Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   D1345_RS01830 Genome accession   NZ_CP031968
Coordinates   406569..407006 (+) Length   145 a.a.
NCBI ID   WP_118266438.1    Uniprot ID   -
Organism   Chromobacterium rhizoryzae strain JP2-74     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401569..412006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1345_RS01810 (D1345_01810) proB 401669..402787 (-) 1119 WP_043592290.1 glutamate 5-kinase -
  D1345_RS01815 (D1345_01815) - 402987..404489 (-) 1503 WP_157221998.1 hypothetical protein -
  D1345_RS01820 (D1345_01820) - 404591..405943 (-) 1353 WP_118266433.1 adenosylmethionine--8-amino-7-oxononanoate transaminase -
  D1345_RS24595 (D1345_01825) pilA2 406137..406556 (+) 420 WP_118266435.1 pilin Machinery gene
  D1345_RS01830 (D1345_01830) pilA 406569..407006 (+) 438 WP_118266438.1 pilin Machinery gene
  D1345_RS01835 (D1345_01835) - 406957..408879 (+) 1923 WP_162896944.1 Wzy polymerase domain-containing protein -
  D1345_RS01840 (D1345_01840) - 408955..410739 (+) 1785 WP_118266443.1 O-antigen ligase family protein -
  D1345_RS01845 (D1345_01845) rpoH 410812..411675 (-) 864 WP_019102027.1 RNA polymerase sigma factor RpoH -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14896.85 Da        Isoelectric Point: 8.4688

>NTDB_id=312951 D1345_RS01830 WP_118266438.1 406569..407006(+) (pilA) [Chromobacterium rhizoryzae strain JP2-74]
MQKSQQGFTLIELMIVVAIIGILAAIAIPAYQDYTKRARVSEGLSLASAAKTAVAEYYASNGSFPSTNTSAGLQTSISGN
SVTSVLVGTSGVITVSYSTTLIDSTKNQLTLTPVASNGSIVWTCGASGTNALNSQWLPANCRAGN

Nucleotide


Download         Length: 438 bp        

>NTDB_id=312951 D1345_RS01830 WP_118266438.1 406569..407006(+) (pilA) [Chromobacterium rhizoryzae strain JP2-74]
ATGCAAAAATCCCAACAAGGTTTTACTCTTATCGAACTGATGATAGTGGTGGCTATTATTGGTATTCTCGCCGCGATTGC
TATTCCCGCTTATCAGGACTATACCAAACGGGCTAGGGTGTCAGAGGGGCTAAGCTTGGCTTCTGCAGCCAAAACTGCAG
TGGCAGAGTACTATGCTTCAAATGGTTCTTTCCCCTCTACAAATACTTCAGCTGGCTTGCAAACATCAATTAGTGGTAAC
TCAGTGACTAGTGTGCTTGTTGGTACAAGCGGTGTCATTACGGTTTCATATAGCACTACTTTGATTGACTCCACCAAAAA
TCAATTGACGCTGACACCTGTTGCATCGAATGGGTCGATTGTTTGGACATGTGGAGCGTCTGGGACTAATGCCCTTAACT
CTCAGTGGTTGCCAGCTAACTGCCGTGCCGGCAACTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

45.399

100

0.51
  pilA2 Legionella pneumophila str. Paris

51.079

95.862

0.49
  pilA2 Legionella pneumophila strain ERS1305867

51.079

95.862

0.49
  pilE Neisseria gonorrhoeae MS11

43.75

100

0.483
  pilE Neisseria gonorrhoeae strain FA1090

44.025

100

0.483
  comP Acinetobacter baylyi ADP1

45.946

100

0.469
  pilA/pilA1 Eikenella corrodens VA1

40.506

100

0.441
  pilA Haemophilus influenzae 86-028NP

41.549

97.931

0.407
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.278

99.31

0.4
  pilA Haemophilus influenzae Rd KW20

40

100

0.4
  pilA Vibrio cholerae C6706

37.5

100

0.393
  pilA Vibrio cholerae strain A1552

37.5

100

0.393
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.5

100

0.393
  pilA Vibrio parahaemolyticus RIMD 2210633

43.75

88.276

0.386
  pilA Pseudomonas aeruginosa PAK

34.641

100

0.366

Multiple sequence alignment