Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EHN06_RS13955 Genome accession   NZ_CP034142
Coordinates   3071791..3072666 (+) Length   291 a.a.
NCBI ID   WP_127333151.1    Uniprot ID   A0A3Q9NIT2
Organism   Marinobacter sp. NP-4(2019)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3066791..3077666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHN06_RS13940 (EHN06_13940) pilA 3067873..3068331 (-) 459 WP_206075664.1 pilin Machinery gene
  EHN06_RS13945 (EHN06_13945) pilB 3068725..3070440 (+) 1716 WP_127333149.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EHN06_RS13950 (EHN06_13950) pilC 3070443..3071660 (+) 1218 WP_127333150.1 type II secretion system F family protein Machinery gene
  EHN06_RS13955 (EHN06_13955) pilD 3071791..3072666 (+) 876 WP_127333151.1 prepilin peptidase Machinery gene
  EHN06_RS13960 (EHN06_13960) coaE 3072666..3073265 (+) 600 WP_127333152.1 dephospho-CoA kinase -
  EHN06_RS13965 (EHN06_13965) - 3073258..3074337 (+) 1080 WP_228257310.1 hypothetical protein -
  EHN06_RS13970 (EHN06_13970) tsaA 3074334..3075065 (+) 732 WP_127333153.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  EHN06_RS13975 (EHN06_13975) - 3075151..3077475 (+) 2325 WP_228257312.1 response regulator -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32246.51 Da        Isoelectric Point: 7.5668

>NTDB_id=329335 EHN06_RS13955 WP_127333151.1 3071791..3072666(+) (pilD) [Marinobacter sp. NP-4(2019)]
MLILDSLLATPWLLYLSVVLFSLCIGSFLNVVILRLPKMMHQDWRCQCEEFLALPEGQRKPETPITLSSPASTCPSCNHR
IRAWENIPVISYLVLGGKCSSCKTRISPRYPMIEALTAIFSVVTIAILGPSVSSLWALLLVWSLVAMTMIDFDTQLLPDS
MTLPLMWLGLVLNYFGVLTDFHSAFWGAVAGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAALGAWLGWQLLPAVILLSSV
VGAVVGISLMVFRKHGREVPIPFGPYLAAAGLLCLWFGAEIQSWWFGFMGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=329335 EHN06_RS13955 WP_127333151.1 3071791..3072666(+) (pilD) [Marinobacter sp. NP-4(2019)]
ATGCTTATTCTTGATTCTCTGCTGGCCACGCCGTGGCTGTTGTATTTGTCGGTGGTGCTTTTTTCGCTGTGTATTGGCAG
TTTCCTGAATGTGGTGATTCTTCGCCTGCCAAAGATGATGCACCAGGATTGGCGCTGCCAGTGCGAGGAGTTTCTGGCGC
TGCCGGAGGGGCAGCGTAAGCCGGAAACGCCCATTACCCTGTCATCTCCCGCCTCTACCTGCCCTTCCTGTAACCACAGG
ATCCGCGCCTGGGAGAATATTCCCGTAATCAGTTACCTGGTGCTGGGGGGCAAGTGCTCTTCCTGCAAGACGCGGATTTC
TCCCCGCTACCCGATGATCGAGGCACTGACGGCGATTTTCTCGGTGGTGACCATTGCGATTCTGGGGCCGTCCGTGTCGT
CGCTTTGGGCACTGCTGCTGGTGTGGTCGCTGGTAGCGATGACGATGATCGATTTTGATACCCAGCTGCTGCCGGATAGC
ATGACCCTGCCGCTGATGTGGCTGGGGCTCGTGCTGAATTATTTTGGTGTGCTGACGGATTTTCACAGTGCCTTCTGGGG
TGCGGTGGCGGGCTATCTGTCGCTGTGGTCGGTGTACTGGCTGTTCAAGCTGGTTACTGGCAAGGAAGGCATGGGGCATG
GGGATTTCAAGTTGTTGGCCGCTTTGGGGGCCTGGCTGGGTTGGCAGTTGCTGCCGGCGGTGATTTTGTTATCGTCCGTG
GTCGGGGCGGTTGTGGGGATATCTTTGATGGTGTTCCGTAAGCATGGGCGTGAGGTGCCGATTCCGTTTGGGCCTTATCT
GGCGGCCGCCGGGTTGCTTTGTTTGTGGTTTGGTGCGGAAATTCAGAGTTGGTGGTTTGGGTTTATGGGGGTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9NIT2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.237

95.533

0.509
  pilD Acinetobacter baumannii D1279779

53.65

94.158

0.505
  pilD Acinetobacter nosocomialis M2

53.285

94.158

0.502
  pilD Vibrio campbellii strain DS40M4

52.158

95.533

0.498
  pilD Neisseria gonorrhoeae MS11

49.618

90.034

0.447

Multiple sequence alignment