Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N032_RS04835 Genome accession   NZ_CP034078
Coordinates   1002712..1003929 (-) Length   405 a.a.
NCBI ID   WP_024648206.1    Uniprot ID   A0A3M4Y1D6
Organism   Pseudomonas syringae pv. pisi str. PP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 997712..1008929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N032_RS04805 (N032_04805) - 999138..999671 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  N032_RS04810 (N032_04810) - 999668..1000297 (-) 630 WP_003430735.1 DUF1780 domain-containing protein -
  N032_RS04815 (N032_04815) - 1000424..1000885 (+) 462 WP_024648209.1 hypothetical protein -
  N032_RS04820 (N032_04820) yacG 1001012..1001221 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  N032_RS04825 (N032_04825) coaE 1001218..1001841 (-) 624 WP_024648208.1 dephospho-CoA kinase -
  N032_RS04830 (N032_04830) pilD 1001838..1002710 (-) 873 WP_024648207.1 A24 family peptidase Machinery gene
  N032_RS04835 (N032_04835) pilC 1002712..1003929 (-) 1218 WP_024648206.1 type II secretion system F family protein Machinery gene
  N032_RS04840 (N032_04840) pilB 1003932..1005626 (-) 1695 WP_024648205.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N032_RS04845 (N032_04845) pilA2 1005852..1006277 (+) 426 WP_003392698.1 pilin Machinery gene
  N032_RS28525 (N032_04850) - 1006418..1006589 (+) 172 Protein_896 DUF433 domain-containing protein -
  N032_RS04855 (N032_04855) - 1006637..1007182 (-) 546 WP_003317774.1 DUF1523 family protein -
  N032_RS04860 (N032_04860) - 1007302..1008300 (+) 999 WP_024648204.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44048.96 Da        Isoelectric Point: 10.1744

>NTDB_id=328766 N032_RS04835 WP_024648206.1 1002712..1003929(-) (pilC) [Pseudomonas syringae pv. pisi str. PP1]
MASKAVKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVNSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFAGFGAELPTFTLMVIGLSVFVQEWWLAIIGIFFASIFMFK
KSYKKSQKFRDSLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVIFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAILGVVVGGLVIAMYLPIFK
LGNAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=328766 N032_RS04835 WP_024648206.1 1002712..1003929(-) (pilC) [Pseudomonas syringae pv. pisi str. PP1]
ATGGCCAGCAAGGCAGTCAAAGTCATCGTTTACACTTGGGAAGGTGTTGACAAGAAAGGCACTAAAACCAGCGGCGAACT
GAGCGGCCATAATCTGGCGCTCGTAAAGGCCCAGTTACGCAAGCAAGGCATCAACCCTACCAAGGTGCGTAAAAAGTCTG
CGTCGATATTCGGTAAAGGTAAAAAAATCAAGCCATTGGACATCGCGTTTTTTTCTCGGCAGATGGCAACGATGATGAAG
GCTGGCGTACCATTGCTGCAGTCGTTCGACATAATCAGCGAGGGTGCAGAAAACCCGAACATGCGAACATTGGTAAACTC
TCTGAAGCAGGAAGTTTCAGCAGGTAACAGCTTTGCGACGGCTTTGCGGCAAAAGCCCGAATATTTCGACGACCTCTTTT
GCAATCTTGTGGATGCTGGTGAGCAAGCCGGCGCACTTGAGAGTTTGCTGGACCGAGTTGCGAGCTACAAAGAGAAGACC
GAAAAGCTCAAGGCAAAAATCAAAAAGGCTATGACCTATCCCGCTGCAGTATTAATAGTAGCAGTTATAGTTTCAGGAAT
TCTGCTTATCAAAGTTGTACCACAATTTCAATCAGTGTTCGCTGGATTCGGAGCAGAGCTGCCGACCTTTACTTTGATGG
TTATTGGACTATCCGTTTTCGTACAGGAGTGGTGGCTAGCGATCATCGGGATTTTTTTTGCTTCGATATTTATGTTCAAA
AAATCGTATAAAAAGTCTCAAAAATTTCGCGACAGTCTGGATCGTTTTTTATTAAAAGTGCCGATTATAGGACCGCTAAT
TTTTAAATCTTCAGTAGCCCGCTACGCACGCACACTAGCGACGACGTTTGCAGCTGGCGTCCCGTTGGTAGAGGCGTTGG
ATTCAGTCGCAGGAGCGACCGGCAATGTCATATTCAAGAACGCAGTCAACAAGGTCAAGCAGGACGTCTCGACCGGTATG
CAGCTTAACTTCTCAATGCGCTCCACCGGGGTTTTTCCTAGCTTAGCAATTCAGATGACTGCTATAGGTGAGGAGTCCGG
CGCACTTGACAGTATGCTCGATAAAGTTGCGACCTATTACGAGGATGAGGTAGATAACATGGTAGATAGCCTGACCAGTC
TCATGGAGCCTATGATAATGGCAATCCTAGGCGTGGTTGTCGGAGGTTTAGTAATTGCTATGTACTTACCGATTTTTAAA
TTAGGAAACGCAGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M4Y1D6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.333

100

0.733
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Legionella pneumophila strain ERS1305867

52.381

98.519

0.516
  pilG Neisseria gonorrhoeae MS11

44.724

98.272

0.44
  pilG Neisseria meningitidis 44/76-A

44.472

98.272

0.437
  pilC Vibrio campbellii strain DS40M4

43.257

97.037

0.42
  pilC Vibrio cholerae strain A1552

42.394

99.012

0.42
  pilC Thermus thermophilus HB27

37.656

99.012

0.373

Multiple sequence alignment