Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DVB73_RS15195 Genome accession   NZ_CP031146
Coordinates   3318695..3319693 (-) Length   332 a.a.
NCBI ID   WP_016393368.1    Uniprot ID   A0AAD0VU34
Organism   Pseudomonas plecoglossicida strain XSDHY-P     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3313695..3324693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DVB73_RS15175 (DVB73_15170) argS 3314913..3316649 (+) 1737 WP_016393372.1 arginine--tRNA ligase -
  DVB73_RS15180 (DVB73_15175) - 3316651..3317349 (+) 699 WP_016393371.1 SPOR domain-containing protein -
  DVB73_RS15185 (DVB73_15180) - 3317395..3317814 (-) 420 WP_016393370.1 TM2 domain-containing protein -
  DVB73_RS15190 (DVB73_15185) - 3317984..3318613 (+) 630 WP_016393369.1 C40 family peptidase -
  DVB73_RS15195 (DVB73_15190) pilT 3318695..3319693 (-) 999 WP_016393368.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DVB73_RS15200 (DVB73_15195) - 3319759..3320445 (+) 687 WP_016393367.1 YggS family pyridoxal phosphate-dependent enzyme -
  DVB73_RS15205 (DVB73_15200) proC 3320484..3321302 (+) 819 WP_016393366.1 pyrroline-5-carboxylate reductase -
  DVB73_RS15210 (DVB73_15205) - 3321313..3321903 (+) 591 WP_016393365.1 YggT family protein -
  DVB73_RS15215 (DVB73_15210) - 3321992..3323131 (+) 1140 WP_016393364.1 homoserine O-acetyltransferase -
  DVB73_RS15220 (DVB73_15215) metW 3323139..3323759 (+) 621 WP_015272149.1 methionine biosynthesis protein MetW -
  DVB73_RS15225 (DVB73_15220) - 3323782..3324216 (+) 435 WP_016393363.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36160.96 Da        Isoelectric Point: 6.2560

>NTDB_id=303766 DVB73_RS15195 WP_016393368.1 3318695..3319693(-) (pilT) [Pseudomonas plecoglossicida strain XSDHY-P]
MDVTDLLARAVEAGASDLHLAAGEIPMLRLDGELQRLDLPCLQPAALNDGMAPLLDEGRRQCWEKGDEQDLALELAPLGR
FRLNLFRQLNGPAATFRLIPRRIPTLGELALEEVYRAIARYTDGLVLIGGPTGSGKSSTLAALIDQLNRERCLHIITLED
PVEVIHSGQRSLVNQREIGRHCRDFSQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSIDRL
VDVFAAEEKPLVRAMLAESLRMVVAQVLVKRGGGGRVAAREVLVATPAVRNLIREGRMAQLCSVMQAGAAEGMLTMEGAM
KQLKSEGLIELG

Nucleotide


Download         Length: 999 bp        

>NTDB_id=303766 DVB73_RS15195 WP_016393368.1 3318695..3319693(-) (pilT) [Pseudomonas plecoglossicida strain XSDHY-P]
ATGGACGTGACCGACCTGCTGGCCCGGGCTGTGGAGGCAGGGGCGAGCGACCTGCACCTGGCTGCGGGCGAAATACCGAT
GCTGCGCCTGGATGGCGAGTTGCAGCGGCTGGACCTGCCGTGCCTGCAGCCTGCTGCGTTGAACGATGGCATGGCGCCTC
TGCTCGACGAGGGGCGGCGCCAGTGCTGGGAAAAGGGTGATGAGCAGGATCTGGCCCTGGAGCTGGCGCCATTGGGACGC
TTTCGTCTCAATCTGTTTCGCCAGTTGAATGGCCCTGCGGCGACCTTTCGCCTCATTCCCAGGCGCATCCCTACACTGGG
TGAGCTGGCACTGGAAGAAGTGTATCGAGCTATTGCGCGGTATACCGATGGGCTGGTCCTGATCGGGGGGCCAACCGGCA
GCGGCAAGTCCAGCACCTTGGCGGCCCTGATCGATCAGCTCAATCGTGAGCGCTGTCTGCACATCATCACCCTCGAAGAC
CCTGTGGAAGTTATCCACAGCGGTCAGCGCAGCCTGGTCAACCAACGCGAGATCGGCCGACACTGCCGGGACTTTTCCCA
GGGCTTGCGCAGTGCGCTGCGTCAGGACCCGGATGTGATCATGATCGGCGAGTTGCGAGATCTCGAGACGATACGCCTGG
CGCTGCGGGCGGCGGAAACGGGGCACCTGGTGCTGGCAACGGTGCATACGCGCTCGGCGGTGAGCAGCATCGACCGTCTG
GTGGATGTGTTTGCGGCAGAGGAAAAGCCATTGGTACGGGCCATGTTGGCGGAGTCGCTGCGCATGGTGGTGGCGCAGGT
GCTGGTCAAGCGTGGGGGTGGTGGGCGGGTGGCGGCGAGGGAAGTGCTGGTGGCGACGCCGGCTGTGCGCAACCTGATTC
GCGAGGGGCGTATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGGGCGGCGGAGGGGATGCTGACGATGGAAGGGGCGATG
AAACAGCTGAAGAGCGAAGGGCTGATCGAGCTTGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.964

99.699

0.518
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

99.398

0.515
  pilT Vibrio cholerae strain A1552

51.818

99.398

0.515
  pilT Pseudomonas aeruginosa PAK

51.368

99.096

0.509
  pilT Pseudomonas stutzeri DSM 10701

51.064

99.096

0.506
  pilT Acinetobacter nosocomialis M2

51.064

99.096

0.506
  pilT Acinetobacter baumannii strain A118

50.76

99.096

0.503
  pilT Acinetobacter baumannii D1279779

50.76

99.096

0.503
  pilT Legionella pneumophila strain Lp02

49.544

99.096

0.491
  pilT Legionella pneumophila strain ERS1305867

49.544

99.096

0.491
  pilT Neisseria meningitidis 8013

48.485

99.398

0.482
  pilT Neisseria gonorrhoeae MS11

48.182

99.398

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.246

100

0.464
  pilU Pseudomonas stutzeri DSM 10701

37.879

99.398

0.377
  pilU Vibrio cholerae strain A1552

37.037

97.59

0.361

Multiple sequence alignment