Detailed information NTG0328765

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N032_RS04830
• Genome accession: NZ_CP034078
• Coordinates: 1001838..1002710 (-)
• Length: 290 a.a.
• NCBI ID: WP_024648207.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. pisi str. PP1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 996838..1007710

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N032_RS04795 (N032_04795)	-	997623..998921 (-)	1299	WP_024648210.1	NAD(P)/FAD-dependent oxidoreductase	-
N032_RS04805 (N032_04805)	-	999138..999671 (-)	534	WP_003317783.1	MOSC domain-containing protein	-
N032_RS04810 (N032_04810)	-	999668..1000297 (-)	630	WP_003430735.1	DUF1780 domain-containing protein	-
N032_RS04815 (N032_04815)	-	1000424..1000885 (+)	462	WP_024648209.1	hypothetical protein	-
N032_RS04820 (N032_04820)	yacG	1001012..1001221 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
N032_RS04825 (N032_04825)	coaE	1001218..1001841 (-)	624	WP_024648208.1	dephospho-CoA kinase	-
N032_RS04830 (N032_04830)	pilD	1001838..1002710 (-)	873	WP_024648207.1	A24 family peptidase	Machinery gene
N032_RS04835 (N032_04835)	pilC	1002712..1003929 (-)	1218	WP_024648206.1	type II secretion system F family protein	Machinery gene
N032_RS04840 (N032_04840)	pilB	1003932..1005626 (-)	1695	WP_024648205.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N032_RS04845 (N032_04845)	pilA2	1005852..1006277 (+)	426	WP_003392698.1	pilin	Machinery gene
N032_RS28525 (N032_04850)	-	1006418..1006589 (+)	172	Protein_896	DUF433 domain-containing protein	-
N032_RS04855 (N032_04855)	-	1006637..1007182 (-)	546	WP_003317774.1	DUF1523 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32048.24 Da
• Isoelectric Point: 8.3667

>NTDB_id=328765 N032_RS04830 WP_024648207.1 1001838..1002710(-) (pilD) [Pseudomonas syringae pv. pisi str. PP1]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPQCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAMLSAYVAWHFGYGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=328765 N032_RS04830 WP_024648207.1 1001838..1002710(-) (pilD) [Pseudomonas syringae pv. pisi str. PP1]
ATGCCCCTCCTCGACCTACTGGCCAGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATTCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCCTCCCGATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGAGAACTGCTCGGCC
TGCCCGCCGAACCCGATCAGCCCGTCTTCAACCTGAACCGCCCCCGTTCCAGCTGCCCGCAGTGCGCACACAAAATCCGT
CCATGGGAAAACCTGCCGGTGATCAGCTATCTGCTGCTGCGCGGTAAATGTTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAATGTTGTCGGCCTACGTAGCCTGGCATTTCGGCTATGGCTGGCAGGCGGCGG
CGATGCTGGTACTGAGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCCGATCACCAACTGTTGCCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTTACCTCGCTGAACGATGCGCTTTGGGGCGCGGT
GGCCGGTTATCTGGCCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACAGGTAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTAGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGCTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTTGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.65	97.586	0.514
pilD	Vibrio campbellii strain DS40M4	52.297	97.586	0.51
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.825	94.483	0.49
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472