Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HRM2_RS02470 Genome accession   NC_012108
Coordinates   568474..569556 (+) Length   360 a.a.
NCBI ID   WP_012662862.1    Uniprot ID   C0QHQ5
Organism   Desulforapulum autotrophicum HRM2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 563474..574556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRM2_RS02440 (HRM2_04930) - 563548..564495 (-) 948 WP_012662856.1 branched-chain amino acid ABC transporter permease -
  HRM2_RS02445 (HRM2_04940) - 564492..565394 (-) 903 WP_012662857.1 branched-chain amino acid ABC transporter permease -
  HRM2_RS02450 (HRM2_04950) - 565482..566618 (-) 1137 WP_012662858.1 branched-chain amino acid ABC transporter substrate-binding protein -
  HRM2_RS02455 (HRM2_04960) - 566643..567416 (-) 774 WP_012662859.1 FadR/GntR family transcriptional regulator -
  HRM2_RS02460 (HRM2_04970) - 567607..567906 (+) 300 WP_012662860.1 YggT family protein -
  HRM2_RS02465 (HRM2_04980) - 567926..568426 (+) 501 WP_012662861.1 DivIVA domain-containing protein -
  HRM2_RS02470 (HRM2_04990) pilT 568474..569556 (+) 1083 WP_012662862.1 type IV pilus twitching motility protein PilT Machinery gene
  HRM2_RS02475 (HRM2_05000) - 569585..570748 (+) 1164 WP_012662863.1 type IV pilus twitching motility protein PilT -
  HRM2_RS02480 (HRM2_05010) - 570745..571368 (+) 624 WP_041272999.1 zinc-ribbon domain-containing protein -
  HRM2_RS02485 (HRM2_05020) - 571372..573234 (+) 1863 WP_012662865.1 bifunctional acetyl-CoA hydrolase/transferase family protein/GNAT family N-acetyltransferase -
  HRM2_RS02490 (HRM2_05030) - 573225..574013 (-) 789 WP_012662866.1 type III pantothenate kinase -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39746.80 Da        Isoelectric Point: 7.5725

>NTDB_id=32995 HRM2_RS02470 WP_012662862.1 568474..569556(+) (pilT) [Desulforapulum autotrophicum HRM2]
MAKIDAFFKLMHDQGASDLHLVAGQPPALRLHGDIERIKYNLLTNDELRGLLYEITPEEKIKVFEETGDVDFGYEIPGLA
RYRANYFMQKYGIGAVFREIPSEILTSEQLGLPSVISKLASLPRGLVLVTGPTGSGKSTTLAAIIDQANRIRRDHIITVE
DPIEFVHKSQGCIVNHREVGLHTKTFSSALRGALREDPDIILVGELRDLETISLAIEAASTGHLVFGTLHTSSAAKTVDR
VIEVFPATQQAQIRSTLADGIRAVVAQTLFKRIDKKGRCAAMEILVATPAVRNLIREAKTHQIPSMIQTGKQYGMQLLDD
AIMQLYKKGWISPDEAYGKANNKSLFRPFLKTAPADFTEA

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=32995 HRM2_RS02470 WP_012662862.1 568474..569556(+) (pilT) [Desulforapulum autotrophicum HRM2]
ATGGCAAAAATCGATGCATTTTTCAAACTCATGCACGACCAGGGCGCTTCAGATTTGCACCTTGTGGCGGGTCAGCCGCC
TGCGCTGAGGCTTCACGGTGATATTGAACGGATCAAGTACAACCTCTTGACCAACGATGAGTTAAGAGGCCTTCTTTACG
AGATCACCCCCGAGGAAAAGATCAAGGTGTTTGAGGAGACTGGAGATGTTGACTTTGGTTATGAAATTCCAGGGCTTGCC
CGTTACAGGGCCAACTATTTCATGCAGAAGTACGGCATCGGAGCCGTGTTCCGGGAAATTCCCTCTGAAATCCTCACATC
AGAGCAGCTCGGGCTGCCTTCGGTCATTTCCAAGCTTGCCTCCCTTCCAAGGGGGCTTGTGCTGGTGACCGGCCCCACTG
GCAGTGGAAAGTCAACCACCCTTGCCGCCATTATTGACCAGGCAAATCGGATCAGAAGGGATCACATCATCACCGTAGAA
GATCCCATCGAGTTTGTCCATAAAAGTCAGGGATGCATCGTCAACCACAGGGAGGTGGGGCTTCATACCAAGACTTTTAG
TTCGGCCTTACGAGGTGCCCTTCGTGAGGATCCCGACATTATCCTGGTTGGTGAGCTAAGGGATCTTGAAACCATTTCCC
TTGCCATTGAAGCCGCCTCCACCGGCCATCTTGTTTTTGGGACCCTCCATACCTCCAGTGCGGCAAAGACCGTTGACAGG
GTTATCGAGGTGTTTCCTGCAACCCAGCAGGCCCAGATTCGATCCACCCTTGCCGACGGCATCCGGGCCGTTGTTGCCCA
GACCCTGTTCAAGCGGATTGACAAAAAGGGCCGTTGTGCCGCCATGGAGATTCTTGTGGCAACCCCTGCCGTTCGAAATC
TCATCCGGGAGGCAAAGACCCACCAGATTCCTTCCATGATCCAGACGGGTAAGCAGTATGGTATGCAGCTCCTTGATGAT
GCCATCATGCAGCTCTACAAAAAAGGATGGATCAGCCCGGACGAGGCCTATGGAAAGGCCAACAACAAATCGCTGTTCAG
GCCCTTCCTCAAGACAGCTCCCGCAGATTTTACCGAAGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C0QHQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

56.757

92.5

0.525
  pilT Pseudomonas aeruginosa PAK

57.798

90.833

0.525
  pilT Legionella pneumophila strain Lp02

56.798

91.944

0.522
  pilT Legionella pneumophila strain ERS1305867

56.798

91.944

0.522
  pilT Acinetobacter nosocomialis M2

54.354

92.5

0.503
  pilT Acinetobacter baumannii D1279779

54.354

92.5

0.503
  pilT Acinetobacter baumannii strain A118

54.354

92.5

0.503
  pilT Acinetobacter baylyi ADP1

54.381

91.944

0.5
  pilT Vibrio cholerae strain A1552

52.41

92.222

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.41

92.222

0.483
  pilT Neisseria meningitidis 8013

51.988

90.833

0.472
  pilT Neisseria gonorrhoeae MS11

51.682

90.833

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.443

97.778

0.464
  pilU Pseudomonas stutzeri DSM 10701

38.968

96.944

0.378
  pilU Vibrio cholerae strain A1552

37.288

98.333

0.367

Multiple sequence alignment