Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DEIDE_RS11245 Genome accession   NC_012526
Coordinates   2362981..2364231 (-) Length   416 a.a.
NCBI ID   WP_012694082.1    Uniprot ID   -
Organism   Deinococcus deserti VCD115     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2357981..2369231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DEIDE_RS11225 (Deide_19564) - 2358115..2358309 (-) 195 WP_041227243.1 hypothetical protein -
  DEIDE_RS11230 (Deide_19570) clpP 2358457..2359068 (+) 612 WP_242402982.1 ATP-dependent Clp protease proteolytic subunit -
  DEIDE_RS11235 (Deide_19580) clpX 2359065..2360270 (+) 1206 WP_012694080.1 ATP-dependent Clp protease ATP-binding subunit ClpX -
  DEIDE_RS11240 (Deide_19590) lon 2360404..2362830 (+) 2427 WP_012694081.1 endopeptidase La -
  DEIDE_RS11245 (Deide_19600) pilT 2362981..2364231 (-) 1251 WP_012694082.1 type IV pilus twitching motility protein PilT Machinery gene
  DEIDE_RS11250 (Deide_19610) pilB 2364271..2366955 (-) 2685 WP_012694083.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  DEIDE_RS11255 (Deide_19620) - 2367030..2367530 (-) 501 WP_012694084.1 YqeG family HAD IIIA-type phosphatase -
  DEIDE_RS11260 (Deide_19630) pgeF 2367527..2368315 (-) 789 WP_012694085.1 peptidoglycan editing factor PgeF -
  DEIDE_RS11265 (Deide_19640) - 2368433..2369215 (+) 783 WP_012694086.1 enoyl-ACP reductase FabI -

Sequence

Protein


Download         Length: 416 a.a.        Molecular weight: 44830.34 Da        Isoelectric Point: 7.1481

>NTDB_id=33737 DEIDE_RS11245 WP_012694082.1 2362981..2364231(-) (pilT) [Deinococcus deserti VCD115]
MTHASVDITDILRFAAEKGASDVILTVGLPPQFKLHGVYDSQGFEVLPPTETRKLMYSMMNEKQQRIFEEKRELDFSFAL
GEKARFRVNAFMQRGNVGGVMRLIPTKIKSAQEMGLPQQVIDIANSPRGLVLVTGPTGSGKSTTLAAMIDYINATKKMHI
MTIEDPIEFMHTHKQSIINQREVGSDTMDFEAALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRLDGAGRILAYELLIANPAVRALIREGKTYQITSVMQTGAREGMV
TMDAFLANLYRRRMISFDVGSERAVDAKEFARMANDPNAGTGAAASLGVPGGTGPGAFAPAAPAATSGAAAGRGAETGRG
SAPATPAGSSNPFGRR

Nucleotide


Download         Length: 1251 bp        

>NTDB_id=33737 DEIDE_RS11245 WP_012694082.1 2362981..2364231(-) (pilT) [Deinococcus deserti VCD115]
ATGACACATGCGTCTGTAGACATCACCGATATCCTGCGTTTTGCCGCTGAAAAGGGCGCGTCCGACGTGATCCTGACCGT
CGGCCTGCCACCACAGTTCAAGCTGCACGGGGTGTACGATTCTCAAGGCTTTGAGGTATTACCCCCCACCGAGACCCGCA
AGCTGATGTACTCGATGATGAATGAGAAGCAGCAGCGAATCTTCGAGGAGAAGCGCGAGCTGGACTTTTCGTTCGCCCTG
GGCGAGAAGGCGCGCTTTCGTGTGAACGCCTTTATGCAGCGCGGCAATGTGGGCGGTGTAATGCGTCTGATTCCCACCAA
GATCAAGAGTGCCCAGGAAATGGGACTGCCCCAGCAGGTGATCGACATCGCCAACTCGCCGCGTGGTCTGGTGCTGGTCA
CCGGGCCCACGGGTTCGGGCAAAAGCACCACGCTCGCGGCTATGATCGACTACATCAACGCCACCAAGAAAATGCACATC
ATGACCATCGAGGATCCCATCGAGTTCATGCACACGCACAAGCAGAGCATCATCAACCAGCGTGAAGTAGGGAGCGACAC
CATGGACTTCGAGGCAGCGCTGCGCGCGGTGCTGCGTCAGGCCCCCGATGTGATTCTGGTGGGCGAAATGCGTGACTACG
AGACCATCAAGGCAGCCGTTACCGCCGCCGAAACCGGACACCTGGTGATGGGCACGCTGCACACCAACTCGGCACCTGAG
TCCATTGACCGTATCGTGGACGTGTTCCCCGAAGAGCAGCAGGAGCAGATCCGGGTGCAGCTGGCCAACAACCTCGTCGC
AGTCATGACCCAGCAGCTGCTGCCACGCCTGGACGGTGCTGGGCGCATCCTGGCCTATGAACTGCTGATTGCCAACCCGG
CCGTGCGGGCGCTGATCCGCGAAGGCAAGACCTACCAGATCACCAGCGTGATGCAGACCGGGGCGCGTGAGGGCATGGTG
ACCATGGACGCCTTCCTGGCCAACCTGTACCGGCGCCGAATGATCAGCTTCGACGTGGGCAGTGAGCGTGCCGTGGATGC
CAAGGAGTTTGCCCGAATGGCCAACGACCCTAATGCTGGCACCGGAGCGGCTGCCAGCCTCGGTGTCCCTGGCGGCACCG
GACCCGGCGCGTTTGCACCAGCAGCGCCCGCAGCGACTTCGGGAGCGGCTGCTGGCCGGGGCGCAGAGACGGGGAGGGGT
AGTGCACCAGCCACCCCTGCTGGCAGCAGCAACCCATTTGGCCGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

74.766

100

0.769
  pilT Neisseria meningitidis 8013

51.714

84.135

0.435
  pilT Pseudomonas aeruginosa PAK

52.047

82.212

0.428
  pilT Neisseria gonorrhoeae MS11

51.156

83.173

0.425
  pilT Pseudomonas stutzeri DSM 10701

51.304

82.933

0.425
  pilT Acinetobacter nosocomialis M2

52.87

79.567

0.421
  pilT Acinetobacter baumannii strain A118

52.87

79.567

0.421
  pilT Acinetobacter baumannii D1279779

52.87

79.567

0.421
  pilT Acinetobacter baylyi ADP1

52.108

79.808

0.416
  pilT Legionella pneumophila strain Lp02

48.851

83.654

0.409
  pilT Legionella pneumophila strain ERS1305867

48.851

83.654

0.409
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.844

83.173

0.406
  pilT Vibrio cholerae strain A1552

48.844

83.173

0.406

Multiple sequence alignment