Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EXIG_RS10840 Genome accession   NC_010556
Coordinates   2075441..2076484 (-) Length   347 a.a.
NCBI ID   WP_012370992.1    Uniprot ID   -
Organism   Exiguobacterium sibiricum 255-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2070441..2081484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXIG_RS10820 (Exig_2119) - 2070979..2072730 (-) 1752 WP_012370988.1 fimbrial assembly protein -
  EXIG_RS10825 (Exig_2120) - 2072749..2073507 (-) 759 WP_012370989.1 A24 family peptidase -
  EXIG_RS10830 (Exig_2121) - 2073562..2074080 (-) 519 WP_012370990.1 type II secretion system protein -
  EXIG_RS10835 (Exig_2122) - 2074219..2075436 (-) 1218 WP_026827808.1 type II secretion system F family protein -
  EXIG_RS10840 (Exig_2123) pilT 2075441..2076484 (-) 1044 WP_012370992.1 type IV pilus twitching motility protein PilT Machinery gene
  EXIG_RS10845 (Exig_2124) - 2076481..2078145 (-) 1665 WP_012370993.1 GspE/PulE family protein -
  EXIG_RS10850 (Exig_2125) - 2078154..2078468 (-) 315 WP_012370994.1 hypothetical protein -
  EXIG_RS10855 (Exig_2126) - 2078495..2079856 (-) 1362 WP_148206630.1 G5 domain-containing protein -
  EXIG_RS10860 (Exig_2127) - 2080032..2080556 (+) 525 WP_012370996.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EXIG_RS10865 (Exig_2128) - 2080519..2081133 (+) 615 WP_012370997.1 type II secretion system protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38146.97 Da        Isoelectric Point: 7.0664

>NTDB_id=30530 EXIG_RS10840 WP_012370992.1 2075441..2076484(-) (pilT) [Exiguobacterium sibiricum 255-15]
MSTMERAQIEDILRSSKEKEASDVHLTAGSPPVFRINGVLTPFGSEKMKPIIIEGFVRSLITEEMFLQLKQDRDIDFSFG
VTGVARYRVNCFYQRGALSMAFRTIPTEVPTLDQLSLPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYKR
IITLEDPIEYLHSHQKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETISTAVTAAETGHLVFATVHTSTAA
STVSRIIDVFPSEQQDQIRAQLANVLAGVVSQRLMPRTDGTGRVAALEIMVETPAVSNLIRTGKEYQIQSVLQTGKQYGM
QTMQMALQDLVSRGLIPASAATPYISG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=30530 EXIG_RS10840 WP_012370992.1 2075441..2076484(-) (pilT) [Exiguobacterium sibiricum 255-15]
ATGAGTACGATGGAACGCGCCCAAATTGAAGACATTTTACGATCGTCAAAAGAAAAAGAAGCATCTGATGTCCATCTGAC
GGCCGGCTCACCACCGGTCTTTCGCATCAACGGTGTCCTGACACCGTTTGGCTCGGAAAAGATGAAGCCGATCATCATTG
AAGGGTTCGTCCGTTCCCTTATTACCGAGGAAATGTTTTTACAGTTAAAACAGGATCGCGATATCGACTTTTCATTTGGT
GTGACAGGAGTGGCGCGCTACCGCGTCAATTGTTTTTATCAACGCGGTGCGTTGTCGATGGCTTTCCGGACAATTCCGAC
CGAAGTCCCGACACTCGATCAATTGTCATTACCGCCGGTCTTAAAACGTTTTTTGACGAAGCCGCACGGTTTGATTCTCG
TGACCGGTCCGACCGGTTCCGGGAAATCGACGACACTGGCAGCGATGATCAATGAAATCAACCAGACGATGTATAAACGG
ATCATCACGCTCGAAGATCCGATTGAATATTTGCATAGTCATCAGAAAAGTCTGATTGACCAGCGGGAAATCGGAATCGA
TGCGCCGCGGTTTGCGACCGGCTTACGGTCTGCCCTCCGGCAGGATCCGGATGTCATCTTACTCGGTGAGATGCGCGATC
TGGAAACGATTTCAACCGCCGTGACGGCAGCAGAGACCGGTCATCTAGTTTTCGCGACCGTCCATACGTCGACGGCGGCA
TCGACGGTCAGCCGGATCATCGATGTCTTTCCGTCCGAGCAACAAGACCAGATTCGGGCTCAACTCGCGAACGTTCTAGC
CGGTGTCGTCTCACAACGGCTAATGCCGCGAACAGACGGAACGGGTCGTGTCGCAGCACTTGAAATCATGGTCGAGACAC
CGGCTGTGTCAAACTTGATCCGGACCGGGAAAGAATATCAGATTCAATCTGTTCTGCAGACAGGAAAACAGTACGGGATG
CAGACGATGCAGATGGCATTACAAGACTTAGTGAGTCGTGGATTGATTCCAGCATCTGCCGCGACACCTTACATATCGGG
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.524

94.524

0.487
  pilT Neisseria meningitidis 8013

49.701

96.254

0.478
  pilT Neisseria gonorrhoeae MS11

49.701

96.254

0.478
  pilT Acinetobacter nosocomialis M2

48.657

96.542

0.47
  pilT Vibrio cholerae strain A1552

49.091

95.101

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

95.101

0.467
  pilT Acinetobacter baumannii D1279779

48.358

96.542

0.467
  pilT Acinetobacter baumannii strain A118

48.358

96.542

0.467
  pilT Pseudomonas aeruginosa PAK

48.348

95.965

0.464
  pilT Acinetobacter baylyi ADP1

48.171

94.524

0.455
  pilT Pseudomonas stutzeri DSM 10701

47.147

95.965

0.452
  pilT Legionella pneumophila strain Lp02

45.946

95.965

0.441
  pilT Legionella pneumophila strain ERS1305867

45.946

95.965

0.441
  pilU Acinetobacter baylyi ADP1

40

96.542

0.386
  pilU Pseudomonas stutzeri DSM 10701

40.426

94.813

0.383

Multiple sequence alignment