Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EAT1B_RS13620 Genome accession   NC_012673
Coordinates   2632170..2633207 (+) Length   345 a.a.
NCBI ID   WP_015881136.1    Uniprot ID   -
Organism   Exiguobacterium sp. AT1b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2627170..2638207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAT1B_RS13595 (EAT1b_2656) - 2627874..2628437 (-) 564 WP_237708244.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EAT1B_RS13600 (EAT1b_2657) - 2628385..2628840 (-) 456 WP_015881132.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EAT1B_RS13605 (EAT1b_2658) - 2628998..2630218 (+) 1221 WP_015881133.1 G5 domain-containing protein -
  EAT1B_RS13610 (EAT1b_2659) - 2630215..2630505 (+) 291 WP_015881134.1 hypothetical protein -
  EAT1B_RS13615 (EAT1b_2660) - 2630507..2632177 (+) 1671 WP_015881135.1 GspE/PulE family protein -
  EAT1B_RS13620 (EAT1b_2661) pilT 2632170..2633207 (+) 1038 WP_015881136.1 type IV pilus twitching motility protein PilT Machinery gene
  EAT1B_RS13625 (EAT1b_2662) - 2633214..2634431 (+) 1218 WP_015881137.1 type II secretion system F family protein -
  EAT1B_RS13630 (EAT1b_2663) - 2634572..2635051 (+) 480 WP_015881138.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EAT1B_RS13635 (EAT1b_2664) - 2635139..2635891 (+) 753 WP_015881139.1 A24 family peptidase -
  EAT1B_RS13640 (EAT1b_2665) pilM 2635904..2637475 (+) 1572 WP_015881140.1 pilus assembly protein PilM -
  EAT1B_RS13645 (EAT1b_2666) - 2637472..2638179 (+) 708 WP_015881141.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38431.24 Da        Isoelectric Point: 6.4348

>NTDB_id=34112 EAT1B_RS13620 WP_015881136.1 2632170..2633207(+) (pilT) [Exiguobacterium sp. AT1b]
MINQSIETILTNAVEKRASDIHLTAGSPPIIRVDGSLITMGEEKVLPHHLEGYLKEIMTDDMRERLEHDRDLDFSFGVPR
VSRFRVNAYYQRGTIAIAFRSISGEIPTLNELNLPTVLKRLIEKPHGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIIT
LEDPIEYLHSHDQSIVDQREIGSDVMKFVSGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
SRIIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRIDVPKGRVAAMEIMLGNPAVANLIRNEKEFQLPTVIQTSRQHGMQS
MDAAIEQLVRQGIINPNAVPTEEMR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=34112 EAT1B_RS13620 WP_015881136.1 2632170..2633207(+) (pilT) [Exiguobacterium sp. AT1b]
ATGATTAATCAATCGATTGAAACCATTTTGACGAATGCGGTCGAGAAACGTGCCTCGGATATTCACCTGACAGCCGGTTC
CCCACCGATTATTCGAGTAGATGGGAGTCTCATTACGATGGGCGAGGAGAAGGTATTGCCGCACCATCTTGAAGGATATT
TAAAAGAAATTATGACCGATGATATGCGCGAACGTCTCGAGCATGATCGGGATCTCGATTTTTCGTTCGGTGTCCCACGT
GTTTCCCGTTTTCGGGTCAACGCCTACTATCAACGAGGCACGATTGCCATCGCATTCCGGTCCATTTCCGGGGAAATCCC
GACATTGAACGAGCTCAATCTGCCGACGGTGCTGAAGCGTTTGATTGAGAAACCACATGGTCTCATCTTGGTCACGGGTC
CGACGGGTTCAGGGAAATCGACGACGCTCGCGGCGATGATTCATGAGATTAACCGCACGCAGCGAAAGCGGATCATCACG
CTAGAAGATCCGATCGAGTATTTGCATTCCCATGATCAAAGTATCGTCGACCAACGGGAAATCGGCTCTGACGTGATGAA
ATTTGTGAGTGGTCTACGGGCTGCCTTACGTCAAGACCCGGACGTCATTTTGCTTGGGGAGATGCGGGACTTGGAGACCA
TCTCGACGGCGATGACCGCCGCAGAGACAGGGCACCTCGTCATGGCGACGCTCCATACATCGAGTGCGATGTCGACGGTG
AGTCGAATCATCGACGCATTCCCAGCAGAGCAACAAGACCAAATCCGGACACAACTCGCCAACGTCCTGCTTGGCGTCGT
GAGTCAACGGTTATTTCCACGGATTGATGTGCCGAAAGGCCGCGTCGCCGCCATGGAAATCATGTTGGGGAATCCGGCAG
TTGCCAACTTGATTCGAAATGAAAAAGAGTTCCAGTTGCCGACCGTCATCCAGACGAGTCGCCAACACGGGATGCAGTCG
ATGGATGCCGCCATCGAACAACTCGTTCGACAAGGGATTATCAACCCGAACGCCGTCCCGACAGAAGAGATGAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.76

95.362

0.484
  pilT Acinetobacter baylyi ADP1

48.788

95.652

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.181

97.681

0.461
  pilT Vibrio cholerae strain A1552

47.181

97.681

0.461
  pilT Pseudomonas aeruginosa PAK

47.879

95.652

0.458
  pilT Acinetobacter baumannii D1279779

47.024

97.391

0.458
  pilT Acinetobacter nosocomialis M2

47.024

97.391

0.458
  pilT Acinetobacter baumannii strain A118

47.024

97.391

0.458
  pilT Neisseria meningitidis 8013

47.289

96.232

0.455
  pilT Neisseria gonorrhoeae MS11

46.547

96.522

0.449
  pilT Legionella pneumophila strain Lp02

46.667

95.652

0.446
  pilT Legionella pneumophila strain ERS1305867

46.667

95.652

0.446
  pilT Pseudomonas stutzeri DSM 10701

45.946

96.522

0.443
  pilU Vibrio cholerae strain A1552

41.463

95.072

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.426

95.362

0.386

Multiple sequence alignment