Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PCC7424_RS20565 Genome accession   NC_011729
Coordinates   4633461..4634579 (+) Length   372 a.a.
NCBI ID   WP_015956138.1    Uniprot ID   B7KLI1
Organism   Gloeothece citriformis PCC 7424     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4628461..4639579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCC7424_RS20555 (PCC7424_4181) grpE 4630271..4631131 (-) 861 WP_015956136.1 nucleotide exchange factor GrpE -
  PCC7424_RS20560 (PCC7424_4182) pilB/pilB1 4631364..4633394 (+) 2031 WP_015956137.1 GspE/PulE family protein Machinery gene
  PCC7424_RS20565 (PCC7424_4183) pilT 4633461..4634579 (+) 1119 WP_015956138.1 type IV pilus twitching motility protein PilT Machinery gene
  PCC7424_RS20570 (PCC7424_4184) - 4634676..4635890 (+) 1215 WP_015956139.1 type II secretion system F family protein -
  PCC7424_RS20575 (PCC7424_4185) - 4635917..4636498 (+) 582 WP_015956140.1 hypothetical protein -
  PCC7424_RS20580 (PCC7424_4186) - 4636774..4637208 (+) 435 Protein_4166 helix-turn-helix domain-containing protein -
  PCC7424_RS20585 (PCC7424_4187) hpf 4637424..4638023 (-) 600 WP_015956142.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PCC7424_RS20590 (PCC7424_4188) lipB 4638519..4639160 (+) 642 WP_015956143.1 lipoyl(octanoyl) transferase LipB -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40918.21 Da        Isoelectric Point: 6.9581

>NTDB_id=32151 PCC7424_RS20565 WP_015956138.1 4633461..4634579(+) (pilT) [Gloeothece citriformis PCC 7424]
MAVDFLIEDLMEQLVEMGGSDMHIQAGAPIYFRISGRLNPIDDEVLSPQDSQKLIFSMLNNTQRKELEQNWELDCSYGVK
GLARFRVNVYKERGCYAACLRALSSKIPNFEQLALPNIVREMAERPRGMILVTGQTGSGKTTTLAAILDLINRTRSEHIL
TVEDPIEYVFPNVKSLFHQRQKGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVLGTLHTSSAAGT
IDRIIDVFPANQQAQIRAMLSNSLLAVFSQCLVKKKNPKPGEFGRAMAQEIMIVTPAIANLIREAKAAQIYSAIQTGMKL
GMQTMEQALANLIISGTISLEEGLSKSSKPDELQRLIAGAQFGNTNNKAKAR

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=32151 PCC7424_RS20565 WP_015956138.1 4633461..4634579(+) (pilT) [Gloeothece citriformis PCC 7424]
ATGGCAGTCGATTTTTTAATCGAAGATTTAATGGAACAACTCGTAGAAATGGGGGGTTCTGATATGCACATTCAAGCGGG
TGCGCCGATTTATTTTCGGATTAGTGGTCGCTTAAATCCGATTGATGATGAAGTTCTTTCTCCTCAAGATAGTCAGAAAT
TAATTTTTAGTATGCTCAACAATACTCAACGGAAAGAATTAGAGCAAAACTGGGAATTAGACTGTTCTTATGGGGTTAAA
GGATTAGCTCGTTTTCGGGTTAATGTGTATAAAGAGCGGGGATGTTATGCGGCTTGTTTAAGAGCCTTATCTTCTAAAAT
TCCTAATTTTGAACAGTTGGCTTTACCGAATATTGTCCGCGAAATGGCAGAAAGACCGAGAGGAATGATTTTAGTAACGG
GTCAAACTGGGTCAGGAAAAACTACCACGTTAGCGGCTATTTTAGACTTAATTAATCGGACTCGTTCAGAACATATTTTA
ACGGTAGAAGATCCCATTGAATATGTTTTTCCTAATGTTAAGAGTTTATTTCATCAACGGCAAAAGGGAGAAGATACTAA
AAGTTTTGCCAATGCTTTAAAGGCAGCCTTACGGGAAGATCCGGATATTATTCTCGTGGGGGAAATGCGGGATTTAGAAA
CAATTTCTCTAGCTATTTCTGCGGCAGAAACAGGACACCTCGTTTTAGGAACATTACACACCAGTTCGGCGGCGGGGACT
ATTGACCGGATTATTGATGTGTTTCCCGCTAACCAACAAGCTCAAATTAGAGCCATGTTATCTAACTCTTTATTAGCGGT
ATTTAGTCAATGTTTAGTGAAAAAGAAAAACCCTAAACCGGGGGAATTTGGACGAGCAATGGCACAAGAAATTATGATAG
TTACTCCCGCTATTGCTAACTTAATTAGAGAAGCTAAAGCCGCTCAAATTTATTCAGCAATTCAAACGGGTATGAAATTA
GGAATGCAAACGATGGAACAAGCTTTAGCCAATTTAATCATTTCTGGAACAATTTCTCTTGAAGAAGGGTTATCTAAAAG
TAGTAAACCTGATGAATTACAACGTCTTATTGCTGGGGCACAATTTGGAAATACTAACAATAAAGCTAAAGCTCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(54-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7KLI1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

53.333

92.742

0.495
  pilT Legionella pneumophila strain ERS1305867

53.333

92.742

0.495
  pilT Acinetobacter nosocomialis M2

51.009

93.28

0.476
  pilT Acinetobacter baumannii strain A118

51.009

93.28

0.476
  pilT Acinetobacter baumannii D1279779

51.009

93.28

0.476
  pilT Acinetobacter baylyi ADP1

50.72

93.28

0.473
  pilT Pseudomonas aeruginosa PAK

50.72

93.28

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.32

91.667

0.47
  pilT Vibrio cholerae strain A1552

51.32

91.667

0.47
  pilT Pseudomonas stutzeri DSM 10701

49.856

93.28

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.779

95.968

0.449
  pilT Neisseria gonorrhoeae MS11

49.552

90.054

0.446
  pilT Neisseria meningitidis 8013

49.552

90.054

0.446
  pilU Pseudomonas stutzeri DSM 10701

38.462

94.355

0.363

Multiple sequence alignment