Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DSB70_RS19610 Genome accession   NZ_CP034213
Coordinates   3820386..3821375 (+) Length   329 a.a.
NCBI ID   WP_125060487.1    Uniprot ID   -
Organism   Escherichia albertii strain NCTC 9362     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3815386..3826375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSB70_RS19580 - 3815991..3816998 (+) 1008 WP_125060484.1 DUF1202 family protein -
  DSB70_RS19585 hemW 3817063..3818199 (-) 1137 WP_125060485.1 radical SAM family heme chaperone HemW -
  DSB70_RS19590 - 3818192..3818785 (-) 594 WP_001174776.1 XTP/dITP diphosphatase -
  DSB70_RS19595 yggU 3818793..3819083 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  DSB70_RS19600 yggT 3819080..3819646 (-) 567 WP_125060486.1 osmotic shock tolerance protein YggT -
  DSB70_RS19605 yggS 3819664..3820368 (-) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  DSB70_RS19610 pilT 3820386..3821375 (+) 990 WP_125060487.1 type IV pilus twitching motility protein PilT Machinery gene
  DSB70_RS19615 ruvX 3821547..3821963 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  DSB70_RS19620 - 3821963..3822526 (-) 564 WP_002461296.1 YqgE/AlgH family protein -
  DSB70_RS19625 gshB 3822635..3823585 (-) 951 WP_000593300.1 glutathione synthase -
  DSB70_RS19630 rsmE 3823598..3824329 (-) 732 WP_107192329.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DSB70_RS19635 endA 3824409..3825116 (-) 708 WP_002461298.1 deoxyribonuclease I -
  DSB70_RS19640 - 3825211..3825708 (-) 498 WP_125060488.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 329 a.a.        Molecular weight: 36248.35 Da        Isoelectric Point: 5.5860

>NTDB_id=329871 DSB70_RS19610 WP_125060487.1 3820386..3821375(+) (pilT) [Escherichia albertii strain NCTC 9362]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGATGSGKSTTLAAMVGYLNQHTDAHILTLED
PVEYLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERL
VDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSY
QQRVGEGRL

Nucleotide


Download         Length: 990 bp        

>NTDB_id=329871 DSB70_RS19610 WP_125060487.1 3820386..3821375(+) (pilT) [Escherichia albertii strain NCTC 9362]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGACTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTTATTCTGGTGACGGGGGCGACGGGGGCGACGGGGA
GCGGCAAATCCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGAT
CCTGTGGAATATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATC
GGGATTACGTGCGGCATTGCGGGAAGATCCCGATGTCATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGCCTGG
CGTTGACGGCGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCAGCTCAGGCGGTCGAGCGGCTG
GTGGATTCGTTTCCGGCTCAGGAAAAAGATCCTGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAA
GCTGGAAGTGGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTC
GCGAAGGGAAAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTAT
CAGCAGCGGGTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

100

0.495
  pilT Vibrio cholerae strain A1552

49.394

100

0.495
  pilT Neisseria meningitidis 8013

47.432

100

0.477
  pilT Neisseria gonorrhoeae MS11

47.13

100

0.474
  pilT Acinetobacter baylyi ADP1

46.364

100

0.465
  pilT Acinetobacter baumannii D1279779

46.061

100

0.462
  pilT Acinetobacter baumannii strain A118

46.061

100

0.462
  pilT Acinetobacter nosocomialis M2

46.061

100

0.462
  pilT Pseudomonas stutzeri DSM 10701

46.061

100

0.462
  pilT Pseudomonas aeruginosa PAK

45.758

100

0.459
  pilT Legionella pneumophila strain ERS1305867

44.545

100

0.447
  pilT Legionella pneumophila strain Lp02

44.545

100

0.447
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.433

97.568

0.404
  pilU Vibrio cholerae strain A1552

40.312

97.264

0.392
  pilU Pseudomonas stutzeri DSM 10701

38.209

100

0.389

Multiple sequence alignment