Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AYR02_RS09240 Genome accession   NZ_CP034166
Coordinates   1657434..1658414 (-) Length   326 a.a.
NCBI ID   WP_010336949.1    Uniprot ID   -
Organism   Escherichia albertii strain 2014C-4015     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1652434..1663414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYR02_RS09210 - 1653089..1653586 (+) 498 WP_002461299.1 SprT family zinc-dependent metalloprotease -
  AYR02_RS09215 endA 1653681..1654388 (+) 708 WP_002461298.1 deoxyribonuclease I -
  AYR02_RS09220 rsmE 1654468..1655199 (+) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AYR02_RS09225 gshB 1655212..1656162 (+) 951 WP_000593300.1 glutathione synthase -
  AYR02_RS09230 - 1656271..1656834 (+) 564 WP_002461296.1 YqgE/AlgH family protein -
  AYR02_RS09235 ruvX 1656834..1657250 (+) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  AYR02_RS09240 pilT 1657434..1658414 (-) 981 WP_010336949.1 type IV pilus twitching motility protein PilT Machinery gene
  AYR02_RS09245 yggS 1658432..1659136 (+) 705 WP_059220282.1 pyridoxal phosphate homeostasis protein -
  AYR02_RS09250 yggT 1659154..1659720 (+) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  AYR02_RS09255 yggU 1659717..1660007 (+) 291 WP_059220281.1 DUF167 family protein YggU -
  AYR02_RS09260 - 1660015..1660608 (+) 594 WP_059275583.1 XTP/dITP diphosphatase -
  AYR02_RS09265 hemW 1660601..1661737 (+) 1137 WP_059218090.1 radical SAM family heme chaperone HemW -
  AYR02_RS09270 - 1661802..1662809 (-) 1008 WP_124782482.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36019.12 Da        Isoelectric Point: 5.5860

>NTDB_id=329497 AYR02_RS09240 WP_010336949.1 1657434..1658414(-) (pilT) [Escherichia albertii strain 2014C-4015]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=329497 AYR02_RS09240 WP_010336949.1 1657434..1658414(-) (pilT) [Escherichia albertii strain 2014C-4015]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGATTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393

Multiple sequence alignment