Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AXA52_RS01235 Genome accession   NZ_CP031574
Coordinates   248544..249524 (-) Length   326 a.a.
NCBI ID   WP_017382924.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain A1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 243544..254524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXA52_RS01205 (AXA52_01205) - 244421..244918 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  AXA52_RS01210 (AXA52_01210) endA 245013..245720 (+) 708 WP_003860034.1 deoxyribonuclease I -
  AXA52_RS01215 (AXA52_01215) rsmE 245772..246503 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AXA52_RS01220 (AXA52_01220) gshB 246535..247482 (+) 948 WP_015571835.1 glutathione synthase -
  AXA52_RS01225 (AXA52_01225) - 247557..248117 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  AXA52_RS01230 (AXA52_01230) ruvX 248117..248533 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  AXA52_RS01235 (AXA52_01235) pilT 248544..249524 (-) 981 WP_017382924.1 type IV pilus twitching motility protein PilT Machinery gene
  AXA52_RS01240 (AXA52_01240) - 249542..250243 (+) 702 WP_017382925.1 YggS family pyridoxal phosphate-dependent enzyme -
  AXA52_RS01245 (AXA52_01245) - 250265..250831 (+) 567 WP_015571838.1 YggT family protein -
  AXA52_RS01250 (AXA52_01250) yggU 250828..251124 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  AXA52_RS01255 (AXA52_01255) - 251128..251721 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  AXA52_RS01260 (AXA52_01260) hemW 251714..252862 (+) 1149 WP_116480936.1 radical SAM family heme chaperone HemW -
  AXA52_RS01265 (AXA52_01265) - 252919..253257 (+) 339 WP_017382927.1 endonuclease domain-containing protein -
  AXA52_RS01270 (AXA52_01270) - 253341..254057 (-) 717 WP_017382928.1 DUF2884 domain-containing protein -
  AXA52_RS01275 (AXA52_01275) - 254114..254440 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35665.59 Da        Isoelectric Point: 6.0405

>NTDB_id=307724 AXA52_RS01235 WP_017382924.1 248544..249524(-) (pilT) [Enterobacter hormaechei strain A1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=307724 AXA52_RS01235 WP_017382924.1 248544..249524(-) (pilT) [Enterobacter hormaechei strain A1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGACGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCTAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCATCAGACAGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTACG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATTGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACGTCGGCGGCGGCCAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(72-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377

Multiple sequence alignment