Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DV427_RS05370 Genome accession   NZ_CP031240
Coordinates   1064671..1066065 (-) Length   464 a.a.
NCBI ID   WP_114891562.1    Uniprot ID   -
Organism   Haemophilus haemolyticus strain M19345     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1059671..1071065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV427_RS05335 - 1060033..1060226 (-) 194 Protein_1003 cation transporter -
  DV427_RS05340 - 1060201..1060407 (-) 207 WP_114891558.1 heavy-metal-associated domain-containing protein -
  DV427_RS05345 - 1060484..1060870 (+) 387 WP_114891559.1 Cu(I)-responsive transcriptional regulator -
  DV427_RS05350 metJ 1060884..1061201 (-) 318 WP_005634287.1 met regulon transcriptional regulator MetJ -
  DV427_RS05355 rho 1061449..1062711 (+) 1263 WP_005629550.1 transcription termination factor Rho -
  DV427_RS05360 pilD 1062765..1063457 (-) 693 WP_114891560.1 prepilin peptidase Machinery gene
  DV427_RS05365 pilC 1063454..1064674 (-) 1221 WP_114891561.1 type II secretion system F family protein Machinery gene
  DV427_RS05370 pilB 1064671..1066065 (-) 1395 WP_114891562.1 GspE/PulE family protein Machinery gene
  DV427_RS05375 pilA 1066062..1066502 (-) 441 WP_114891563.1 prepilin peptidase-dependent pilin Machinery gene
  DV427_RS05380 ampD 1066617..1067171 (+) 555 WP_114891564.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV427_RS05385 rppH 1067697..1068287 (+) 591 WP_005631202.1 RNA pyrophosphohydrolase -
  DV427_RS05390 - 1068287..1069084 (+) 798 WP_114891565.1 sulfite exporter TauE/SafE family protein -
  DV427_RS05395 lgt 1069093..1069899 (+) 807 WP_114891566.1 prolipoprotein diacylglyceryl transferase -
  DV427_RS05400 - 1069909..1070760 (+) 852 WP_114891567.1 thymidylate synthase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52589.04 Da        Isoelectric Point: 6.2394

>NTDB_id=304477 DV427_RS05370 WP_114891562.1 1064671..1066065(-) (pilB) [Haemophilus haemolyticus strain M19345]
MIDLTSAKPRVKAQNGEIFTISPDLWGRNQQQQSMLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKLVEPVLL
ESTQLKELLQQLAPNQLQVEEQVKYYQHQEQPHLEQQDDEPVIRLLNQIFESALQNNASDIHLETLSAHFQVRFRIDGVL
QAQPPLSKNLANRIISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKVVLRAQQNKPVELSFSELG
MTESQQKEFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=304477 DV427_RS05370 WP_114891562.1 1064671..1066065(-) (pilB) [Haemophilus haemolyticus strain M19345]
ATGATCGACTTAACTAGTGCAAAACCTCGTGTAAAAGCACAAAATGGCGAGATATTTACGATCTCGCCAGATTTATGGGG
ACGTAATCAGCAACAACAGTCTATGCTCTTACGTTATTTTGCGTTACCACTCAAAGAAGAAAATAATCGTCTTTGGTTAG
GTGTCGATTCCCTTTCTAATCTTTCCGCCTGTGAAACTATTGCGTTTATAACCGGTAAACTTGTTGAGCCAGTTTTATTA
GAAAGCACTCAACTTAAAGAACTATTACAGCAACTTGCCCCTAATCAACTTCAAGTGGAAGAGCAAGTTAAATATTACCA
ACATCAAGAACAGCCTCATCTAGAACAACAAGATGACGAGCCTGTCATTCGCTTGCTTAATCAAATTTTTGAATCTGCTT
TACAAAACAATGCCTCAGATATTCATTTAGAGACCTTATCGGCTCATTTCCAAGTGCGGTTTAGAATTGACGGTGTTTTA
CAAGCTCAGCCTCCACTTAGCAAAAATTTGGCTAATCGCATTATTTCTCGCCTAAAACTGCTCGCTAAATTAGATATCAG
TGAAACCCGCCTTCCACAAGATGGACGATTTCAATTTAAAACTACGTTTTCCGATATTCTTGATTTCCGTCTTTCAACGT
TACCAACTCATTGGGGAGAGAAAGTTGTCCTGAGAGCACAGCAAAATAAACCAGTAGAACTCAGCTTTTCTGAACTAGGC
ATGACTGAAAGTCAACAGAAAGAATTTCAACGCGCGCTTAGCCAACCACAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAACACGCCTGATAAACATATTATGACCGCAGAGGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAAATAGGACTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGCCAAGATCCAGACATTATTATGTTAGGTGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACTAATGATGCAATATCCGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAACATGAAATTGAAAACAGTTTACTGCTCGTTATTGCTCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGCCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACGGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-96)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

92.527

98.06

0.907
  pilB Haemophilus influenzae Rd KW20

91.41

97.845

0.894
  pilB Glaesserella parasuis strain SC1401

58.333

98.276

0.573
  pilB Vibrio campbellii strain DS40M4

40.862

100

0.429
  pilB Vibrio cholerae strain A1552

40.619

100

0.425
  pilB Legionella pneumophila strain ERS1305867

39.798

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.541

100

0.42
  pilB Acinetobacter baylyi ADP1

37.876

100

0.407
  pilB Acinetobacter baumannii D1279779

39.13

100

0.407
  pilF Neisseria gonorrhoeae MS11

37.395

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.1

100

0.375
  pilF Thermus thermophilus HB27

35.924

100

0.369

Multiple sequence alignment