Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EHN06_RS13940 Genome accession   NZ_CP034142
Coordinates   3067873..3068331 (-) Length   152 a.a.
NCBI ID   WP_206075664.1    Uniprot ID   -
Organism   Marinobacter sp. NP-4(2019)     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3062873..3073331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHN06_RS13920 (EHN06_13920) - 3063443..3064483 (-) 1041 WP_164735614.1 glycosyltransferase family 4 protein -
  EHN06_RS13925 (EHN06_13925) - 3064532..3065386 (-) 855 WP_127333146.1 glycosyltransferase family 2 protein -
  EHN06_RS13930 (EHN06_13930) - 3065413..3066126 (-) 714 WP_127333147.1 hypothetical protein -
  EHN06_RS13935 (EHN06_13935) - 3066123..3067826 (-) 1704 WP_127333148.1 hypothetical protein -
  EHN06_RS13940 (EHN06_13940) pilA 3067873..3068331 (-) 459 WP_206075664.1 pilin Machinery gene
  EHN06_RS13945 (EHN06_13945) pilB 3068725..3070440 (+) 1716 WP_127333149.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EHN06_RS13950 (EHN06_13950) pilC 3070443..3071660 (+) 1218 WP_127333150.1 type II secretion system F family protein Machinery gene
  EHN06_RS13955 (EHN06_13955) pilD 3071791..3072666 (+) 876 WP_127333151.1 prepilin peptidase Machinery gene
  EHN06_RS13960 (EHN06_13960) coaE 3072666..3073265 (+) 600 WP_127333152.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15629.70 Da        Isoelectric Point: 4.1409

>NTDB_id=329332 EHN06_RS13940 WP_206075664.1 3067873..3068331(-) (pilA) [Marinobacter sp. NP-4(2019)]
MKNIQMNQAQKGFTLIELMIVVAIIGILAAVAIPAYQDYTIRAKVTEAMSIASQAKTAVSEYYVSQGSMPADAAAAGLSA
TGTDYETAVVDAMTYGRTSADVGVVTVAVEDLGGDTAAGQNFTLTGTGDSTGVEWVCAAGTLASKYLPSNCR

Nucleotide


Download         Length: 459 bp        

>NTDB_id=329332 EHN06_RS13940 WP_206075664.1 3067873..3068331(-) (pilA) [Marinobacter sp. NP-4(2019)]
ATGAAAAACATTCAGATGAACCAAGCGCAAAAGGGTTTCACGCTGATCGAACTGATGATCGTTGTGGCGATCATTGGTAT
TCTTGCAGCCGTTGCTATTCCGGCGTATCAGGATTACACCATTCGCGCCAAAGTGACAGAGGCAATGTCCATTGCGTCCC
AGGCGAAAACTGCAGTTTCTGAGTACTATGTGTCTCAGGGTTCCATGCCGGCCGACGCCGCCGCAGCTGGTCTCTCGGCC
ACAGGTACTGATTATGAGACTGCAGTCGTAGACGCGATGACATACGGTCGTACTAGCGCGGATGTAGGCGTTGTGACCGT
TGCAGTAGAAGACCTGGGCGGCGACACAGCTGCAGGTCAAAACTTTACTTTGACTGGCACAGGTGACTCAACAGGTGTTG
AGTGGGTTTGTGCGGCCGGCACTCTTGCTTCCAAGTACCTTCCGTCCAATTGTCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)

Pilin (bacterial filament) (Pilin)

(42-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

41.243

100

0.48
  comP Acinetobacter baylyi ADP1

47.059

100

0.474
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.298

100

0.474
  pilA2 Legionella pneumophila strain ERS1305867

48.951

94.079

0.461
  pilA2 Legionella pneumophila str. Paris

48.951

94.079

0.461
  pilE Neisseria gonorrhoeae MS11

40.244

100

0.434
  pilE Neisseria gonorrhoeae strain FA1090

39.024

100

0.421
  pilA/pilA1 Eikenella corrodens VA1

39.241

100

0.408
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.06

99.342

0.408
  pilA Acinetobacter baumannii strain A118

39.456

96.711

0.382
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.364

100

0.368
  pilA Vibrio campbellii strain DS40M4

41.045

88.158

0.362

Multiple sequence alignment