Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DD1591_RS03785 Genome accession   NC_012912
Coordinates   874626..875648 (+) Length   340 a.a.
NCBI ID   WP_012768520.1    Uniprot ID   C6CKZ1
Organism   Dickeya chrysanthemi Ech1591     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 869626..880648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DD1591_RS03755 (Dd1591_0755) hemW 870363..871502 (-) 1140 WP_012768514.1 radical SAM family heme chaperone HemW -
  DD1591_RS03760 (Dd1591_0756) - 871495..872088 (-) 594 WP_012768515.1 XTP/dITP diphosphatase -
  DD1591_RS03765 (Dd1591_0757) yggU 872135..872425 (-) 291 WP_027711004.1 DUF167 family protein YggU -
  DD1591_RS03770 (Dd1591_0758) - 872422..872976 (-) 555 WP_012768517.1 YggT family protein -
  DD1591_RS03775 (Dd1591_0759) proC 873024..873845 (-) 822 WP_012768518.1 pyrroline-5-carboxylate reductase -
  DD1591_RS03780 (Dd1591_0760) - 873879..874604 (-) 726 WP_012768519.1 YggS family pyridoxal phosphate-dependent enzyme -
  DD1591_RS03785 (Dd1591_0761) pilT 874626..875648 (+) 1023 WP_012768520.1 type IV pilus twitching motility protein PilT Machinery gene
  DD1591_RS03790 (Dd1591_0762) - 875671..877743 (-) 2073 WP_238550821.1 bifunctional diguanylate cyclase/phosphodiesterase -
  DD1591_RS03795 (Dd1591_0763) codA 878125..879378 (-) 1254 WP_012768522.1 cytosine deaminase -
  DD1591_RS03800 (Dd1591_0764) - 879392..880327 (-) 936 WP_012768523.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37075.29 Da        Isoelectric Point: 6.2765

>NTDB_id=34447 DD1591_RS03785 WP_012768520.1 874626..875648(+) (pilT) [Dickeya chrysanthemi Ech1591]
MDVSEWVNASVKQNVSDLHLCSGHPPVWRIDGQLVTDVTRPPLQHAELEQWCQHWLEPACEQQLQQYGQTDCALTLPGGQ
RLRANLFMQRHGLSAALRLIPSQQPDLPALGAPPVVAELVDRDDGLILVTGATGSGKSTTLAAMIAALNQRRARHIITLE
DPIEFLHTSRCCLIQQREIGRHSEGFAGALRAALREDPDVLLLGELRDGETIRLALTAAETGHLVLATLHTRRAVQAVDR
LVDVFPAEEKAFVRAQLAASLQAVVAQKLLPGAQRGRVALFEILLATPAVSNLIREGKTHQLPSLLQTGSQVGMQTFEQS
YRQRCREGALPDDGADDAAS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=34447 DD1591_RS03785 WP_012768520.1 874626..875648(+) (pilT) [Dickeya chrysanthemi Ech1591]
ATGGATGTGAGTGAATGGGTGAACGCTAGTGTAAAACAGAACGTATCCGATCTGCACCTGTGCAGCGGTCATCCGCCGGT
GTGGCGTATCGACGGGCAATTGGTGACGGATGTTACCCGGCCGCCGTTGCAGCACGCCGAGCTGGAACAGTGGTGTCAGC
ATTGGCTGGAGCCGGCCTGCGAACAACAGTTGCAGCAATACGGACAAACCGATTGTGCGTTAACCCTGCCGGGAGGACAA
CGGCTACGCGCCAATCTGTTTATGCAGCGTCACGGCTTGTCGGCCGCGCTGCGCTTGATTCCGTCGCAGCAGCCTGACTT
GCCGGCGCTGGGCGCGCCGCCGGTGGTAGCGGAGTTGGTCGATCGCGATGATGGCCTGATTCTGGTGACGGGGGCGACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGATCGCCGCGCTGAATCAGCGCCGGGCGCGACACATTATTACGCTGGAA
GACCCGATAGAGTTTCTGCATACCAGCCGGTGTTGTCTGATCCAGCAGCGCGAAATCGGCCGTCATAGCGAGGGGTTCGC
CGGCGCGTTGCGGGCCGCGTTACGTGAAGACCCGGATGTCCTGCTGTTGGGCGAGTTGCGCGACGGCGAAACCATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGGCGACGCTGCATACCCGGCGGGCCGTTCAAGCGGTAGACCGG
CTGGTGGATGTGTTCCCCGCCGAGGAGAAAGCCTTTGTGCGGGCGCAACTGGCCGCAAGTTTGCAGGCGGTGGTGGCGCA
GAAATTACTGCCGGGCGCTCAGCGCGGACGCGTGGCGTTGTTCGAGATACTGCTTGCTACGCCGGCGGTCAGCAACCTGA
TCCGCGAAGGCAAAACCCACCAGTTGCCCAGCCTGTTGCAAACCGGCAGCCAGGTGGGGATGCAAACCTTTGAGCAGAGT
TATCGGCAGCGCTGCCGCGAAGGGGCGCTGCCGGATGATGGCGCTGACGACGCCGCATCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6CKZ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

48.673

99.706

0.485
  pilT Vibrio cholerae strain A1552

50

97.059

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

97.059

0.485
  pilT Pseudomonas aeruginosa PAK

48.235

100

0.482
  pilT Acinetobacter baumannii D1279779

47.941

100

0.479
  pilT Acinetobacter nosocomialis M2

47.941

100

0.479
  pilT Acinetobacter baumannii strain A118

47.941

100

0.479
  pilT Acinetobacter baylyi ADP1

49.091

97.059

0.476
  pilT Legionella pneumophila strain Lp02

47.493

99.706

0.474
  pilT Legionella pneumophila strain ERS1305867

47.493

99.706

0.474
  pilT Neisseria meningitidis 8013

48.036

97.353

0.468
  pilT Neisseria gonorrhoeae MS11

47.321

98.824

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.887

93.824

0.412
  pilU Pseudomonas stutzeri DSM 10701

37.758

99.706

0.376
  pilU Vibrio cholerae strain A1552

38.554

97.647

0.376

Multiple sequence alignment