Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EHN06_RS13945 Genome accession   NZ_CP034142
Coordinates   3068725..3070440 (+) Length   571 a.a.
NCBI ID   WP_127333149.1    Uniprot ID   A0A3T0CWN9
Organism   Marinobacter sp. NP-4(2019)     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3063725..3075440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHN06_RS13925 (EHN06_13925) - 3064532..3065386 (-) 855 WP_127333146.1 glycosyltransferase family 2 protein -
  EHN06_RS13930 (EHN06_13930) - 3065413..3066126 (-) 714 WP_127333147.1 hypothetical protein -
  EHN06_RS13935 (EHN06_13935) - 3066123..3067826 (-) 1704 WP_127333148.1 hypothetical protein -
  EHN06_RS13940 (EHN06_13940) pilA 3067873..3068331 (-) 459 WP_206075664.1 pilin Machinery gene
  EHN06_RS13945 (EHN06_13945) pilB 3068725..3070440 (+) 1716 WP_127333149.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EHN06_RS13950 (EHN06_13950) pilC 3070443..3071660 (+) 1218 WP_127333150.1 type II secretion system F family protein Machinery gene
  EHN06_RS13955 (EHN06_13955) pilD 3071791..3072666 (+) 876 WP_127333151.1 prepilin peptidase Machinery gene
  EHN06_RS13960 (EHN06_13960) coaE 3072666..3073265 (+) 600 WP_127333152.1 dephospho-CoA kinase -
  EHN06_RS13965 (EHN06_13965) - 3073258..3074337 (+) 1080 WP_228257310.1 hypothetical protein -
  EHN06_RS13970 (EHN06_13970) tsaA 3074334..3075065 (+) 732 WP_127333153.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62698.88 Da        Isoelectric Point: 5.0916

>NTDB_id=329333 EHN06_RS13945 WP_127333149.1 3068725..3070440(+) (pilB) [Marinobacter sp. NP-4(2019)]
MATNKSNVTLTGLARRFVEDGLLDEATAKDAFIQASQNRIPLITYLVQNKLADSSGLAFSAAMEFGISVLDLSAFLPEML
PEKVVDEKLIRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLHEAIDKYLESHDSTMGDLEDA
DLEGVETEGGEHEDEGAVSPSEVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKVYRVRYRTDGILKEMSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKKLYMDALQ
QPQGMILVTGPTGSGKTVSLYTGLNILNTPELNISTAEDPAEINLEGINQVNVNTKVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCNSCKKPAEVPHDVLIAE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFPNLRQSALKKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=329333 EHN06_RS13945 WP_127333149.1 3068725..3070440(+) (pilB) [Marinobacter sp. NP-4(2019)]
ATGGCTACCAATAAATCCAACGTAACGCTTACCGGCCTTGCACGTCGCTTTGTGGAGGACGGACTGCTTGATGAGGCTAC
CGCGAAGGATGCCTTTATTCAGGCGTCTCAAAATCGCATTCCGCTGATCACTTATCTCGTTCAGAACAAACTGGCGGACA
GCAGCGGGCTGGCTTTCTCCGCCGCCATGGAGTTTGGTATATCCGTTCTGGATCTCAGCGCTTTCCTTCCGGAGATGCTG
CCGGAGAAAGTGGTTGATGAGAAGCTGATCCGGAAACACAACGCACTGCCTCTGTACAAACGTGGCAATCGTCTGTTCAT
TGCTGTCTCTGACCCCACCAATATCCAGGCTCTGGATGAGATCAAGTTCAACACCGGCCTCAGTACGGACGCCATACTGG
TTGATGACGCTAAACTGCATGAGGCCATCGACAAGTACCTGGAATCCCATGACTCTACGATGGGAGACCTTGAAGATGCC
GACTTGGAAGGCGTGGAGACCGAAGGTGGCGAGCACGAAGACGAGGGCGCGGTTAGTCCCAGCGAAGTTGATGATGCGCC
GATCGTAAAATATGTGAATAAAATGCTTCTGGACGCCATTCGGGGAGGCGCGTCGGACATTCACTTTGAGCCTTACGAAA
AAGTGTACCGGGTACGCTACCGAACAGACGGCATTCTGAAAGAGATGTCCCGCCCCTCCATCAAACTGGCTCCCAAGATC
TCGGCGCGGGTGAAGATCATGGCGCAATTGGATATCTCGGAGCGCCGGGTTCCCCAGGATGGCCGGATCAAGATGAAGCT
GTCCAAGACCAAAGCCATCGACTTCCGGGTGAATACGTTGCCGACCCTGTGGGGCGAGAAGATCGTTCTGCGGATCCTTG
ACCCCAGCCAGGCCAAACTGGGTATCGATGTTCTGGGCTATGAAGAAGACCAGAAGAAGCTGTATATGGATGCTCTGCAG
CAACCTCAGGGTATGATTCTGGTGACCGGCCCCACGGGTTCCGGTAAAACTGTTTCCTTGTACACTGGCCTGAACATTCT
GAACACGCCAGAGCTGAATATCTCTACCGCCGAAGACCCAGCCGAGATTAACCTCGAAGGTATTAACCAGGTGAACGTAA
ATACCAAGGTGGGCCTTGGGTTTGCAGAAGCCTTGAGGGCCTTCCTGCGGCAGGACCCCGACGTAATCATGGTGGGCGAG
ATCCGGGATCTGGAAACCGCCAACATTGCCATCAAGGCGGCGCAGACCGGCCACCTGGTACTTTCAACCCTGCACACCAA
CAGCGCAGCGGAAACGCTGACGCGGATGATGAACATGGGGGTACCCGCGTTCAACATTGCTACTTCAGTCAGTCTGATTA
TCGCCCAGCGACTGGGCCGAAGGCTTTGCAACAGCTGTAAGAAGCCTGCGGAGGTCCCGCACGATGTCCTTATCGCCGAA
GGGTTCACACAGGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGAGGATACAA
AGGACGCGTCGGTATTTACGAAGTGGTCAAGATTACCGACGAACTGGCAAACATGATTATGGAAGAAGCCAGCTCCATTA
AAATTGCAAAGCAGGCTCAGGCCGAAGGCTTCCCGAATCTTCGCCAGTCCGCCCTCAAGAAAGTGATTGAGGGGGTGACC
AGCCTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3T0CWN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.993

100

0.571
  pilB Acinetobacter baylyi ADP1

57.371

98.599

0.566
  pilB Legionella pneumophila strain ERS1305867

54.087

100

0.545
  pilB Vibrio campbellii strain DS40M4

51.241

98.774

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

51.978

97.373

0.506
  pilB Vibrio cholerae strain A1552

55.192

91.068

0.503
  pilF Neisseria gonorrhoeae MS11

47.611

98.949

0.471
  pilF Thermus thermophilus HB27

38.087

97.023

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.924

92.119

0.368
  pilB/pilB1 Synechocystis sp. PCC 6803

39.692

90.893

0.361

Multiple sequence alignment