Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DALK_RS17030 Genome accession   NC_011768
Coordinates   4201004..4202086 (-) Length   360 a.a.
NCBI ID   WP_015948050.1    Uniprot ID   A0A1M6WNK1
Organism   Desulfatibacillum aliphaticivorans     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4196004..4207086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DALK_RS17005 (Dalk_3296) - 4196841..4197284 (-) 444 WP_015948044.1 hypothetical protein -
  DALK_RS17010 (Dalk_3297) - 4197323..4197910 (-) 588 WP_015948045.1 response regulator -
  DALK_RS17015 (Dalk_3298) - 4197968..4198888 (-) 921 WP_015948046.1 MBL fold metallo-hydrolase -
  DALK_RS29860 (Dalk_3299) - 4198913..4199065 (-) 153 WP_015948047.1 hypothetical protein -
  DALK_RS17020 (Dalk_3300) - 4199062..4199757 (-) 696 WP_015948048.1 zinc-ribbon domain-containing protein -
  DALK_RS17025 (Dalk_3301) - 4199798..4200967 (-) 1170 WP_015948049.1 type IV pilus twitching motility protein PilT -
  DALK_RS17030 (Dalk_3302) pilT 4201004..4202086 (-) 1083 WP_015948050.1 type IV pilus twitching motility protein PilT Machinery gene
  DALK_RS17035 (Dalk_3303) - 4202090..4202599 (-) 510 WP_015948051.1 DivIVA domain-containing protein -
  DALK_RS17040 (Dalk_3304) - 4202650..4202952 (-) 303 WP_015948052.1 YggT family protein -
  DALK_RS29100 (Dalk_3305) rpmF 4203459..4203635 (+) 177 WP_015948053.1 50S ribosomal protein L32 -
  DALK_RS17055 (Dalk_3307) fabG 4204198..4204944 (+) 747 WP_015948055.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  DALK_RS17060 (Dalk_3308) acpP 4205139..4205369 (+) 231 WP_015948056.1 acyl carrier protein -
  DALK_RS17065 (Dalk_3309) fabF 4205441..4206691 (+) 1251 WP_015948057.1 beta-ketoacyl-ACP synthase II -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39977.13 Da        Isoelectric Point: 7.2650

>NTDB_id=32341 DALK_RS17030 WP_015948050.1 4201004..4202086(-) (pilT) [Desulfatibacillum aliphaticivorans]
MARIDAFFKLMHEQGASDLHLVSGQQPALRIRGEMERIKFKVLDSDDLRSMLYEITPEEKVKVFEETGDLDFGYELPGVA
RYRANFFMQKFGVAAVFREIPQTILTAEQLGLPPVVPKLASLPRGLVLVTGPTGSGKSTTLASIVDVANRQRKDHIITIE
DPIEFVHESKSCIVNHREVGAHTKTFSAALRGALREDPDIILVGEMRDLETISLAIEAASTGHLVFATLHTTSAAKTVDR
IIEVFPATQQDQIRSTLADGLRAVLAQTLFKRVDKKGRCAALEILIATPAVRNLIRENKTYQIPSSIQTGKKYGMQLLDD
AIMELIHKGRIDPEEAYAKANEKARFRPLLKHPPADFTEA

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=32341 DALK_RS17030 WP_015948050.1 4201004..4202086(-) (pilT) [Desulfatibacillum aliphaticivorans]
ATGGCCAGAATTGATGCGTTTTTCAAATTAATGCATGAGCAGGGCGCTTCGGACCTTCACCTAGTATCGGGGCAGCAGCC
TGCCTTACGCATCCGGGGGGAGATGGAGAGAATCAAGTTTAAGGTCCTGGACAGCGATGACCTGCGATCCATGCTTTATG
AAATCACTCCGGAGGAAAAGGTCAAGGTGTTTGAAGAAACCGGAGACCTGGACTTTGGGTATGAACTGCCCGGAGTGGCC
CGTTACCGCGCCAACTTTTTCATGCAAAAATTCGGCGTGGCGGCGGTTTTTCGCGAAATCCCCCAGACCATTCTCACGGC
CGAACAGTTAGGCCTGCCCCCTGTCGTGCCCAAGTTGGCCAGCCTCCCCAGGGGCCTTGTGCTGGTCACCGGCCCTACAG
GATCGGGTAAGTCCACCACCCTGGCTTCCATCGTGGACGTAGCCAACCGTCAGCGCAAGGACCACATCATCACCATTGAA
GACCCCATCGAATTCGTGCACGAGTCCAAAAGCTGCATCGTGAACCACCGGGAAGTGGGCGCGCACACCAAAACTTTTTC
CGCGGCGTTGCGCGGCGCCCTGCGCGAGGACCCGGACATCATCCTGGTCGGCGAAATGCGCGACCTGGAAACCATCTCCC
TGGCCATTGAAGCCGCCTCCACAGGCCACTTGGTGTTTGCAACCCTGCATACCACCAGCGCCGCCAAAACCGTGGACCGC
ATCATAGAAGTTTTTCCGGCCACGCAGCAGGACCAGATCCGGTCCACCCTGGCGGACGGACTCAGGGCGGTTCTGGCCCA
AACCCTGTTCAAACGTGTGGATAAAAAAGGCCGCTGTGCGGCCCTGGAAATATTAATTGCTACTCCGGCGGTCAGGAACC
TGATTCGTGAAAATAAAACCTATCAGATTCCTTCCTCCATCCAGACCGGTAAGAAATACGGTATGCAATTGTTGGACGAC
GCCATTATGGAACTCATCCACAAAGGAAGAATCGATCCCGAAGAAGCCTACGCCAAGGCGAACGAGAAAGCAAGGTTCAG
ACCCTTGCTCAAACATCCGCCGGCGGACTTCACGGAGGCGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1M6WNK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

57.186

92.778

0.531
  pilT Pseudomonas aeruginosa PAK

56.707

91.111

0.517
  pilT Acinetobacter baumannii D1279779

54.192

92.778

0.503
  pilT Acinetobacter baumannii strain A118

54.192

92.778

0.503
  pilT Acinetobacter nosocomialis M2

54.192

92.778

0.503
  pilT Acinetobacter baylyi ADP1

54.683

91.944

0.503
  pilT Legionella pneumophila strain Lp02

54.683

91.944

0.503
  pilT Legionella pneumophila strain ERS1305867

54.683

91.944

0.503
  pilT Neisseria meningitidis 8013

54.154

90.278

0.489
  pilT Neisseria gonorrhoeae MS11

53.846

90.278

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.211

90.833

0.483
  pilT Vibrio cholerae strain A1552

53.211

90.833

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.271

95.278

0.469
  pilU Pseudomonas stutzeri DSM 10701

38.329

96.389

0.369
  pilU Acinetobacter baylyi ADP1

38.439

96.111

0.369

Multiple sequence alignment