Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DV427_RS05375 Genome accession   NZ_CP031240
Coordinates   1066062..1066502 (-) Length   146 a.a.
NCBI ID   WP_114891563.1    Uniprot ID   -
Organism   Haemophilus haemolyticus strain M19345     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1061062..1071502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV427_RS05355 rho 1061449..1062711 (+) 1263 WP_005629550.1 transcription termination factor Rho -
  DV427_RS05360 pilD 1062765..1063457 (-) 693 WP_114891560.1 prepilin peptidase Machinery gene
  DV427_RS05365 pilC 1063454..1064674 (-) 1221 WP_114891561.1 type II secretion system F family protein Machinery gene
  DV427_RS05370 pilB 1064671..1066065 (-) 1395 WP_114891562.1 GspE/PulE family protein Machinery gene
  DV427_RS05375 pilA 1066062..1066502 (-) 441 WP_114891563.1 prepilin peptidase-dependent pilin Machinery gene
  DV427_RS05380 ampD 1066617..1067171 (+) 555 WP_114891564.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV427_RS05385 rppH 1067697..1068287 (+) 591 WP_005631202.1 RNA pyrophosphohydrolase -
  DV427_RS05390 - 1068287..1069084 (+) 798 WP_114891565.1 sulfite exporter TauE/SafE family protein -
  DV427_RS05395 lgt 1069093..1069899 (+) 807 WP_114891566.1 prolipoprotein diacylglyceryl transferase -
  DV427_RS05400 - 1069909..1070760 (+) 852 WP_114891567.1 thymidylate synthase -
  DV427_RS05405 tadA 1070760..1071275 (+) 516 WP_114891568.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15007.13 Da        Isoelectric Point: 8.0930

>NTDB_id=304478 DV427_RS05375 WP_114891563.1 1066062..1066502(-) (pilA) [Haemophilus haemolyticus strain M19345]
MKLTTQTTLNKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKSDVELCVYSTGKPSSCSGGSNGIA
ADITTAKGYVKSVTTSNGAITVAGNGTLDGMSYTLTAEGNSANGVTWKTTCGTTNADIFPAGFCSK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=304478 DV427_RS05375 WP_114891563.1 1066062..1066502(-) (pilA) [Haemophilus haemolyticus strain M19345]
ATGAAACTAACTACACAAACTACCTTGAATAAAGGATTTACCTTAATTGAATTAATGATAGTCATCGCCATCATCGCGAT
TTTAGCCACTATCGCTATTCCCTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCTTCCGCAC
CTTATAAGTCAGATGTGGAATTATGCGTTTATAGCACAGGTAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGAATTGCG
GCTGATATTACAACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAGCGGGTAATGGTAC
ATTAGACGGAATGAGTTACACACTTACTGCTGAAGGCAATAGTGCAAACGGCGTTACATGGAAAACTACCTGTGGCACAA
CGAATGCCGATATTTTCCCTGCTGGATTCTGTTCAAAATGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

81.25

98.63

0.801
  pilA Haemophilus influenzae Rd KW20

77.778

98.63

0.767
  pilA Glaesserella parasuis strain SC1401

56

100

0.575
  pilA2 Legionella pneumophila strain ERS1305867

40.816

100

0.411
  pilE Neisseria gonorrhoeae MS11

42.754

94.521

0.404
  comP Acinetobacter baylyi ADP1

40.69

99.315

0.404
  pilA2 Legionella pneumophila str. Paris

41.259

97.945

0.404
  pilA Vibrio campbellii strain DS40M4

37.671

100

0.377
  pilA Vibrio cholerae strain A1552

37.241

99.315

0.37
  pilA Vibrio cholerae C6706

37.241

99.315

0.37
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.241

99.315

0.37
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.301

100

0.363

Multiple sequence alignment