Detailed information NTG0033738

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DEIDE_RS11250
• Genome accession: NC_012526
• Coordinates: 2364271..2366955 (-)
• Length: 894 a.a.
• NCBI ID: WP_012694083.1
• Uniprot ID: C1CY06
• Organism: Deinococcus deserti VCD115
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2359271..2371955

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DEIDE_RS11240 (Deide_19590)	lon	2360404..2362830 (+)	2427	WP_012694081.1	endopeptidase La	-
DEIDE_RS11245 (Deide_19600)	pilT	2362981..2364231 (-)	1251	WP_012694082.1	type IV pilus twitching motility protein PilT	Machinery gene
DEIDE_RS11250 (Deide_19610)	pilB	2364271..2366955 (-)	2685	WP_012694083.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
DEIDE_RS11255 (Deide_19620)	-	2367030..2367530 (-)	501	WP_012694084.1	YqeG family HAD IIIA-type phosphatase	-
DEIDE_RS11260 (Deide_19630)	pgeF	2367527..2368315 (-)	789	WP_012694085.1	peptidoglycan editing factor PgeF	-
DEIDE_RS11265 (Deide_19640)	-	2368433..2369215 (+)	783	WP_012694086.1	enoyl-ACP reductase FabI	-
DEIDE_RS11270 (Deide_19650)	-	2369310..2369723 (+)	414	WP_012694087.1	isochorismatase family protein	-
DEIDE_RS11275 (Deide_19660)	-	2369782..2370486 (+)	705	WP_012694088.1	IclR family transcriptional regulator	-

Sequence

Protein

• Length: 894 a.a.
• Molecular weight: 97498.30 Da
• Isoelectric Point: 4.9003

>NTDB_id=33738 DEIDE_RS11250 WP_012694083.1 2364271..2366955(-) (pilB) [Deinococcus deserti VCD115]
MALSIGDRRLGAILLEQGYVNDTDLQKVLVRHSEVGGRLADVLIDTGVVGEKRIARALEEALGLPLVNLLVVTPDPEALR
MIRAQTSLTHQAFPFAVEGGTLRVALVDPLSSLAIESLEDDSNLNIEPYQAMRDQILWAIATYYPELNLTAVSPDDLPES
GNSGGGMLGQRLVARGLITDLQLQQALDAQQQTGEPLGITLVAQKAITEKQLYETLADQIGVPFINDTSAFSPTEDVLGS
MLRADALRLNAVPVDETAQGVTIVTSDSRKREDIEALVGRSVQLVLARPADVDALIERFYPQRGRLGEQMVQQGLLSRAQ
LREALNVQSREGKVKPLGEVILELGFASTDEIHSALQKQNSGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNP
PDAGVASLIPEPTARRYGVIPVRFQGESLVVAMKDPRNVFALDDLKLITGRDVIPAVMAEKDITRLIERYYGNRDMADLN
QKLVQESNKREREAKRNDDMDLSAGLDDNAVVRVVDNLIREAALQEASDIHIEPTETSLRVRYRVDGVLREQPELPKGSS
QSILARIKIMGNLDISERRVPQDGRVRFKKGSIDIDLRLSTLPTVYGEKAVMRLLQKASNIPEVENLGFSAYNMQRYLDV
IEKPNGIFLVTGPTGSGKSFTSFSTLKRIARPEKNTTTIEDPVEYEIPGIVQSQVNPVAGLTFARALRAFLRQDPDIIFV
GEIRDGETAKIAVEAALTGHLVLATLHTNDAPGAITRLEEMGVEHFNIGASVVGVLAQRLVRRVCPECKAPTNADPDVLR
RLGITEDDILGAQLMRGAGCNRCGGTGYKGRTGIHELMVIDDPIRRAIGGGKNASEIRDVATKFSGMKTLRQDGIDKALY
GITTLEEVLAVTSS

