Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D8M16_RS03675 Genome accession   NZ_CP032841
Coordinates   754660..755640 (+) Length   326 a.a.
NCBI ID   WP_121478589.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain C15117     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 749660..760640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8M16_RS03635 - 749688..750014 (+) 327 WP_003862421.1 YggL family protein -
  D8M16_RS03640 - 750072..750788 (+) 717 WP_121478587.1 DUF2884 domain-containing protein -
  D8M16_RS03645 - 750903..751265 (-) 363 WP_121478588.1 endonuclease domain-containing protein -
  D8M16_RS03650 hemW 751322..752470 (-) 1149 WP_063133030.1 radical SAM family heme chaperone HemW -
  D8M16_RS03655 - 752463..753056 (-) 594 WP_015571839.1 XTP/dITP diphosphatase -
  D8M16_RS03660 yggU 753060..753356 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  D8M16_RS03665 - 753353..753919 (-) 567 WP_015571838.1 YggT family protein -
  D8M16_RS03670 - 753941..754642 (-) 702 WP_015571837.1 YggS family pyridoxal phosphate-dependent enzyme -
  D8M16_RS03675 pilT 754660..755640 (+) 981 WP_121478589.1 type IV pilus twitching motility protein PilT Machinery gene
  D8M16_RS03680 ruvX 755651..756067 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  D8M16_RS03685 - 756067..756627 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  D8M16_RS03690 gshB 756702..757649 (-) 948 WP_121478590.1 glutathione synthase -
  D8M16_RS03695 rsmE 757681..758412 (-) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  D8M16_RS03700 endA 758464..759171 (-) 708 WP_003860034.1 deoxyribonuclease I -
  D8M16_RS03705 - 759266..759763 (-) 498 WP_121478591.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35674.60 Da        Isoelectric Point: 6.1742

>NTDB_id=319083 D8M16_RS03675 WP_121478589.1 754660..755640(+) (pilT) [Enterobacter hormaechei strain C15117]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAHAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=319083 D8M16_RS03675 WP_121478589.1 754660..755640(+) (pilT) [Enterobacter hormaechei strain C15117]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCACCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGACGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCTAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCATCAGACAGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTACG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCATACGCGCGGCGCGGCGCATGCGATTGAGCGCCTGGTCGATACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACGTCGGCGGCAGCAAATCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374

Multiple sequence alignment