Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CCS91_RS21905 Genome accession   NZ_CP034162
Coordinates   4412786..4413766 (+) Length   326 a.a.
NCBI ID   WP_124866662.1    Uniprot ID   -
Organism   Escherichia albertii strain 06-3542     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4407786..4418766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCS91_RS21875 - 4408391..4409398 (+) 1008 WP_059275585.1 DUF1202 family protein -
  CCS91_RS21880 hemW 4409463..4410599 (-) 1137 WP_059275584.1 radical SAM family heme chaperone HemW -
  CCS91_RS21885 - 4410592..4411185 (-) 594 WP_059275583.1 XTP/dITP diphosphatase -
  CCS91_RS21890 yggU 4411193..4411483 (-) 291 WP_059220281.1 DUF167 family protein YggU -
  CCS91_RS21895 yggT 4411480..4412046 (-) 567 WP_124866661.1 osmotic shock tolerance protein YggT -
  CCS91_RS21900 yggS 4412064..4412768 (-) 705 WP_059220282.1 pyridoxal phosphate homeostasis protein -
  CCS91_RS21905 pilT 4412786..4413766 (+) 981 WP_124866662.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CCS91_RS21910 ruvX 4413932..4414366 (-) 435 WP_059220283.1 Holliday junction resolvase RuvX -
  CCS91_RS21915 - 4414366..4414929 (-) 564 WP_002461296.1 YqgE/AlgH family protein -
  CCS91_RS21920 gshB 4415038..4415988 (-) 951 WP_000593300.1 glutathione synthase -
  CCS91_RS21925 rsmE 4416001..4416732 (-) 732 WP_124866663.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CCS91_RS21930 endA 4416812..4417519 (-) 708 WP_002461298.1 deoxyribonuclease I -
  CCS91_RS21935 - 4417614..4418111 (-) 498 WP_124866664.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36059.23 Da        Isoelectric Point: 5.5860

>NTDB_id=329483 CCS91_RS21905 WP_124866662.1 4412786..4413766(+) (pilT) [Escherichia albertii strain 06-3542]
MNMEEIVALSVKHNVSDLHLCSVWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSEIIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=329483 CCS91_RS21905 WP_124866662.1 4412786..4413766(+) (pilT) [Escherichia albertii strain 06-3542]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGTCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGATTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGATCATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

38.253

100

0.39

Multiple sequence alignment