Detailed information NTG0328768

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: N032_RS04845
• Genome accession: NZ_CP034078
• Coordinates: 1005852..1006277 (+)
• Length: 141 a.a.
• NCBI ID: WP_003392698.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. pisi str. PP1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1000852..1011277

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N032_RS04820 (N032_04820)	yacG	1001012..1001221 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
N032_RS04825 (N032_04825)	coaE	1001218..1001841 (-)	624	WP_024648208.1	dephospho-CoA kinase	-
N032_RS04830 (N032_04830)	pilD	1001838..1002710 (-)	873	WP_024648207.1	A24 family peptidase	Machinery gene
N032_RS04835 (N032_04835)	pilC	1002712..1003929 (-)	1218	WP_024648206.1	type II secretion system F family protein	Machinery gene
N032_RS04840 (N032_04840)	pilB	1003932..1005626 (-)	1695	WP_024648205.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N032_RS04845 (N032_04845)	pilA2	1005852..1006277 (+)	426	WP_003392698.1	pilin	Machinery gene
N032_RS28525 (N032_04850)	-	1006418..1006589 (+)	172	Protein_896	DUF433 domain-containing protein	-
N032_RS04855 (N032_04855)	-	1006637..1007182 (-)	546	WP_003317774.1	DUF1523 family protein	-
N032_RS04860 (N032_04860)	-	1007302..1008300 (+)	999	WP_024648204.1	ATP-grasp domain-containing protein	-
N032_RS04865 (N032_04865)	-	1008355..1009494 (+)	1140	WP_024648203.1	trypsin-like peptidase domain-containing protein	-
N032_RS04870 (N032_04870)	-	1009561..1010694 (+)	1134	WP_024648202.1	phosphoribosyltransferase domain-containing protein	-

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 14435.43 Da
• Isoelectric Point: 8.4743

>NTDB_id=328768 N032_RS04845 WP_003392698.1 1005852..1006277(+) (pilA2) [Pseudomonas syringae pv. pisi str. PP1]
MRSQKGFTLIELMIVVAIVGILAAVALPAYQDYTVRAQVAELIGLTDGAKVAVTENYQSTGAFPASNQAAGYGGATGRYT
TSVVIGANGLITATADAVNSNPAIRGQTITLAPAVAAAGNNVVTWTCGGTIDQRFRPTSCR

Nucleotide

• Length: 426 bp

>NTDB_id=328768 N032_RS04845 WP_003392698.1 1005852..1006277(+) (pilA2) [Pseudomonas syringae pv. pisi str. PP1]
ATGAGATCACAAAAAGGTTTTACCCTTATTGAATTGATGATTGTTGTTGCAATCGTCGGTATCTTGGCGGCGGTCGCACT
GCCTGCATATCAGGATTATACGGTTCGAGCCCAAGTGGCTGAGCTGATCGGTCTGACTGATGGTGCTAAGGTCGCAGTTA
CTGAAAATTATCAATCAACAGGTGCATTTCCTGCTTCTAACCAAGCTGCTGGCTATGGCGGAGCAACTGGTCGCTATACG
ACTTCTGTAGTGATTGGTGCTAATGGATTGATTACCGCTACAGCTGATGCTGTAAACTCGAACCCTGCAATTCGTGGTCA
GACCATTACGCTGGCTCCGGCTGTGGCAGCCGCAGGTAATAATGTCGTTACATGGACATGCGGCGGTACTATTGACCAGC
GTTTCCGTCCGACGAGCTGCCGATAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-141)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-27)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	54.545	100	0.553
pilA2	Legionella pneumophila strain ERS1305867	54.545	100	0.553
pilE	Neisseria gonorrhoeae MS11	44.654	100	0.504
pilE	Neisseria gonorrhoeae strain FA1090	44.304	100	0.496
pilA/pilA1	Eikenella corrodens VA1	42.949	100	0.475
comP	Acinetobacter baylyi ADP1	40.94	100	0.433
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	36.196	100	0.418
pilA/pilAII	Pseudomonas stutzeri DSM 10701	40.559	100	0.411
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.437	100	0.397
pilA	Haemophilus influenzae 86-028NP	38.889	100	0.397
pilA	Acinetobacter baumannii strain A118	37.333	100	0.397
pilA	Pseudomonas aeruginosa PAK	34.641	100	0.376
pilA	Haemophilus influenzae Rd KW20	37.063	100	0.376