Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EZZ81_RS04785 Genome accession   NZ_CP036495
Coordinates   1003535..1004752 (-) Length   405 a.a.
NCBI ID   WP_029243835.1    Uniprot ID   A0AA46VTC3
Organism   Pseudomonas viridiflava strain Psy35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 998535..1009752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ81_RS04750 (EZZ81_04840) - 999522..999695 (-) 174 WP_004884089.1 DUF3094 family protein -
  EZZ81_RS04755 (EZZ81_04845) - 999709..1000254 (-) 546 WP_029243832.1 MOSC domain-containing protein -
  EZZ81_RS04760 (EZZ81_04850) - 1000251..1000880 (-) 630 WP_004884092.1 DUF1780 domain-containing protein -
  EZZ81_RS04765 (EZZ81_04855) - 1001005..1001466 (+) 462 WP_029243833.1 hypothetical protein -
  EZZ81_RS04770 (EZZ81_04860) yacG 1001835..1002044 (-) 210 WP_004887444.1 DNA gyrase inhibitor YacG -
  EZZ81_RS04775 (EZZ81_04865) coaE 1002041..1002664 (-) 624 WP_025993968.1 dephospho-CoA kinase -
  EZZ81_RS04780 (EZZ81_04870) pilD 1002661..1003533 (-) 873 WP_029243834.1 A24 family peptidase Machinery gene
  EZZ81_RS04785 (EZZ81_04875) pilC 1003535..1004752 (-) 1218 WP_029243835.1 type II secretion system F family protein Machinery gene
  EZZ81_RS04790 (EZZ81_04880) pilB 1004755..1006455 (-) 1701 WP_029243836.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EZZ81_RS04795 (EZZ81_04885) pilA2 1006681..1007124 (+) 444 WP_029243837.1 pilin Machinery gene
  EZZ81_RS04800 (EZZ81_04890) - 1007319..1007780 (+) 462 WP_029243838.1 hypothetical protein -
  EZZ81_RS04810 (EZZ81_04900) - 1007950..1008930 (-) 981 WP_029243839.1 YiiG family protein -
  EZZ81_RS04815 (EZZ81_04905) - 1008986..1009417 (-) 432 WP_029243840.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43934.74 Da        Isoelectric Point: 10.2256

>NTDB_id=349142 EZZ81_RS04785 WP_029243835.1 1003535..1004752(-) (pilC) [Pseudomonas viridiflava strain Psy35]
MASKAVKVSVFTWEGVDKKGSKISGELSGHNPALIKAQLRKQGVNPTKVRKKTVSIFGKGKKIKPLDIAFFARQMATMMK
AGVPLLQSFDIISEGAENPNMRSLVDSLKQEVSAGNSFATALRQKPDQFDNLFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPAAVLVVAFIVSGILLIKVVPQFQAVFAGFGAELPAFTRMVIGLSEVVQTWWLAIIGIFVGSFFIFK
RSYKQSQKFRDSVDRFLLKIPLIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVMKIKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMALLGVIVGGLVIAMYLPIFQ
LGGAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=349142 EZZ81_RS04785 WP_029243835.1 1003535..1004752(-) (pilC) [Pseudomonas viridiflava strain Psy35]
ATGGCCAGTAAAGCAGTCAAAGTCAGTGTTTTCACCTGGGAAGGCGTAGACAAGAAAGGCAGCAAGATCAGCGGCGAGTT
GAGTGGCCACAATCCGGCGCTGATCAAGGCTCAACTTCGTAAGCAGGGCGTCAACCCGACCAAAGTGCGGAAGAAGACAG
TCTCGATTTTCGGCAAGGGTAAAAAGATCAAGCCGCTGGACATCGCGTTTTTTGCCCGGCAGATGGCGACCATGATGAAA
GCGGGCGTTCCGCTGTTGCAGTCTTTCGACATTATCAGCGAGGGCGCGGAGAACCCGAACATGCGCTCGCTTGTGGACTC
TCTTAAACAGGAAGTATCCGCTGGTAATAGTTTTGCAACGGCGTTGAGGCAAAAGCCCGACCAATTCGACAATTTATTCT
GCAATCTGGTGGACGCAGGCGAACAGGCAGGTGCTCTTGAAAGCTTGTTGGACCGGGTAGCGACTTATAAGGAAAAAACC
GAAAAGCTCAAAGCCAAGATCAAGAAGGCCATGACTTATCCCGCCGCAGTTCTGGTTGTGGCGTTTATCGTCTCCGGGAT
TCTGCTCATCAAAGTGGTGCCACAATTTCAGGCGGTTTTTGCCGGTTTTGGGGCCGAGTTACCTGCTTTTACACGAATGG
TCATCGGTCTTTCCGAAGTGGTCCAAACCTGGTGGCTGGCCATTATCGGGATATTCGTCGGCAGCTTTTTCATATTCAAG
CGAAGCTACAAACAGTCACAGAAATTTCGCGACAGTGTTGACAGGTTTCTGTTGAAGATTCCGCTGATTGGTCCTTTGAT
CTTCAAGTCTTCAGTCGCTCGCTACGCTCGCACGCTGGCTACGACCTTTGCGGCGGGTGTTCCCTTGGTCGAAGCACTGG
ATTCCGTGGCAGGGGCTACAGGTAATGTCGTGTTCAGAAATGCAGTGATGAAAATCAAGCAAGATGTGTCCACGGGGATG
CAGCTGAATTTCTCAATGCGCTCCACAGGGGTATTTCCAAGCCTTGCTATCCAGATGACCGCCATCGGTGAAGAGTCTGG
TGCCCTGGATAGCATGCTCGATAAGGTCGCGACCTATTACGAAGATGAAGTCGACAACATGGTCGACAGCCTGACCAGCT
TGATGGAGCCGATGATCATGGCCCTGCTCGGCGTTATCGTCGGAGGGCTGGTCATTGCCATGTATCTTCCGATTTTCCAG
TTGGGAGGTGCGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baumannii D1279779

60.891

99.753

0.607
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Legionella pneumophila strain ERS1305867

52.525

97.778

0.514
  pilG Neisseria gonorrhoeae MS11

42.079

99.753

0.42
  pilG Neisseria meningitidis 44/76-A

42.079

99.753

0.42
  pilC Vibrio campbellii strain DS40M4

40.786

100

0.41
  pilC Vibrio cholerae strain A1552

40.399

99.012

0.4
  pilC Thermus thermophilus HB27

38.272

100

0.383

Multiple sequence alignment