCTCAGTATCGACCAGCTATTTGTCGTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-30)

Pilin (bacterial filament) (Pilin)

(40-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

57.746

96.599

0.558
  pilA2 Legionella pneumophila strain ERS1305867

56.338

96.599

0.544
  pilA Ralstonia pseudosolanacearum GMI1000

42.775

100

0.503
  pilE Neisseria gonorrhoeae MS11

44.375

100

0.483
  pilE Neisseria gonorrhoeae strain FA1090

44.654

100

0.483
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.862

100

0.435
  pilA Vibrio campbellii strain DS40M4

40.385

100

0.429
  pilA/pilA1 Eikenella corrodens VA1

40.645

100

0.429
  comP Acinetobacter baylyi ADP1

41.611

100

0.422
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.333

100

0.422
  pilA Pseudomonas aeruginosa PAK

38.462

100

0.408
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.667

100

0.374

Multiple sequence alignment