Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   EGC82_RS02940 Genome accession   NZ_CP034015
Coordinates   640497..640940 (-) Length   147 a.a.
NCBI ID   WP_124729420.1    Uniprot ID   A0A3G8LQU9
Organism   Shewanella livingstonensis strain LMG 19866     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 635497..645940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGC82_RS02920 (EGC82_02920) coaE 635707..636336 (-) 630 WP_124729416.1 dephospho-CoA kinase -
  EGC82_RS02925 (EGC82_02925) pilD 636333..637259 (-) 927 WP_124729417.1 A24 family peptidase Machinery gene
  EGC82_RS02930 (EGC82_02930) pilC 637292..638554 (-) 1263 WP_124729418.1 type II secretion system F family protein Machinery gene
  EGC82_RS02935 (EGC82_02935) pilB 638687..640393 (-) 1707 WP_124729419.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGC82_RS02940 (EGC82_02940) pilA/pilAI 640497..640940 (-) 444 WP_124729420.1 pilin Machinery gene
  EGC82_RS02945 (EGC82_02945) nadC 641932..642810 (-) 879 WP_124729421.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EGC82_RS02950 (EGC82_02950) ampD 643339..643893 (+) 555 WP_124729422.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EGC82_RS02955 (EGC82_02955) ampE 644031..644882 (+) 852 WP_124729423.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15615.78 Da        Isoelectric Point: 4.2917

>NTDB_id=328007 EGC82_RS02940 WP_124729420.1 640497..640940(-) (pilA/pilAI) [Shewanella livingstonensis strain LMG 19866]
MKSINQIKNAKGFTLIELMIVVAIIGILAAIALPAYQDYTVKSQVNSAYSEVSSIKSQYEAVMNEGKTPSITRADIGFVG
QVTDGGQYCILSLVADNRGLECEITNANADKALGETITLGRDEDGIWTCTTSADLEAKFIPGSCVSA

Nucleotide


Download         Length: 444 bp        

>NTDB_id=328007 EGC82_RS02940 WP_124729420.1 640497..640940(-) (pilA/pilAI) [Shewanella livingstonensis strain LMG 19866]
ATGAAAAGTATCAACCAAATCAAAAACGCTAAGGGTTTCACCCTTATCGAATTAATGATCGTAGTCGCGATTATAGGTAT
TCTTGCAGCAATCGCACTGCCTGCTTATCAGGATTACACAGTTAAGTCACAGGTTAATTCGGCTTACTCTGAAGTGAGTT
CAATTAAATCACAATATGAAGCAGTAATGAATGAAGGTAAAACACCTTCAATAACAAGGGCTGACATTGGCTTTGTTGGT
CAAGTAACAGATGGCGGTCAATACTGCATCTTAAGTTTAGTTGCTGATAATCGTGGTCTTGAGTGCGAAATAACTAATGC
TAATGCAGACAAAGCTTTAGGTGAAACAATTACTTTAGGAAGAGATGAAGACGGTATATGGACTTGTACTACTTCTGCAG
ATTTAGAGGCAAAGTTTATTCCAGGATCTTGCGTATCAGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-33)

Pilin (bacterial filament) (Pilin)

(41-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G8LQU9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

54.815

91.837

0.503
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.89

99.32

0.456
  pilA Acinetobacter baumannii strain A118

45.255

93.197

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

34.091

100

0.408
  pilA Pseudomonas aeruginosa PAK

38.411

100

0.395
  pilE Neisseria gonorrhoeae MS11

37.013

100

0.388
  pilA2 Legionella pneumophila strain ERS1305867

41.481

91.837

0.381
  pilA2 Legionella pneumophila str. Paris

41.481

91.837

0.381
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.5

97.959

0.367
  pilA Vibrio cholerae strain A1552

37.5

97.959

0.367
  pilA Vibrio cholerae C6706

37.5

97.959

0.367
  comP Acinetobacter baylyi ADP1

37.762

97.279

0.367
  pilA Vibrio parahaemolyticus RIMD 2210633

39.259

91.837

0.361

Multiple sequence alignment