Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PCC7424_RS20560 Genome accession   NC_011729
Coordinates   4631364..4633394 (+) Length   676 a.a.
NCBI ID   WP_015956137.1    Uniprot ID   B7KLI0
Organism   Gloeothece citriformis PCC 7424     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4626364..4638394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCC7424_RS31475 (PCC7424_4179) - 4627388..4627543 (+) 156 WP_015956134.1 hypothetical protein -
  PCC7424_RS20550 (PCC7424_4180) dnaK 4627615..4629786 (-) 2172 WP_015956135.1 molecular chaperone DnaK -
  PCC7424_RS20555 (PCC7424_4181) grpE 4630271..4631131 (-) 861 WP_015956136.1 nucleotide exchange factor GrpE -
  PCC7424_RS20560 (PCC7424_4182) pilB/pilB1 4631364..4633394 (+) 2031 WP_015956137.1 GspE/PulE family protein Machinery gene
  PCC7424_RS20565 (PCC7424_4183) pilT 4633461..4634579 (+) 1119 WP_015956138.1 type IV pilus twitching motility protein PilT Machinery gene
  PCC7424_RS20570 (PCC7424_4184) - 4634676..4635890 (+) 1215 WP_015956139.1 type II secretion system F family protein -
  PCC7424_RS20575 (PCC7424_4185) - 4635917..4636498 (+) 582 WP_015956140.1 hypothetical protein -
  PCC7424_RS20580 (PCC7424_4186) - 4636774..4637208 (+) 435 Protein_4166 helix-turn-helix domain-containing protein -
  PCC7424_RS20585 (PCC7424_4187) hpf 4637424..4638023 (-) 600 WP_015956142.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 676 a.a.        Molecular weight: 75422.45 Da        Isoelectric Point: 5.6325

>NTDB_id=32150 PCC7424_RS20560 WP_015956137.1 4631364..4633394(+) (pilB/pilB1) [Gloeothece citriformis PCC 7424]
MTYSTSAKKSRAIVKSGLSQLTPIGNKLVQAGYVAPEQMQQALVETRKSGRTLVDVLQNITGRQMPPDLQRQYKKHHLFE
LKVLYGVDCVDPEINQVHETDMARLVDTLIPIDICRRYKLIPLQKIETEPVGILVAMVDPDNLEAMDDINRILRPRNLAL
HRQVITLQDYEHLLEKFHDAQVIIEEEKQKIAKEKELEKLGNLTDIVDSLDLGSDRDSDEDTADDLGEDDAGKAPVINLV
NKILAKALQEGTSDIHVEPQEESLRVRFRKDGVLHQAFDPLPRQITPAVVARFKIMADLDIAERRLPQDGKIRRMYQGRK
VDFRVNTLPSRYGEKVCLRILDNSATQLGLDKLITDQKTLQIVRDLASRPFGLILVTGPTGSGKSTSLYSVLAERNHPGV
NISTAEDPIEYSLPGITQVQVIREKGMDFSSILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGA
IARLDEMGVEPFMISGALLGVLAQRLMRRVCTECRVPYNPDKAELARFGLSASNNDEVTFYKAKALTLDELAQAKQQGTV
CSKCGGKGYKGRVGVYEVMQNSERLQKLINEGATTDRIKDAAVEEGMTTLLAYSLNLVQEGYTTLEEVERVTFTDSGLEA
ELKAKRKSGLICRSCQADLVPEWLDCPYCMTPRFSE

