Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EZZ81_RS04790 Genome accession   NZ_CP036495
Coordinates   1004755..1006455 (-) Length   566 a.a.
NCBI ID   WP_029243836.1    Uniprot ID   A0A1Y6JJZ8
Organism   Pseudomonas viridiflava strain Psy35     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 999755..1011455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ81_RS04760 (EZZ81_04850) - 1000251..1000880 (-) 630 WP_004884092.1 DUF1780 domain-containing protein -
  EZZ81_RS04765 (EZZ81_04855) - 1001005..1001466 (+) 462 WP_029243833.1 hypothetical protein -
  EZZ81_RS04770 (EZZ81_04860) yacG 1001835..1002044 (-) 210 WP_004887444.1 DNA gyrase inhibitor YacG -
  EZZ81_RS04775 (EZZ81_04865) coaE 1002041..1002664 (-) 624 WP_025993968.1 dephospho-CoA kinase -
  EZZ81_RS04780 (EZZ81_04870) pilD 1002661..1003533 (-) 873 WP_029243834.1 A24 family peptidase Machinery gene
  EZZ81_RS04785 (EZZ81_04875) pilC 1003535..1004752 (-) 1218 WP_029243835.1 type II secretion system F family protein Machinery gene
  EZZ81_RS04790 (EZZ81_04880) pilB 1004755..1006455 (-) 1701 WP_029243836.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EZZ81_RS04795 (EZZ81_04885) pilA2 1006681..1007124 (+) 444 WP_029243837.1 pilin Machinery gene
  EZZ81_RS04800 (EZZ81_04890) - 1007319..1007780 (+) 462 WP_029243838.1 hypothetical protein -
  EZZ81_RS04810 (EZZ81_04900) - 1007950..1008930 (-) 981 WP_029243839.1 YiiG family protein -
  EZZ81_RS04815 (EZZ81_04905) - 1008986..1009417 (-) 432 WP_029243840.1 hypothetical protein -
  EZZ81_RS04820 (EZZ81_04910) - 1009652..1010203 (-) 552 WP_029243841.1 DUF1523 family protein -
  EZZ81_RS04825 (EZZ81_04915) - 1010323..1011321 (+) 999 WP_029243842.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62585.77 Da        Isoelectric Point: 6.2562

>NTDB_id=349143 EZZ81_RS04790 WP_029243836.1 1004755..1006455(-) (pilB) [Pseudomonas viridiflava strain Psy35]
MSDVVVLTGLAKQLVVAELLSEKAAQQAFQQARRDKISLISYLVQNKLVKSLTLAEMASEQFGVPFMDISSLDKESQPKG
LVSEKLARQHHALPLWRRGNRLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTAAIDKFFDSDSGLGSMADIDLEL
DIEPGEGVKETSITSRDDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKVFRVRFRTDGILHEAAKPPINLASRIAARL
KVMASLDISERRKPQDGRVKLRVSKTKSIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKELYLEALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVNINISTAEDPVEINLEGVNQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKEVDVPHETLIKEGFPE
SKVGTFTLYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=349143 EZZ81_RS04790 WP_029243836.1 1004755..1006455(-) (pilB) [Pseudomonas viridiflava strain Psy35]
ATGAGTGATGTCGTTGTTCTTACCGGCCTGGCCAAGCAATTGGTCGTCGCCGAGCTTCTTTCCGAAAAAGCCGCTCAACA
GGCTTTCCAGCAGGCGCGACGCGACAAGATTTCGCTGATCAGCTATTTGGTTCAGAACAAACTGGTCAAGAGTCTCACGT
TGGCCGAAATGGCGTCCGAGCAGTTCGGCGTGCCATTCATGGACATATCAAGCCTCGATAAAGAAAGCCAGCCCAAGGGG
CTGGTAAGCGAAAAGCTCGCACGCCAACACCACGCCCTTCCCCTGTGGCGTCGGGGCAACAGACTGTTTATTGGCATCTC
GGACCCGACCAATCATCAGGCGGTGACCGACATCCAGTTCAGCACTGGTTTGAACACTGAAGCGATTCTGGTCGAAGACG
ACAAACTCACTGCAGCCATCGACAAGTTTTTTGACAGCGATAGCGGCCTCGGGTCCATGGCCGATATCGACCTGGAACTC
GATATCGAGCCGGGCGAAGGCGTCAAGGAAACATCCATCACCAGCCGCGACGATGCCGATGACGCTCCGGTAGTGCGTTT
CGTCAACAAAATGCTGCTGGATGCCATTCGCCTGGGCTCGTCCGATCTGCACTTCGAACCTTACGAAAAGGTGTTTCGCG
TGCGCTTTCGTACAGACGGGATTTTGCACGAGGCGGCGAAACCTCCTATCAATCTGGCCAGTCGTATCGCGGCGCGCCTG
AAGGTCATGGCCAGCCTGGACATCTCGGAACGGCGCAAGCCTCAGGACGGCCGGGTGAAGTTGCGTGTTTCCAAAACCAA
ATCCATCGACTTCCGGATGAACACGCTGCCGACGCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCGACCAGCG
CCCAGATGGGCATTGATGCTTTAGGCTACGAGCCGGATCAGAAAGAGCTTTATCTGGAAGCGCTCAAGCAGCCGCAGGGC
ATGATTCTGGTGACCGGCCCGACTGGCTCGGGCAAAACCGTTTCGCTGTACACGGGCCTTAATATTCTCAATACGGTGAA
TATCAATATTTCAACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCGTCAATCAGGTCAACGTCAACCCGCGTCAGG
GCATGGACTTCTCCCAGGCGTTGCGCGCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGTGAGATTCGTGACCTG
GAAACCGCCGAGATTGCGATCAAGGCTTCTCAGACCGGCCATATGGTGCTGTCCACATTGCACACCAACAGCGCGGCAGA
AACCCTGACTCGCCTGCATCACATGGGTGTTGCCGCATTCAACATCGCTACCGCGATCAACCTGATCATCGCCCAGCGAC
TGGCGCGCAAGCTGTGCAGTCATTGCAAGAAAGAAGTCGACGTCCCACACGAGACGCTAATCAAGGAAGGCTTTCCGGAG
TCGAAAGTCGGCACCTTCACACTGTACGGCCCGGTCGGATGCGAACACTGCAACGGCGGCTATCGAGGCCGAGTCGGGAT
TTACGAGGTCGTGAAAAAAACGCCGGAACTGGAACGCATCATCATGGAAGAAGGCAACTCGCTGGAGATTTCCAGGCAGA
TGCGCAAGGATGGTTTCAATGACCTGCGAACATCCGGCTTGTCGAAAGCCATGCAGGGCATTACCAGCCTGGAAGAAGTC
AATCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y6JJZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.406

99.293

0.56
  pilB Acinetobacter baylyi ADP1

56.228

99.293

0.558
  pilB Legionella pneumophila strain ERS1305867

54.561

100

0.549
  pilB Vibrio cholerae strain A1552

50.446

99.117

0.5
  pilB Vibrio campbellii strain DS40M4

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

49.186

97.703

0.481
  pilF Neisseria gonorrhoeae MS11

47.957

99.47

0.477
  pilF Thermus thermophilus HB27

38.732

100

0.389

Multiple sequence alignment