Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SSPA_RS14995 Genome accession   NC_011147
Coordinates   3064979..3065959 (-) Length   326 a.a.
NCBI ID   WP_001055655.1    Uniprot ID   A0A5Z0RZB6
Organism   Salmonella enterica subsp. enterica serovar Paratyphi A str. AKU_12601     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3059979..3070959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SSPA_RS14965 (SSPA2755) endA 3060437..3061144 (+) 708 WP_000286124.1 deoxyribonuclease I -
  SSPA_RS14970 (SSPA2756) rsmE 3061221..3061952 (+) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SSPA_RS14975 (SSPA2757) gshB 3061972..3062919 (+) 948 WP_000593248.1 glutathione synthase -
  SSPA_RS14980 (SSPA2758) - 3063135..3063698 (+) 564 WP_001053176.1 YqgE/AlgH family protein -
  SSPA_RS14985 (SSPA2759) ruvX 3063698..3064114 (+) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  SSPA_RS14990 (SSPA2760) - 3064161..3064847 (-) 687 WP_000098319.1 IclR family transcriptional regulator domain-containing protein -
  SSPA_RS14995 (SSPA2761) pilT 3064979..3065959 (-) 981 WP_001055655.1 type IV pilus twitching motility protein PilT Machinery gene
  SSPA_RS15000 (SSPA2762) - 3065977..3066681 (+) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  SSPA_RS15005 (SSPA2763) - 3066700..3067266 (+) 567 WP_001094848.1 YggT family protein -
  SSPA_RS15010 (SSPA2764) yggU 3067263..3067553 (+) 291 WP_001277203.1 DUF167 family protein YggU -
  SSPA_RS15015 (SSPA2765) - 3067561..3068154 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  SSPA_RS15020 (SSPA2766) hemW 3068147..3069283 (+) 1137 WP_001096530.1 radical SAM family heme chaperone HemW -
  SSPA_RS15025 (SSPA2767) - 3069374..3070381 (-) 1008 WP_000252189.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36139.35 Da        Isoelectric Point: 7.8164

>NTDB_id=31469 SSPA_RS14995 WP_001055655.1 3064979..3065959(-) (pilT) [Salmonella enterica subsp. enterica serovar Paratyphi A str. AKU_12601]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFKHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREVGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQEGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=31469 SSPA_RS14995 WP_001055655.1 3064979..3065959(-) (pilT) [Salmonella enterica subsp. enterica serovar Paratyphi A str. AKU_12601]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCAAACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAGTCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGAGGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Z0RZB6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365

Multiple sequence alignment