Detailed information NTG0328004

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EGC82_RS02925
• Genome accession: NZ_CP034015
• Coordinates: 636333..637259 (-)
• Length: 308 a.a.
• NCBI ID: WP_124729417.1
• Uniprot ID: A0A3G8LQG4
• Organism: Shewanella livingstonensis strain LMG 19866
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 631333..642259

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGC82_RS02895 (EGC82_02895)	-	632533..633504 (+)	972	Protein_551	IS630 family transposase	-
EGC82_RS02900 (EGC82_02900)	-	633572..634105 (-)	534	Protein_552	IS630 family transposase	-
EGC82_RS02905 (EGC82_02905)	mutT	634290..634688 (-)	399	WP_124729413.1	8-oxo-dGTP diphosphatase MutT	-
EGC82_RS02910 (EGC82_02910)	yacG	634725..634937 (-)	213	WP_124729414.1	DNA gyrase inhibitor YacG	-
EGC82_RS02915 (EGC82_02915)	zapD	634980..635714 (-)	735	WP_124729415.1	cell division protein ZapD	-
EGC82_RS02920 (EGC82_02920)	coaE	635707..636336 (-)	630	WP_124729416.1	dephospho-CoA kinase	-
EGC82_RS02925 (EGC82_02925)	pilD	636333..637259 (-)	927	WP_124729417.1	A24 family peptidase	Machinery gene
EGC82_RS02930 (EGC82_02930)	pilC	637292..638554 (-)	1263	WP_124729418.1	type II secretion system F family protein	Machinery gene
EGC82_RS02935 (EGC82_02935)	pilB	638687..640393 (-)	1707	WP_124729419.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EGC82_RS02940 (EGC82_02940)	pilA/pilAI	640497..640940 (-)	444	WP_124729420.1	pilin	Machinery gene

Sequence

Protein

• Length: 308 a.a.
• Molecular weight: 34273.82 Da
• Isoelectric Point: 7.6802

>NTDB_id=328004 EGC82_RS02925 WP_124729417.1 636333..637259(-) (pilD) [Shewanella livingstonensis strain LMG 19866]
MTEFISTFIATMTQYPGVFVAISFIFAVTIGSFLNVVIHRLPVMMKREWQSECNFYLNEYHPDIVEQIGNERLNKPIDAF
PAKYNIVVPGSACPKCKANIKPWHNLPILGWLMLKGKCADCKTSISSRYPIIELLTGVLIAALAWHFGPTWQFVYASILT
FVLVALTGIDLDEMLLPDQLTLPILWLGLIINLKGIYTSPEDAIIGAAAGYLSLWSVFWLFKLITGKEGMGYGDFKLLAV
FGAWLGWQMLPLIILLSSLVGAVVGITMIMAKKLNKGNPIPFGPYIAAAGWIALVWGHDILNWYLSTL

Nucleotide

• Length: 927 bp

>NTDB_id=328004 EGC82_RS02925 WP_124729417.1 636333..637259(-) (pilD) [Shewanella livingstonensis strain LMG 19866]
ATGACTGAATTTATCTCAACTTTTATTGCCACAATGACTCAATACCCTGGGGTCTTTGTGGCAATTAGCTTTATTTTTGC
GGTCACCATTGGCAGCTTTCTCAACGTGGTGATCCACCGTTTACCGGTGATGATGAAACGTGAATGGCAAAGTGAATGTA
ATTTTTACTTAAATGAATATCATCCTGATATTGTCGAGCAAATAGGTAATGAGCGCTTAAATAAACCCATTGATGCTTTT
CCTGCTAAATACAATATTGTAGTACCAGGTTCTGCCTGCCCTAAATGCAAAGCCAATATTAAACCCTGGCATAATTTACC
GATACTCGGTTGGTTGATGCTTAAAGGTAAATGTGCCGATTGCAAAACGAGTATCTCAAGCCGCTATCCCATTATTGAAC
TATTAACCGGTGTATTAATTGCTGCATTAGCCTGGCATTTTGGCCCTACATGGCAGTTTGTCTATGCCAGCATATTAACG
TTTGTACTAGTGGCGTTAACGGGTATCGATTTAGATGAAATGTTATTACCCGACCAGCTCACCTTACCTATCCTTTGGCT
AGGATTAATCATTAACCTCAAGGGCATTTATACTAGCCCAGAAGATGCCATTATCGGTGCGGCAGCAGGGTATTTAAGTC
TATGGTCAGTATTTTGGTTATTTAAATTAATCACTGGCAAGGAAGGCATGGGCTACGGTGACTTTAAACTATTAGCCGTT
TTTGGTGCCTGGTTAGGCTGGCAAATGTTACCGCTAATTATTCTATTATCTTCACTAGTAGGCGCAGTAGTGGGTATCAC
CATGATAATGGCTAAGAAGCTCAACAAAGGTAATCCAATCCCCTTTGGTCCTTATATCGCTGCAGCAGGCTGGATAGCGT
TAGTATGGGGACATGACATTCTTAATTGGTACCTTAGTACATTATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (158-266)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-147)

3D structure

• AlphaFold DB: A0A3G8LQG4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	49.837	99.675	0.497
pilD	Vibrio campbellii strain DS40M4	50.847	95.779	0.487
pilD	Acinetobacter nosocomialis M2	48.852	99.026	0.484
pilD	Vibrio cholerae strain A1552	49.492	95.779	0.474
pilD	Neisseria gonorrhoeae MS11	44.674	94.481	0.422