Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EWI30_RS04055 Genome accession   NZ_CP035738
Coordinates   839838..840836 (+) Length   332 a.a.
NCBI ID   WP_129995021.1    Uniprot ID   -
Organism   Enterobacter cloacae strain CZ-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 834838..845836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWI30_RS04015 (EWI30_04015) - 835174..835500 (+) 327 WP_014885198.1 YggL family protein -
  EWI30_RS04020 (EWI30_04020) - 835557..836273 (+) 717 WP_129995018.1 DUF2884 domain-containing protein -
  EWI30_RS04025 (EWI30_04025) - 836305..836463 (-) 159 Protein_763 DUF559 domain-containing protein -
  EWI30_RS04030 (EWI30_04030) hemW 836506..837648 (-) 1143 WP_129995019.1 radical SAM family heme chaperone HemW -
  EWI30_RS04035 (EWI30_04035) - 837641..838234 (-) 594 WP_114979897.1 XTP/dITP diphosphatase -
  EWI30_RS04040 (EWI30_04040) yggU 838238..838534 (-) 297 WP_014885193.1 DUF167 family protein YggU -
  EWI30_RS04045 (EWI30_04045) - 838531..839097 (-) 567 WP_028018384.1 YggT family protein -
  EWI30_RS04050 (EWI30_04050) - 839119..839820 (-) 702 WP_129995020.1 YggS family pyridoxal phosphate-dependent enzyme -
  EWI30_RS04055 (EWI30_04055) pilT 839838..840836 (+) 999 WP_129995021.1 type IV pilus twitching motility protein PilT Machinery gene
  EWI30_RS04060 (EWI30_04060) ruvX 840830..841246 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  EWI30_RS04065 (EWI30_04065) - 841246..841806 (-) 561 WP_028018381.1 YqgE/AlgH family protein -
  EWI30_RS04070 (EWI30_04070) gshB 841894..842841 (-) 948 WP_129995022.1 glutathione synthase -
  EWI30_RS04075 (EWI30_04075) rsmE 842861..843592 (-) 732 WP_114979903.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EWI30_RS04080 (EWI30_04080) endA 843645..844352 (-) 708 WP_114979904.1 deoxyribonuclease I -
  EWI30_RS04085 (EWI30_04085) - 844447..844944 (-) 498 WP_114979905.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36218.40 Da        Isoelectric Point: 6.4973

>NTDB_id=344151 EWI30_RS04055 WP_129995021.1 839838..840836(+) (pilT) [Enterobacter cloacae strain CZ-1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPVVEALLKGWLNDEQQGAWWANGQVDFAVTIAGGQRLRG
SAFKQMKGVSVTLRLLPRSCPQLSSLGVPRAVPELLTNDNGLILVTGATGSGKSTTLAAMVDFLNRNTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDIILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQVGMQNFDQSLAER
RAQGRLQAPCAQ

Nucleotide


Download         Length: 999 bp        

>NTDB_id=344151 EWI30_RS04055 WP_129995021.1 839838..840836(+) (pilT) [Enterobacter cloacae strain CZ-1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCTCG
CTGGCGTAGATTAGGCCGTCTTGAACCGGCACCGTTTCCGCCTCCCGTTGTCGAGGCATTGTTAAAAGGGTGGCTGAATG
ATGAGCAGCAGGGAGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTAACCATTGCAGGAGGCCAGCGTTTGCGCGGC
AGTGCCTTTAAACAGATGAAAGGAGTCTCTGTGACGCTACGGCTGTTGCCGCGAAGCTGTCCGCAACTCTCTTCGCTGGG
GGTACCACGGGCTGTGCCGGAACTGCTAACGAACGACAACGGATTGATTCTGGTCACGGGCGCGACCGGCAGCGGCAAAT
CCACCACGCTGGCGGCAATGGTCGATTTTCTCAACCGTAATACTGACGGCCATATCCTGACGCTCGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGCTGCCTGATACAGCAGCGAGAGATTGGCCAACACAGTCCGTCATTCGCCGAGGCGCTACG
CAGCGCGTTACGCGAGGATCCTGACATTATTCTGCTGGGGGAACTGCGCGACAGCGAAACGATCCGTCTGGCGCTGACGG
CGGCGGAAACCGGGCATCTGGTGCTGGCAACCTTGCATACGCGCGGTGCATCTCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCTCAGGAGAAAGATCCGGTGCGTAATCAACTGGCCGGCAGCCTGCGGGCGGTACTGGCGCAAAAGCTACTCCC
CGATCTACAGGGCGGGCGCGTGGCGCTGTATGAACTGCTGGTGAACACAGCGGCTGCAGCGAATCTCATTCGTGAAGGAA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAACAGGTGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAAAGG
CGGGCACAGGGGCGACTTCAGGCTCCCTGTGCTCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.657

100

0.491
  pilT Vibrio cholerae strain A1552

48.657

100

0.491
  pilT Legionella pneumophila strain Lp02

49.689

96.988

0.482
  pilT Legionella pneumophila strain ERS1305867

49.689

96.988

0.482
  pilT Pseudomonas stutzeri DSM 10701

47.463

100

0.479
  pilT Acinetobacter baumannii D1279779

47.164

100

0.476
  pilT Acinetobacter baumannii strain A118

47.164

100

0.476
  pilT Acinetobacter baylyi ADP1

46.866

100

0.473
  pilT Neisseria gonorrhoeae MS11

46.866

100

0.473
  pilT Neisseria meningitidis 8013

47.147

100

0.473
  pilT Pseudomonas aeruginosa PAK

47.706

98.494

0.47
  pilT Acinetobacter nosocomialis M2

48.438

96.386

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

96.988

0.416
  pilU Pseudomonas stutzeri DSM 10701

39.394

99.398

0.392
  pilU Vibrio cholerae strain A1552

39.13

96.988

0.38

Multiple sequence alignment