Nucleotide


Download         Length: 2031 bp        

>NTDB_id=32150 PCC7424_RS20560 WP_015956137.1 4631364..4633394(+) (pilB/pilB1) [Gloeothece citriformis PCC 7424]
ATGACTTATTCTACATCCGCCAAGAAAAGCCGAGCTATCGTTAAAAGTGGCCTCTCCCAACTTACTCCCATTGGTAATAA
GTTGGTTCAAGCCGGTTATGTTGCCCCAGAGCAAATGCAACAAGCGTTAGTCGAAACCCGTAAATCAGGCCGCACGTTAG
TCGATGTTTTACAAAACATTACGGGGCGACAGATGCCCCCTGACTTGCAGCGACAATACAAAAAACATCATTTATTTGAA
CTCAAAGTTCTCTACGGTGTAGATTGTGTCGATCCGGAAATTAATCAAGTCCATGAAACCGACATGGCCCGATTGGTTGA
TACCCTAATTCCGATCGATATTTGTCGGCGTTATAAGCTGATTCCTCTACAAAAGATAGAGACTGAACCGGTGGGCATTT
TAGTCGCTATGGTCGATCCTGACAATTTGGAGGCTATGGATGATATTAACCGCATTTTACGCCCACGAAATCTGGCTTTA
CATCGACAGGTCATTACTCTACAAGATTATGAGCATTTACTCGAAAAATTCCATGATGCTCAGGTCATTATCGAAGAAGA
AAAGCAAAAGATAGCCAAAGAAAAAGAACTCGAAAAATTAGGGAATTTAACCGATATTGTCGATAGTCTGGATCTGGGAT
CTGATCGAGATAGTGACGAGGATACCGCAGATGATCTCGGCGAAGATGATGCCGGTAAAGCTCCGGTCATTAATCTAGTC
AATAAAATTTTGGCTAAAGCACTCCAAGAAGGAACTTCTGATATTCACGTTGAACCGCAAGAAGAGTCTTTACGGGTGCG
TTTTCGTAAGGATGGGGTACTCCATCAAGCGTTTGATCCCCTCCCTCGACAAATTACTCCGGCAGTAGTCGCCCGTTTCA
AGATTATGGCAGATTTGGACATTGCGGAAAGACGACTGCCTCAAGATGGTAAAATCCGCCGAATGTACCAAGGCCGAAAA
GTCGATTTTCGGGTCAATACGTTACCGAGTCGCTATGGGGAAAAAGTCTGTTTACGGATCTTGGATAACTCAGCGACTCA
ATTAGGATTAGATAAGCTCATTACTGACCAAAAAACCTTACAAATTGTTCGGGATCTTGCTAGTCGTCCTTTTGGGTTGA
TTTTAGTCACTGGGCCGACGGGGTCAGGAAAATCCACCAGTTTATACTCGGTTTTGGCGGAACGGAATCACCCAGGAGTC
AATATTAGTACGGCAGAAGACCCGATCGAATATTCTTTACCCGGAATTACTCAAGTTCAAGTCATTCGGGAAAAAGGAAT
GGATTTTTCGTCTATTCTGCGGGCGTTTATGCGTCAAGACCCTGATGTCATCCTCGTGGGTGAAACCCGAGATAAGGAAA
CCGCTAAAACCGCGATTGAAGCCGCCTTAACCGGTCACTTGGTTTTAACCACCCTCCATACTAATGATGCCGCCGGCGCG
ATCGCTCGTTTGGATGAAATGGGGGTTGAACCGTTTATGATTTCGGGTGCATTATTGGGAGTTTTGGCACAACGGTTAAT
GCGGCGAGTCTGTACGGAATGTCGAGTTCCTTATAATCCTGACAAAGCGGAATTAGCTCGGTTTGGCTTATCGGCTTCTA
ATAATGATGAAGTGACTTTTTACAAAGCAAAGGCTTTAACCTTGGATGAACTTGCTCAAGCTAAACAACAAGGTACAGTC
TGTTCTAAATGTGGCGGAAAAGGCTATAAAGGTCGGGTTGGGGTTTATGAAGTGATGCAAAATAGCGAACGCCTGCAAAA
ATTAATCAATGAAGGAGCAACAACCGATCGCATTAAGGATGCTGCTGTAGAAGAAGGGATGACGACCTTATTAGCTTACA
GTTTAAATCTAGTGCAAGAAGGTTACACCACCTTAGAAGAAGTGGAACGGGTGACATTTACAGATTCAGGTTTAGAGGCT
GAACTAAAAGCTAAACGGAAGAGTGGGTTAATTTGTCGCTCTTGTCAAGCGGATTTAGTTCCAGAATGGCTTGATTGTCC
TTATTGTATGACTCCTCGTTTTAGTGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(242-509)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7KLI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

71.704

99.852

0.716

Multiple sequence alignment