Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EXN22_RS02895 Genome accession   NZ_CP035952
Coordinates   620295..621323 (+) Length   342 a.a.
NCBI ID   WP_130262456.1    Uniprot ID   A0A411MD88
Organism   Pseudomonas tructae strain SNU WT1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 615295..626323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXN22_RS02865 (EXN22_02865) - 615732..616166 (-) 435 WP_130262446.1 DUF4426 domain-containing protein -
  EXN22_RS02870 (EXN22_02870) metW 616191..616811 (-) 621 WP_130262448.1 methionine biosynthesis protein MetW -
  EXN22_RS02875 (EXN22_02875) - 616819..617958 (-) 1140 WP_130262450.1 homoserine O-acetyltransferase -
  EXN22_RS02880 (EXN22_02880) - 618084..618674 (-) 591 WP_045193962.1 YggT family protein -
  EXN22_RS02885 (EXN22_02885) proC 618685..619503 (-) 819 WP_130262452.1 pyrroline-5-carboxylate reductase -
  EXN22_RS02890 (EXN22_02890) - 619542..620228 (-) 687 WP_130262454.1 YggS family pyridoxal phosphate-dependent enzyme -
  EXN22_RS02895 (EXN22_02895) pilT 620295..621323 (+) 1029 WP_130262456.1 type IV pilus twitching motility protein PilT Machinery gene
  EXN22_RS02900 (EXN22_02900) - 621320..622033 (-) 714 WP_407691954.1 C40 family peptidase -
  EXN22_RS02905 (EXN22_02905) - 622203..622622 (+) 420 WP_130262460.1 TM2 domain-containing protein -
  EXN22_RS02910 (EXN22_02910) - 622671..623360 (-) 690 WP_130262462.1 SPOR domain-containing protein -
  EXN22_RS02915 (EXN22_02915) argS 623362..625098 (-) 1737 WP_130262464.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 36671.25 Da        Isoelectric Point: 7.3203

>NTDB_id=345229 EXN22_RS02895 WP_130262456.1 620295..621323(+) (pilT) [Pseudomonas tructae strain SNU WT1]
MDVTQLLALAVQAGASDLHLAAGEVPALRLEGRLRPMTLPRLTPGQMNELLAQLMSAAQQKDFETHLDIDFAFAVPALAR
FRVNAFQQHQGAAAVLRVIPAQVSSLAELGLGDVFRQIAELPGGLVLVTGPTGSGKSTTLAALVDHLNRERSLHIVTLED
PIEFIHRSQRSRISQREIGRHSHGFAQALRAALRQDPNVIMLGELRDPQTIGLALTAAETGHLVLATVHTGSAAKTIDRL
VDVFAAQEKQWVRAMLAEALQAVVSQVLIERVGGGRVAAHEVLLATPAVRNLIREGKVAQLQSVIQTGGAQGMQTLEGCL
QDLLRRGLISPDSAPERAPGQF

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=345229 EXN22_RS02895 WP_130262456.1 620295..621323(+) (pilT) [Pseudomonas tructae strain SNU WT1]
ATGGATGTGACGCAACTGCTGGCGCTGGCAGTGCAGGCTGGCGCCAGCGACCTGCACCTGGCAGCGGGCGAGGTCCCGGC
GCTACGGCTTGAAGGGCGTTTGCGGCCGATGACGCTGCCACGGCTGACACCCGGGCAGATGAATGAATTGCTCGCTCAGC
TCATGTCCGCCGCGCAGCAAAAGGATTTTGAAACACATCTGGATATCGACTTTGCCTTTGCCGTGCCAGCATTGGCGCGC
TTTCGGGTCAATGCATTCCAGCAACACCAGGGCGCCGCTGCAGTGCTGCGGGTGATTCCGGCACAGGTCAGCAGCCTCGC
CGAGCTCGGTCTCGGGGATGTGTTTCGGCAGATTGCCGAACTGCCGGGCGGGCTGGTGCTGGTCACCGGGCCCACCGGTT
CGGGTAAATCAACCACCCTGGCAGCATTGGTCGATCACCTGAACCGTGAGCGGTCGCTGCATATCGTTACCCTCGAGGAC
CCCATCGAGTTTATCCACCGCTCGCAGCGCAGCCGGATCAGCCAGCGCGAAATCGGCCGACACAGCCATGGCTTTGCCCA
GGCCTTGCGCGCCGCGTTGCGCCAGGATCCGAATGTGATCATGCTCGGGGAGCTGCGTGACCCGCAGACCATCGGCCTGG
CCCTGACTGCGGCGGAAACCGGGCACCTGGTGCTGGCCACGGTGCACACCGGCTCGGCAGCAAAAACCATCGACCGCTTG
GTGGATGTGTTTGCTGCCCAAGAGAAGCAGTGGGTGCGTGCCATGCTCGCCGAGGCGTTGCAGGCGGTGGTGTCGCAGGT
GCTGATCGAACGCGTTGGCGGTGGCCGGGTGGCGGCCCATGAGGTTCTGCTGGCAACACCTGCCGTGCGCAACCTGATCC
GCGAGGGCAAGGTGGCGCAGTTGCAATCGGTGATTCAGACAGGTGGGGCGCAGGGCATGCAAACCCTGGAAGGTTGCCTG
CAGGATCTGCTGCGCCGAGGGCTGATCAGCCCTGACAGCGCCCCGGAGCGGGCGCCGGGGCAGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411MD88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

59.302

100

0.596
  pilT Pseudomonas aeruginosa PAK

58.43

100

0.588
  pilT Acinetobacter nosocomialis M2

57.784

97.661

0.564
  pilT Acinetobacter baumannii D1279779

57.784

97.661

0.564
  pilT Acinetobacter baumannii strain A118

57.784

97.661

0.564
  pilT Acinetobacter baylyi ADP1

58.182

96.491

0.561
  pilT Neisseria meningitidis 8013

55.162

99.123

0.547
  pilT Neisseria gonorrhoeae MS11

54.867

99.123

0.544
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

96.491

0.523
  pilT Vibrio cholerae strain A1552

54.242

96.491

0.523
  pilT Legionella pneumophila strain Lp02

52.663

98.83

0.52
  pilT Legionella pneumophila strain ERS1305867

52.663

98.83

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.512
  pilU Pseudomonas stutzeri DSM 10701

38.507

97.953

0.377
  pilU Acinetobacter baylyi ADP1

38.138

97.368

0.371

Multiple sequence alignment