Nucleotide

• Length: 2685 bp

>NTDB_id=33738 DEIDE_RS11250 WP_012694083.1 2364271..2366955(-) (pilB) [Deinococcus deserti VCD115]
ATGGCTCTTTCAATTGGTGACCGTCGCCTCGGTGCCATTCTGCTCGAACAGGGGTACGTCAACGATACCGACCTGCAAAA
AGTTCTGGTGCGGCATTCCGAGGTTGGTGGCCGTCTCGCGGACGTTCTGATCGACACAGGTGTAGTAGGCGAAAAACGTA
TCGCACGCGCCCTTGAGGAAGCTCTGGGTCTGCCGCTGGTCAACCTGCTGGTCGTCACGCCTGATCCCGAAGCCCTGCGC
ATGATTCGGGCGCAGACGTCCCTGACGCATCAGGCGTTTCCATTTGCTGTGGAGGGCGGCACGTTGCGGGTGGCCCTCGT
GGACCCGCTGTCAAGCCTGGCCATCGAGTCACTGGAAGACGACAGCAACCTCAACATCGAGCCCTACCAGGCCATGCGTG
ACCAGATTCTGTGGGCCATTGCCACCTACTATCCCGAGCTGAACCTGACAGCGGTTTCGCCGGATGATCTGCCCGAGTCA
GGCAACAGCGGTGGAGGCATGCTGGGCCAGCGCCTGGTTGCACGTGGGCTGATCACGGACCTGCAGTTGCAGCAGGCGCT
GGACGCCCAGCAGCAGACCGGCGAGCCACTCGGAATCACCCTGGTGGCCCAGAAGGCCATCACAGAAAAGCAGCTGTATG
AGACCCTGGCTGATCAGATTGGGGTGCCGTTTATCAATGACACCAGTGCCTTCAGTCCGACAGAGGATGTGCTGGGCAGC
ATGCTGCGGGCAGACGCCCTGCGCCTGAATGCCGTGCCGGTGGATGAAACCGCGCAGGGTGTGACGATCGTAACCAGCGA
CTCCCGCAAGCGCGAGGACATCGAGGCCCTGGTGGGCCGCAGTGTTCAACTGGTTCTGGCCCGCCCCGCCGACGTGGACG
CGCTGATCGAGCGTTTCTACCCACAGCGTGGGCGGCTGGGCGAGCAGATGGTCCAGCAGGGCCTGCTCTCGCGTGCGCAG
CTGCGTGAGGCGCTCAACGTGCAGTCGCGCGAGGGCAAGGTCAAGCCGCTGGGGGAGGTGATCCTGGAGCTTGGGTTTGC
CAGTACTGATGAAATTCATAGTGCTCTGCAGAAACAGAATTCCGGGGGCGGGCGTCTGGAAGATACCCTGGTGCAGTCGG
GCAAACTCAGTCCGGAAATGCTGGCGCGCTCACTGGCTGCGCAGCTCGGCTACGAGTTTCTCGACCCGGTCCAGAACCCA
CCGGACGCCGGCGTGGCTTCTCTGATTCCTGAACCTACCGCGCGGCGCTATGGCGTGATCCCGGTGCGTTTCCAGGGCGA
ATCGCTGGTCGTGGCCATGAAGGACCCCCGCAACGTGTTTGCGCTCGACGACCTGAAGCTCATTACGGGTCGTGACGTGA
TTCCAGCGGTCATGGCCGAAAAGGACATCACCCGCCTGATCGAGCGGTACTACGGCAACCGCGACATGGCCGACCTGAAC
CAGAAACTGGTGCAGGAATCCAACAAGCGCGAACGGGAAGCCAAGCGCAATGATGATATGGACCTATCGGCTGGCCTGGA
CGACAACGCCGTGGTCCGCGTCGTGGACAACCTGATCCGTGAAGCGGCGCTACAGGAAGCCAGCGACATTCACATCGAGC
CCACCGAGACCTCGCTGCGTGTACGCTACCGGGTAGATGGAGTGCTGCGTGAGCAGCCGGAACTGCCCAAAGGCAGCTCA
CAGAGCATCCTGGCGCGTATCAAAATCATGGGCAACCTTGACATCTCCGAACGGCGGGTGCCGCAGGACGGCCGCGTGCG
GTTCAAGAAAGGCAGCATCGATATCGATCTGCGTCTGTCCACCCTGCCCACGGTGTACGGGGAGAAAGCTGTGATGCGTC
TGCTGCAGAAGGCCAGCAACATTCCCGAGGTCGAGAACCTGGGGTTTTCCGCGTACAACATGCAGCGCTACCTGGACGTG
ATCGAGAAGCCCAACGGCATCTTCCTGGTGACTGGTCCGACCGGCTCGGGCAAGTCCTTTACCAGCTTCAGCACCCTGAA
ACGCATTGCCCGCCCCGAGAAAAACACCACCACCATCGAGGATCCGGTCGAATACGAGATTCCGGGCATCGTGCAGTCAC
AGGTCAACCCGGTGGCGGGCCTGACCTTCGCCCGCGCGCTGCGCGCCTTCCTGCGTCAGGACCCCGACATCATCTTCGTG
GGGGAAATCCGTGACGGCGAGACGGCCAAGATTGCTGTGGAAGCGGCGCTGACCGGCCACCTGGTCTTGGCCACGCTGCA
CACCAACGACGCTCCTGGCGCGATTACCCGCCTGGAGGAGATGGGGGTGGAGCACTTCAACATCGGTGCGTCTGTGGTCG
GGGTGCTTGCCCAGCGTCTGGTGCGCCGAGTATGCCCGGAATGCAAGGCTCCCACCAACGCTGATCCGGACGTTTTGCGG
CGCCTGGGCATCACCGAGGACGACATTCTGGGCGCGCAACTTATGCGTGGAGCCGGCTGCAACCGCTGTGGTGGGACAGG
CTACAAAGGCCGGACCGGGATTCACGAACTGATGGTGATTGACGACCCAATCCGCCGCGCGATCGGTGGAGGCAAGAACG
CTTCCGAGATCCGCGACGTGGCCACCAAATTCAGCGGCATGAAAACCCTTCGTCAGGACGGGATCGACAAAGCCCTCTAC
GGGATTACCACCTTAGAAGAAGTTCTGGCCGTCACCAGCAGCTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (370-474)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-304)

• Type II/IV secretion system protein (T2SSE) (518-783)

3D structure

• AlphaFold DB: C1CY06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	75.391	100	0.754
pilF	Thermus thermophilus HB27	54.566	100	0.548